The Scanning Electron Microscope

[@DiCola119]

I'm amazed they can see the image in real time. I always imagined the image taking extraordinary long amounts of time to process.

[@walterwojcik5090]

me too

[@AnteBrkic]

probably that was true until recently?

[@johndripper]

prolly coz computers got faster and can process data faster :) now and still improving :) next thing you know you will carry a supercomputer in your wristwatch ;)

[@garethbaus5471]

The higher resolution images require a slower raster.

[@tjeanneret]

I have worked on Stereoscan 250/600/... in the 80', we have always been able to get a "television" frequency for "low" resolution. Than, when it is about to take a picture, you lower the beam current and the scanning speed.

[@sonicrising6614]

It blows my mind that somebody figured all this out.

[@garethbaus5471]

The interesting part is that someone can make a low quality one in his/her garage with only a couple thousand dollars in materials.

[@jayizzett]

So much of this vid is theory sonic. Don’t be too mind blown .. damn deceivers

[@No_OneV]

IKR

[@thatmomentwhen9718]

@@garethbaus5471 That's exactly what I'm looking for. Do you have a link to some online resource for that?

[@WhiteGeared]

@Jay Izzett Yeah I was thinking about that why sucking some electrons will produce the corresponding image? I bet there'r massive image processing going on in the background so actual image is impossible?

[@sahilraheja3538]

best explanation of electron microscope on youtube

[@MegaCadette55]

yup

[@Time2Splitt]

exactly, it's not just some shapes from powerpoint thrown together to represent what's going on... you actually get to see the experiment being conducted/hardware being used - from start to finish. If only explanations of concepts were as pedagogically considered & realised like this, a lot of natural-physical science would be easy to understand, then you science literacy wouldn't be as difficult.

[@coreycox2345]

I thought the same Time2Split. This could have been a dull presentation where my mind would wander. This was much better.

[@umamaheewaripolinati6752]

Good explanation,thank u

[@devkumar-rs8ys]

@@umamaheewaripolinati6752 so is there x rays detection

[@leopardtiger1022]

Clear very good explanation. Thank you. I used SEM in 1969 at RWTH Aachen for my Doktor work to study surface of iron ore after reduction with CO and H2. It revealed iron whiskers in different shapes and sizes and explained reason for abnormal swelling during reduction. Though I used it in 1969 today I understand with more clarity how SEM works from your video. Thank you.

[@MaterialsScience2000]

Answer to "Clear very good explanation. Thank you. I used SEM in 1969 at RWTH Aachen for my Doktor work to study surface of iron ore after reduction with CO and H2. It revealed iron whiskers in different shapes and sizes and explained reason for abnormal swelling during reduction. Though I used it in 1969 today I understand with more clarity how SEM works from your video. Thank you." Thanks for the information. The work you describe is an excellent application for SEMs.

[@johnm.v709]

@@MaterialsScience2000 Spin of Indivisible Particle : Watch... kzbin.info/www/bejne/pJ_Op6J_fd-nhtk

[@flaviamaria4956]

Xxxxx

[@ako9212]

If theres ever an apocalypse im just gonna break into a lab with one of these and play around until i get killed

[@can_uysal]

why dont you just buy one

[@Shock_Treatment]

@@can_uysal Probably because those really high-tech ones are $60,000+.

[@can_uysal]

@@Shock_Treatment probably im just joking

[@nikolausdeems1922]

SoulsOfWolves lot more than that

[@fabiankehrer3645]

@@nikolausdeems1922 Plus the Lab you have to set up

[@maker-matt]

Great video. I actually ran one quite similar back in the early 1980's while working at a semiconductor manufacturer in San Jose - on Bubb road near Deanza college. I took step coverage micrographs of mil-spec parts along with the occasional insect. Wolf spiders up close have really big fangs! and bees eyes are incredible. But you need to be very careful or you can set them on fire and really mess up the internals - takes hours to clean and re align the collumn.

[@electricruchir]

wow! that's really interesting

[@justintan7548]

It can catch fire even in a vacuum?

[@maker-matt]

@@justintan7548 Yes, as long as an oxidizer is present things will burn in either a vacuum or underwater. Ever seen underwater welding or a rocket motor in space? Just think how hot a concentrated electron beam can get...

[@edward_grabczewski]

very nice and clear presentation. The metal analysis section was a nice bonus!

[@BangMaster96]

A great explanation for people without Engineering degrees. Thank You

[@larrykent196]

Thank you for the fine job explaining the basics of how a scanning electron microscope works, including the great pictures. Cheers!

[@arnavkatepallewar1771]

The finest and exact explanation of working of an electron microscope ever!

[@thangkhiam846]

well explained, sir. easy to understand, very good resolution n very systematic operation!

[@davidlloyd3116]

I worked with an SEM as part of my microbiology degree. Brilliant piece of kit.

[@Th3Mafia]

i have waited too long to watch this video, only found it just now, great presentation of information!! thank you

[@rasmussenrambles8576]

I've seen one been used in real time It is absolutely mind blowing

[@mstepansky64]

Electron scanning microscope reminds me of a very similiar concept: like an upsided down vaccum electron tube, as in TV scanning cathode ray tube, but with more complicated controls inside to examine the specimens on the plate. Thanks to quantum mechanical physics!

[@MaterialsScience2000]

Answer to "Electron scanning microscope reminds me of a very similiar concept: like an upsided down vaccum electron tube, as in TV scanning cathode ray tube, but with more complicated controls inside to examine the specimens on the plate. Thanks to quantum mechanical physics!" Yes, there are many similarities!

[@6977warrior1]

8:46 When your nearly $1,000,000 scanning electron microscope is run by Windows 3.1, LOL.

