Single-cell sequencing explained in 2 minutes

[@cold_hardfacts]

Im not a scientist. I came here from cancer research. I gave it a thumbs up. Clear and informational.

[@roshanpatel06]

Your videos are perfect. I appreciate the no-intro, straight to the meat style.

[@sagek7949]

This was helpful. Thanks

[@sanbomics]

Glad it helped!

[@szymonjakubowski3574]

Awesome! Very clear explanation, thank you

[@sanbomics]

Thank you!

[@michaelbailey9643]

Great video and summation. Surprised it doesn't have more views!

[@sanbomics]

Glad you liked it!

[@user-fo8we8ur4n]

Why is it easier to understand on what you are saying than to understand it on my language hahaha,,

[@sanbomics]

Hahah xD People just like to make it more complicated than it really is!

[@kamarsedik1448]

Could you suggest me a simple reference for beginners regarding scRNA_Seq

[@keeganhart2308]

Great brief description. Thanks!

[@sanbomics]

You're welcome!

[@jappe008]

Thanks for the video! Could you maybe elaborate on common use cases for single cell sequencing? E.g. what questions could you answer after analysis? Just curious of the bigger picture where scs fits Thanks!

[@sanbomics]

Great question! There are many potential use cases, but they all more or less boil down to one core concept: each tissue you type you examine has multiple cell types which all behave or react in their own distinct way given a given context or stimulus. Here is a quick examples, but there are many more you can extrapolate this key idea to: Is there a specific lung cell population that responds uniquely to infection?

[@neurostudywithme]

Is UMI shows nFeature or nCount in the sc rna analysis?

[@sanbomics]

typically nFeatures are the number of genes and the nCount are the number of UMI

[@neurostudywithme]

@@sanbomics thank you so much!!

[@saveriov.p.7725]

i dont get how the bead somehow tags each RNA transcript in the cell if it just sticks to the outside?

[@sanbomics]

The bead, a cell, and the reagents all go in one droplet. Then the cell gets lysed and they are all swimming together

[@ShenStefan]

@@sanbomics Does each bead contain many DNA segments with the same barcode and different UMIs?

[@sinamt2982]

Thank you so much!!

[@sanbomics]

Glad it helped!