Targeted Proteomics Methods for Translational Biology

Center for Proteome Analysis Director and Associate Director, Drs. Amber Mosley and Emma Doud will present targeted proteomics techniques. New in 2024, the Center has begun offering the Olink(R) Explore 3072 panel. This Proximity Extension Assay based proteomics approach is optimized to analyze human biofluids including but not limited to serum, plasma, and urine. It is performed in batches of 88 samples in collaboration with the Center for Medical Genomics Next Generation Sequencing technology and provides a readout of abundance counts of specific proteins. Also discussed will be the application of Parallel Reaction Monitoring (PRM) mass spectrometry techniques for pharmacokinetic/pharmacodynamic studies of therapeutic proteins. A recent collaboration with the MODEL-AD consortium has resulted in a fit-for-purpose assay for quantification of chimeric Aducanumab in mouse cortex and plasma.