Sanger sequencing

This is an animated video on DNA sequencing by Sanger's method.
Sanger sequencing method is based the use of dideoxy nucleotides in the DNA polymerizing reaction. The hydroxyl group attached at the 3' end plays a very important role in DNA polymerization reaction
during DNA polymerization, There is a reaction between 3'OH group and the phosphate of the new dNTP to be added.
in 1977, Frederick Sanger had a brilliant idea. if the 3'OH group of the nucleotide is absent, then there wont be addition of new nucleotides and the reaction would stop. The incorporation of dideoxy nucleotide and termination of polymerization reaction is a random process. hence the overall mixture will have several fragements that have experienced the chain termination. If the length of each fragment is known then the location of nucleotides in the DNA can be estimated.

[@ericeckenroth9252]

Classic example of some random youtube dude better explaining a concept than my professor can

[@niyatiishroff]

funny how my professor used the video of this random dude to explain it to us

[@kimanhnguyen7160]

So do I 🤣

[@yashlandge2708]

You are from which country and which college ,friend

[@just_jagadhish5336]

True af😂

[@kaditra8695]

God bless you you're right dude...

[@1Dann123]

Just because you have a PhD in something, DOES NOT mean you are a good teacher. Thank you so much for this my friend.

[@ginsnow1780]

this is the best video on Sanger's Method I've seen. I finally understand it now. Thank you so much!

[@mightiestmouse8833]

Me too!

[@alexcracker]

i have done it at least 1000 times and i wouldn't be able to explain it better

[@mugdhadeshmukh8037]

Yes! Totally Agree! Simple and Comprehensive!

[@FLAGS4k]

Yeah, simple best

[@saboo_tage]

My goodness, people really weren't joking when they said you're an amazing teacher. Aside from having my mind blown from how genius this technique is, my mind is also blown from how amazing of a teacher you are. Thank you ever so much!!!

[@ghitabencheikh2747]

i just want to thank you, you explained this better than most of my professors during my pharmacy school years. thank you!

[@TakeitSleeezy69]

Its crazy how things like this when taught correctly are so easy to comprehend. Professors/Teachers need to take notes from this guy!

[@zulucharlie5244]

That is 100% true. The video animation and a carefully thought-out presentation enables a good teacher to reach millions of students on KZbin.

[@ChlorRene]

crazy a 6 minute video is more helpful that 1.5 hr lecture...

[@m.m.3552]

To be a good teacher, you also need to know how to talk. This guy failed at that, his english is so poor that you do not even know what he is saying.

[@rafikraidieh5536]

tf ur talking about his english is great@@m.m.3552

[@1423.]

@@m.m.3552 That's a you problem. His English is fine and clear.

[@varshakashyap6415]

Best video on KZbin available for sanger sequencing.

[@LittleMissLonely13]

This is hands down the BEST Sanger's Method video I've seen. So clear. Excellent work! Thank you

[@hillarymorin2550]

I have been struggling to understand this concept, and this video was perfect!!! Thank you very much.

[@dannaeparry5960]

The most clear and thorough explanation of Sanger Sequencing. I couldn’t wrap my head around how the sequence of the strands are determined but it is thoroughly explained in this video

[@Atj235]

I mean my professor made this topic so hard that I thought I don't have the brain cells to understand this. Tysm buddy

[@momo____15]

My Microbiology exams are approaching and this video is a pure Bliss to me.This video is perfectly making my bases strong along with making me understand the whole topic which my professor failed to during a whole 1 hr lecture in my native language.

[@CarolynKitchens-y2u]

Make sure you realize that the sequence provided in the answer is the complementary sequence and not the parent sequence.

[@crachhit6]

Saved my life, i hate not understanding something but thankfully i found this video. Great explanation!

[@wyytoxic1793]

this 6 minute tutorial saved me a full hour of time of watching my lectures, thank you.

[@snejutibarua1310]

This is called a perfect vedio. Just amazing ♥️ I'm really amazed how someone can make us understood this hard topic so easily within only 7 minutes

[@favourachimba535]

This is one of the best science concept explanation videos I've seen on youtube in a while. Thank you for explaining this concept interestingly.

