Running a Basic 2 color Flow Cytometry Experiment in BD FACS Diva

[@laxmanegalitarian8581]

Man, you deserve to earn $150 for every full video watched. I literally took a 2 hours training on Flow Cytometry yesterday ($75 an hour, paid by the lab though) from some super speed instructor who would not wait for me to take notes even and learned literally nothing. As soon as you started explaining what all those windows were, I knew your video is going to be great. This must be the most beautifully explained video for flow cytometry on the internet. Thank you man, I wasted my lab's money yesterday and I am learning from you today. I will watch your next video on compensation too.

[@a.a.2277]

This was the clearest explanation I ever get about FACS and flow. It helped me a lot to understand. Thank you very much!

[@Biomeducated]

Most interestingly it's a good showcase on how to use FACS Diva software! Sooooo helpful!

[@DrShafi-xn6pk]

This is so far the best video I have seen on FACS DIVA to set an experiment, to set controls, and to resolve different populations

[@juanr859]

Excellent presentation. It is clear, concise and engaging. I truly appreciate your willingness to share your expertise.

[@dotheneedful55]

Thank you soo very much! I have been searching forba clear cut explanation that isnt just someone explaining the theory.

[@yuantian5320]

this tutorial is much better than the previous training I received, even that trainer speaks my native language and you explain all in English.

[@jazmincastro9248]

I can't say enough, THANK YOU SO VERY MUCH for this!!!!! FANTASTIC video and explanation

[@prakritikarki3740]

The best video for flow cytometry experiment found ever

[@sanmaybanerjee]

This is a mind-blowingly good demonstration. Seriously, mate. Thanks a lot.

[@lillianliu2084]

Very nice video and clearly explained the basic operation of FACSDiva. Though I got trained by our core facility, your video made me less frustrated for learning to use this complex machine and software.

[@ChakravarthyGarlapati]

Dude I thought of making one of these videos as so many of my friends are asking. You explained it nicely. I can simply share your video if someone asks. By the way you have nicely done it covering each and every point needed for the beginners.

[@tpankajavalli]

Excellent demo! Extremely helpful, thanks for sharing this video!

[@preston1382]

Thank you for being much more helpful than anyone in my lab or institution. Much appreciated 🙏

[@jackwining1928]

Lovely presentation for the flow cytometry. It really helped me a lot. Many thanks.

[@shameera4811]

Great demonstration!!! Thank you very much for the upload.

[@nadeekabandara5504]

This is an excellent demonstration

[@mariakalomoiri7403]

Amazing demonstration of FACS Diva! Thank you so much for this helpful video!

[@โสฐิดากาเหย็ม]

thank you so much for good demonstration!!! Extremely helpful!!!

[@VGliffe]

Thank you very much for the video. It definitely helped to backup what I learned. Would love to see one with compensation.

[@siavashmashhouri4969]

Great, better than our technicians :). Thanks

[@xinyiwang2956]

Thank you!!! That makes sense for the beginner!

[@kimle7049]

Thank you for such a great video! I am learning flow cytometry and this helps a lot

[@alexandroskourtesakis3673]

Very good demonstration! Extremely helpful!

[@sabrarostami4465]

Thank you very much, this is really enlightening.

[@TotodileDragon]

This is a great compensation tutorial, amazing work! I wish I would have found this sooner :'c

[@lusen5917]

clearest explanation！excellent job！ thank you very much。

[@nabilahbaby6242]

Thanks a lot! Very clearly explained...

[@karthikeyanbio]

Thanks bro. This really helped a lot.

[@lix4793]

very helpful! Indeed today I just wondered how to adjust the voltage and how to set up p2/p3/p4. Thanks a lot!

[@mermerrrrrrrrr]

very well explained...thank you so much

[@ardy4444]

Very useful and clearly explained.

[@vincenthuang6341]

Thank you VERY MUCH! Very hopeful! Please keep up the good work!

[@sAyWaHhFuL]

Yasss, you did it with this explanation! Thanks a lot!!

[@faborodeo.samuel5913]

This is fantastic work. Thank you for sharing.

[@amrishsharma457]

The best tutorial of voltage setting.Wish there were more than 2 colors though. How do you set voltage for like 3 colors. For instance, say you have PE, PerCPcy5.5 and FITC. Would you then look for setting voltage with FITC against PE and PerCPcy5.5?

