Synthesis of Silver Nanoparticles

[@WisconsinMrsec]

Full written instructions to go with this video are in the video description.

[@syedshahab123]

Procedure: Step 1. Add 30 mL of 0.002M sodium borohydride (NaBH4) to an Erlenmeyer flask. Make sure the solution is made fresh right before the experiment. Add a magnetic stir bar and place the flask in an ice bath on a stir plate. Stir. Keeping the sodium borohydride (NaBH4) on ice will reduce the rate of decomposition during the experiment. Step 2. Drip 2 mL of 0.001M silver nitrate (AgNO3) into the stirring NaBH4 solution at approximately 1 drop per second. Stop stirring as soon as all of the AgNO3 is added. Step 3. The presence of a colloidal suspension can be detected by the reflection of a laser beam from the particles. Step 4. Transfer a small portion of the solution to a test tube. The addition of a few drops of 1.5 M sodium chloride (NaCl) solution causes the suspension to turn darker yellow, then gray as the nanoparticles aggregate. Step 5. Transfer a small portion of the solution to a test tube. The addition of a few drops of 1.5 M sodium chloride (NaCl) solution causes the suspension to turn darker yellow, then gray as the nanoparticles aggregate. Step 6. Transfer a small portion of the solution to a test tube. Add a drop of 0.3% polyvinyl pyrrolidone (PVP). PVP prevents aggregation. Addition of NaCl solution then has no effect on the color of the suspension. Step 7. Add enough solid polyvinyl alcohol (PVA) to give a 4% solution. To get the PVA to dissolve you will need to SLOWLY add it to the strirred, hot, silver colloid solution. Step 8. Silver nanoparticles color the yellow stained glass in medieval churches. To make "stained glass" decant the mixture into a mold leaving air bubbles and undissolved PVA in the beaker. 9. Evaporate in a toaster oven for 30 minutes. Alternatively the solution can be left in a hood over two days to evaporate. Materials: Stock Solutions for 8 batches 0.001M AgNO3: Dissolve 0.017 g of AgNO3 into 100 mL distilled water. This solution can be kept for later usage. 0.002M NaBH4: Dissolve 0.0189 g of NaBH4 into 250 mL distilled water. This solution must be made fresh before the experiment. 0.3% PVP solution: Dissolve 0.1 g of PVP into 33 mL distilled water. PVA solid Equipment Small Erlenmeyer flask Large dish of ice Stirrer hotplate 1" stir bar Droppers Laser pointer Mold Toaster oven or overnight drying

[@physicsacademybyzakirullah8825]

thanks dearest brother...

[@Ilovecomedy7]

What's the significance of stop stirring right away in Step 2?

[@rgacadming2367]

is these equipment available if yes then from where i get this?

[@Ibrahim920]

Assalamoalikum dear syed shahabuddin please send a research paper if you have. I am new to the field of nanoparticles.

[@Ibrahim920]

Email id is [e-mail]

[@Snizzfart]

I really would have appreciated the mass, concentrations, volume, etc. that you used.

[@mehdi4194]

all details can be found here: education.mrsec.wisc.edu/278.htm

[@shopeerereloody5732]

Elektroless chrome?

[@hohnjopkins5705]

Everyone here is mad about the lack of narration but if you listen closely and use your telekinetic abilities you can hear the persons thoughts

[@WisconsinMrsec]

While we don't make a lot of our "video lab manuals" with narration, we did forget to add a link to the written instructions! A link to the written instructions is now included in the video description. Thanks for the feedback!

[@yashikanalla299]

Can you please tell me the procedure for synthesis or the research paper

[@manupimpmaster]

I'm working on this kind of synthesis of chemical reduction reaction. You can use either PVP or PVA. Do you need to do the mixing process on a Ice water bath? or you can store it after mixing on 4 C degree? the sodium borohydride needs to be injected rapidly. This procedure is really tricky. WHY did you heat it up and add the NaCl? I don't get it at all. Where's the powder?

[@chloedominiquealgarme8903]

Can Ascorbic Acid be an alternative as reducing agent? This is our proposed process 1. Dissolve 0.1 g of silver nitrate (AgNO3) in 100 mL of deionized water in a 250 mL beaker. 2. In a separate 100 mL beaker, dissolve 0.1 g of ascorbic acid in 50 mL of deionized water. 3. Slowly add the ascorbic acid solution to the silver nitrate solution while stirring continuously with a magnetic stir bar. Keep stirring for 5-10 minutes to ensure complete mixing. 4. Place the beaker containing the reaction mixture on an ice bath and let it cool for 15-20 minutes to allow for nanoparticle formation. 5. After 15-20 minutes, remove the beaker from the ice bath and let it sit at room temperature for 1-2 hours to ensure complete nanoparticle formation. 6. Carefully transfer the remaining solution through decantation.

[@devgowri]

You should have given some explanations in this video.. a person like me, without any science background, it's difficult to understand what's happening...

[@DrInorganick]

Maybe it's just my stomach talking, but I swear it looks like you're making little cupcakes from about 2:45 onwards

[@samanehkhodadadi2972]

can you explain these step one by one?specially with the name of material

[@hohnjopkins5705]

No

[@dharmachaudhary8580]

What is the concentration in the solution?

[@zainjadran6295]

I have synthesized almost 20g silver in lab with 25g of Silver Nitrate. Can you please tell me why it gets dark grey on complete synthesis.

[@WisconsinMrsec]

Hi Zain, when sodium chloride (NaCl) is added the nanoparticles aggregate and the suspension turns cloudy gray, which might be what you are seeing. The addition of a small amount of polyvinyl pyrrolidone will prevent aggregation. You can find the full instructions here: education.mrsec.wisc.edu/synthesis-of-silver-nanoparticles-nabh4/

[@zainjadran6295]

@@WisconsinMrsecbut I have synthesized using Hydrazine Hydrate as reducing agent.

[@rabiajaved9284]

may be because silver oxidizes

[@uxleumas]

@@rabiajaved9284 Silver is already oxidized when it's in the form of silver nitrate. When silver is reduced, silver particles that are dark form. Also happens when silver is basified.

[@kremesti]

no sound/comments ?!?!?!

[@Informationcornerchannel]

can we use deionized water mixture as a solvent in green synthesis of AgNPs???...if not not then only deionized can??? plzz

[@hussaintouseef8]

hw it can b used in diagnostic of plant pathology detection ?

[@ummethahirakhatoon9910]

how much purity you got?

[@sanajaved646]

Please added full explanation without explanation we cannot understand that what are you doing

[@SubinSubairCP]

Is there any way to wash Ag NP ?

[@Ilovecomedy7]

You can centrifuge your AgNP solution , remove the top layer, and add DI water or a mixture of capping reagent at a desired concentration. You can repeat the process until you can achieve the clean level you want.

[@zainjadran6295]

@@Ilovecomedy7 but the centrifuge process cause alot of material waste as it sticks to the bottom cone of tubes.

[@750SonyP]

How not to make Ag...

[@Informationcornerchannel]

mostly i have abserved broud UV peak NEARLY 400nm...whts the reason..how can i will it sharp ??? plzz anyone

[@bdmesq]

Useless video without explanation, alas

[@egyptianprincess6006]

Where are the result of this , if u did this 10 yrs ago 🧐🤨💭🤔

[@AquariumMania]

3:19 Damn I didnt know that it was more flammable in EU than the states. xD

[@gonebamboo4116]

Awesome narrative Piss poor title

[@gonebamboo4116]

A better title might have been, motion pictures of lab activity.

[@rabbaniasamuelchaudhry7381]

boring