[@khush0726]

U just killed it ...rather than watching so lengthy and complex videos ..its far better and easy way to learn :-)

[@christianluensmann9216]

Vey surprised that I completely understood this on my first watch through. Such a great video, thank you so much.

[@peguinpop]

This is my go to video to brush up on Sanger's method. Thank you SO much for this!!

[@jamalinoor2411]

your explanation is perfect thank you so much

[@khushnoodusta7635]

That was amazingly Explained in a very simple manner .Thanks Dude.

[@DavidRBrooks73]

Thank you. Have been going nuts trying to understand my uni lecture and other you tube videos until I found this one.

[@ThePsychokazik]

that was life changing! I hope I will use all that information on my test tommorow. I want to say hi to my dear friend Dawid

[@ktowietenwie9107]

I also want to thank you because i and my dearest friend Szymon will kick some asses on tomorrow exam

[@wesam4470]

Clear, helpful, and straight to the point presentation. Well done and Thank you!

[@DaveSmith-mv8ex]

It's insane this works for whole genomes! Excellent explanation!

[@Conscious_Sea_Magic_Wave]

So far, this is the best video explaining this topic!

[@aminaelhannaoui7523]

had to watch it twice but this concept finally clicked for me, thank you!

[@avetikkhachatryan1142]

The most amazing explanation! Simple and perfect! Thanks

[@abdullahijamiu8617]

Very explicit! I wish I could like this video a million times. Thank you so much!

[@obscure999_]

Easily the best explanation of Sanger's method. Thumbs up

[@KaitokiNohara]

Thank u soo much ..teachers will hurry to finish their chapters and nothin would enter my brain..thanks for this video

[@ryebreadisepic]

I owe you my firstborn child and my left testicle. Thank you for your services I am now enlightened

[@sorayachquiry9147]

so we're all here bc our professors can't teach anything during lecture :')

[@shriya1163]

Trueee😅

[@carolynnemtuma346]

😂

[@musiclover81813]

THis makes a confusing mess out of this. Again Shomu has the best explanation on the web. This just makes something that should be simple into a mess which is something that biologists are very good at.

[@alisharifi9547]

This is really the beeeeest video about sequencing method . very simple, very clear, wonderful!

[@romeolhk1008]

Hey you deserve a Nobel in education!

[@mine8747]

Wow I have my biochem exams in 5 hours and this is a life saver

[@watermelon9801]

Your video helped a lot hope you are continuing making this kinds of videos

[@thamodiliyanage2126]

You just saved my examination. Thank you so very much dude! ❤️❤️

[@marlenagwardys9716]

i love this video, my teacher spent 1,5 hour explaining this and i couldn't get it, now I finaly understant

[@qaisnaik1785]

Explained so nicely, and the technique itself is so brilliant ❤

[@ka1458]

YOU ARE THE BEST!! thankkk yoooouu vary vary much from deep inside for this amazing video.

[@chinnupalivela]

Never understood this except for now..... Amazing !!

[@daaiibrahim1652]

This was amazing, especially for My MCAT prep !!! cleared up so much , thank you

[@luciawang1805]

way better than my prof explaining... thanks so much

[@agneschan24]

Very clear and easy for understanding! Thank you so much!

[@lucasvantin146]

You explained this better than my teacher. Really nice video

[@awhb5412]

Very nice, simplified way of explaining. Very easy to understand. Thanks

[@seanyhotdog3125]

This was so very well explained. Thank you!

[@widushimarasinghe7133]

Thank you very much!!! The best explanation for Sanger sequencing💌

[@thomasding5574]

Exactly what i was looking for. Thank you!

[@annienguyen7439]

thank you so much for a clear and detailed explaination! Your work is much appreaciated!!!

[@kad.k1]

an absolute true legend hands down.

[@r1hasanain848]

You are the best explainer ever ❤❤

[@medtorque4998]

Not a single line to mugup only understanding....... Thank u very much sir

[@Malik-dz5ft]

Thank you making it easier and more understanding 😌

[@Sup_keke]

Thank you. I kinda understood, but didn’t understand the difference between classical and modern method. Now i do

[@garymcleod9170]

Exceptional presentation. I can breathe again.