[@josephkamtchumtatuene521]

Excellent video. Thank you very much! I am a beginner with Flow Cytometry and have been struggling to find someone to give clear step-by-step explanations. Question: Is there a minimum number of cells per tube that you would recommend in order to have a good experiment? Thanks!

[@khalidrashid2684]

Fantastic video. Thanks

[@kavitavats3581]

very easy explanation.Good video

[@elhamshamsaei1946]

Many thanks this was great

[@same2100]

Thanks a lot, I learnt lots of useful things.

[@Biomeducated]

You are a life saver! Thanks!

[@perikalaa]

Great Demonstration would you please add more videos on FACS and other cytometers

[@hongxiachen778]

😀Very useful. Thanks,

[@nesrinyasseralissa1735]

Great demonstration, thank youu

[@sohanaaktermina7057]

Clear explannation

[@preetibhadauria4315]

Very well explained. Thanks for the detailed information.

[@roove1537]

Thank you for this!

[@shijie3224]

It really helps. Thank you very much!!

[@danielryan855]

Very clear video with great detail. Do you have a video of the setup of a more complex experiment? One that would require compensation.

[@sintayehufekadu5979]

Thank you very much. It was very understandable video.

[@daliatarantino6781]

thank you so much, this is really helpful!

[@jingzhao9300]

This is really helpful! Thank you soooo much!!

[@hba3820]

Thank you very much! that helpes a lot

[@shimaashehata4233]

Thank you so much for this video. I learned from it a lot. This is the best video so far explaining the basics of how to run samples with 2 flourophores. I am a beginner in flow cytometry and I got a lot of info from this video. I have a question, I found that some people do a dot plot for FSC-A Vs FSC-H dot plot, is this plot gives the same info as FSC Vs SC dot plot regarding giving info about doublet and singlet cells?

[@Biomeducated]

No, those are 2 separate plots. You start with FSC vs SSC to eliminate debris (usually below the 50K markers on both axes somewhere. Next is to draw FSC-A (X) vs FSC-H (Y): the singlets are all on the diagonal. The doublets stick out to the right on the X-axis and should not be included in your gate. You can set the same for SSC (SSC-A vs SSC-H). Some people use these consecutively to eliminate doublets both according to FSC, and subsequently according to SSC. Cheers!

[@prof1400]

Very helpful. Thank you very much.

[@M_Alatawi]

Many Thanks

[@aragawgebeyehu8304]

Thank you!!!!!

[@gus.f762]

Great job, thank you!!!!

[@victornegri7763]

Very helpful! Thank you

[@tillyfreeman5192]

Where do we get the user manual for this instrument?

[@caomoyulong]

Very helpful! Thanks!!

[@arasharjomand7970]

Great work,, cheers

[@issamel9036]

Thankyou. This was super helpful ;)

[@ahussbinwali]

Thanks a lot,. very helpful!

[@reallegend1]

so nice explanation , thanks

[@tillyfreeman5192]

Hi, may I know the name and background of the presenter? Thank you. This video is so valuable!

[@ruihuama8157]

Thank you, very helpful

[@samarjitmaharana3484]

I have one doubt. Is it needed to link the experiment to any of lysed wash or lysed no wash parameters everytime when ever you are doing compensation with single tubes each colour.

[@Dezzy7model]

This was helpful. Thanks a lot :)

[@edileneoliveira991]

Great! Thank you!

[@monspark2317]

Thank you

[@jyotsnakhithani2241]

Thank you so much

[@kimle7049]

When you ran the compensation, did you run Low or High? And also for the cells? Thanks

[@hedgefield]

THANK YOU

[@justojosecortesquijano1274]

con conocimiento TECNOLOGÍA CONSTRUCCIÓN en el proceso constructivo en la resoluciones de NRS 2010

[@redtails]

My institution offers a 2 hour tutorial for what you're showing in half an hour here

[@justojosecortesquijano1274]

justo jose cortes quijano tecnología construcción ingresado del sena con tarjeta del copnia

[@SajadAhmad-bi1fp]

video quality is extremely poor so sad

[@Sama-sf4sy]

Thank you so much. It's very useful!

[@सविन-झ4झ]

Thank you so much

[@mkaiyu]

Very helpful! Thanks so much!