[@jameelamanzoorsheriff]

The best video explaining sangers method.. Goodjob👍

[@williamross5308]

Phenomenal explanation! Thank you for this video it helped a lot.

[@rehambouimad5839]

Thank you sir for saving me from failing my molecular biology exam.

[@quickbiochemistrybasics]

Happy to help

[@neoscientia7108]

What a great video. I understood everything

[@habimanaarsene]

Thank you for your well clear explanations and well animated video.

[@bhaswatiroy9489]

The best video ❤ Soo easy to understand and all the details are covered.😇

[@hamzagharbal7544]

The Greatest Video in History

[@orphica5739]

Absolutely fantastic explanation. The explanation of the x-ray film method and the evolution into the electrophoretic capillary method was unclear to me in the textbook, so this saved me a great deal of time. :)

[@sikchamomile]

Very clear explanation. Thank you

[@estinien]

Fantastic video! The information was presented in such a clear way that it really helped!

[@blakewang4070]

Easy to understand and comprehend! MUCH better than my professor...

[@gabrielphilips6980]

How is it known that this is happening? Is this visible in microscope? How do they manipulate these things? I'm assuming it's too little to pick up with tweezers. How do you get a DNA if you cant pick it up with tweezers?

[@chalitah849]

This clarification is sooooo cool!!! Big thanks

[@mattj500]

Bro my dev bio professor cannot explain this sh*t in even a remotely comprehensible manor lmao. Thanks.

[@056vatsalapandey3]

OH MY GOD!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! each point explained PPEERFECTTLLY

[@santokhsingh1696]

Thanks save maah time ....way of delievering content was fabullous

[@DavidChristmas]

Very helpful! I just had a question though, we know that DNA polymerase carries out a normal polymerization reaction as long as ddNTP's don't get in the way and terminate the reaction. Since we found the sequence to be as 5'-AAGCTTAGCC-3', wouldn't this sequence be the sequence of the newly synthesized complementary DNA strand? And therefore shouldn't the template DNA strand be 5'-GGCTAAGCTT-3'? I may be wrong, but I still wanted to ask just to make sure. Thanks in advance!

[@qaisnaik1785]

I think you are right

[@hubristicditch3975]

Thank you so much! I found this video very informative

[@Chiku_xt]

Finally i got best explaination on this topic thank you very much ☺️

[@L-uu7fq]

Now this is amazing explanation.

[@samcovenant9905]

So much Understanding God bless you

[@Sandra-qf9jw]

Such a beautiful class and good explanation, thankyou ❤

[@debbiehariette6335]

brilliant, thank you for making this video, best explanation on youtube

[@amruthamolpa6533]

Exactly what I was searching for... 😍🙏👌👌👌

[@AbdulrahmanSeddik]

That was super clear and helpful !

[@KShiv2024]

Very well explained.. thank you

[@joaomonteiro1562]

What a great video! thanks a lot!

[@turntablism3696]

Med school student here, amazing explanation, thank you!

[@sparkle3362]

Great simple explanation! thank you very much

[@andrewprice8717]

This was so clear, thank you so much!

[@alexplastow9496]

Excellent explanation

[@agussschh]

this video made it so unbelievably easy to understand, geez

[@alenapawlak6853]

Thank you! You explained it super well!

[@onuryavuz4673]

Thx a lot Mr. "I will teach you, my son".

[@corfex]

Very well explained, thank you very much!

[@sokunsan7086]

You are good at explanation. I can understand a lot from it.

[@aidah6381]

The best video of this method, it was great,,, i wish you teach chapter 5 of Emery's Elements of Medical Genetics :)

[@elibenaron]

amazing presentation. thank you for this.

[@mantidream8179]

Phenomenal explanation

[@archanag6974]

awesome explanation and visuals

[@michellewoolridge4557]

Thank you for the explanation! Easy to follow and understand! :D

[@alekharry2223]

I could learn this I my sleep, so easy to follow

[@hutshutrules123]

Thank you! Very helpful lecture and an amazing explanation as to how the procedure works.