[@Muonium1]

It's clearly Windows 2000. 3.x had up and down arrows for minimizing and maximizing windows and it had the dropdown context menu on the upper left. Anyway, the video is obviously from the 2000s.

[@mirtaet123]

Muonium From 2011

[@icesystem7]

the older the windows the more stable it is, that's what you want when running simple applications

[@epicmetod]

Doctor and scientist doesn't want update in middle of research.

[@overridezone973]

🤣

[@muffinman1157]

6:33 On the surface of the weld, it looks calm and ready.

[@applesweet55]

theres vomit on his already

[@tjd2326]

You can stop bleaching your hair now. Hes a has been. Just another mumbling left snowflake pushover lol

[@YaLokalJenkemGuy619]

Never would've imagined the microscope is moveable and magnifyable in real time. Holy shit.

[@omerufuk]

+1

[@Spirit532]

The image is much worse in realtime mode though, due to the practical and physical limitations. All SEMs have a high quality acquisition mode, which can take snapshots with good(presentable) resolution. Such scans take many seconds to complete, but you can get many megapixels out!

[@evolutionCEO]

You are happy that this is real? Looks faker than elon musks "car in space"...

[@philidor9657]

Same. I've taken at least 2 classes that taught electron microscopy but never knew you could get a real time image like that! Incredible!

[@philidor9657]

@@evolutionCEO Skepticism for the sake of skepticism isn't cute. Why do you think it's fake? Like for real, give me a reason. Because the image is too perfect?

[@carlospinto1915]

What a GREEEATTTTTT explanation!!!!! Now I'm like 300 thousand steps ahead to use SEM in my investigation!

[@NUBLAR11]

I always imagined a mad scientist in a hazmat suit using a electron microscope and here she is.

[@rajivvaidya5507]

Nice explanation. Very useful to understand whole construction and working of SEM.

[@abhishek.chakraborty]

Thanks for providing an actual hands-on explanation of SEM .... to be frank, I did have an idea of what it does, but I think now I have a clear understanding of how it works too from this video 👍

[@jvargas454]

I know this instrument. You did a very good job of this presentation. I watched the whole thing.. great.

[@dollmonn3641]

Beautifully illustrated and awesomely shown.

[@weiwang5455]

Thank you! Very education and illustrative. Very clearly explained.

[@Muuip]

Amazing technology! Great presentation!

[@jaimeruedagomez]

Great video, but what I like the most is the extensive use of comic sans.

[@breather8758]

Yeah me too! I liked the Megalovania music playing the background of the video when Comic Sans started displaying on the screen! Oh.. Someone is knocking on my door.. Well, I just realised why. I forgot to pay rent today

[@breather8758]

Nvm sorry for the confusion! I just forgot to pay for my protection money

[@RanzithDTC]

Superb what a explaination bro electron microscope costs more than a Lamborghini so we have to thank him because he made us to watch this microscope

[@BushCampingTools]

LOL, great i worked in a EM facility lab for ten years, can't get enough of them!

[@persiankingish]

J.J Thompson must be smiling from the heaven.

[@architectinth]

The non-destructive chemical composition detection due to the type of emitted x-ray is freaking brilliant. Pieces of the puzzle become illuminated once you begin to construct the picture.

[@boreliozazlymestronapacjen1062]

the stuff the people do is unbelieveable... what a beautiful technology

[@gayatriprasad3790]

Best video explanation on scanning electron microscopy.

[@Scientist_Albert_Einstein]

When I win the lottery I am going to buy one of these.

[@lbochtler]

don't forget the equipment needed to support the SEM and prepare the samples.

[@1sirgrandmastermrkingrober216]

Thank you, this is very helpful in Police work, always.

[@farrisisable]

Very informative. And what a pretty SEM user!

[@Rizwankhatri786]

It was my first time to watch a EM although I have completed my master degree in science...professor always used to say that EM cost is very high. I don't know what's the cost or price of this EM...

[@dr.rahulgupta7573]

Excellent explanation of electron microscope. Thanks a lot.DrRahul Rohtak.India

[@superman9693]

Very interesting and very beautiful operator!

[@BTS_12898]

Nice lecture on scanning electron microscopy .... thanks for ur adorable lecture .

[@585585MC]

6:52 SCIENCE IS INTERESTING

[@leehyeri9863]

I wish I could see it it in person and use it like her 😭! I just used the light microscope in my schools biology lab and It was fantastic!!!! Idk how amazing it would be to see through a microscope with the magnification of 250000 times !!!!

[@saadachab8425]

I never had such explanation of electron microscope before!

[@sarveshrane8739]

Thanks a lot !! Explained really well !! 👍

[@ouzytheoriginal]

great job, crystal clear

[@raymondamaro3082]

When I was an ojt for a semicon company. We have this kind of microscope use for product / failure analysis. I miss using this machine.

[@dr.rameshbhise2178]

Best explanation of electron microscope

[@jyotinarwal6795]

Amazing conceptual explanation and demonstration

[@brycefant9106]

“You know, this is the largest electron microscope on the eastern seaboard.”

[@ratnabesra8959]

Mind blowing clear explanation again thank you sir

[@Simonjose7258]

Wait people! Y'all don't think this is crazy!? 🤯 The electrons aren't "reflected" like photons would be... They're accelerated towards the sample which knocks off a "secondary electron" ... I don't fully understand but I did Google it and apparently they use both "backscatterd" or reflected electrons as well as secondary electrons. Apparently the simplest kind of SEM just uses secondary electrons. Supposedly they help to improve resolution in the topography of the sample. I still think it's crazy... they're not reflected, but emitted from the atoms and then pulled towards the oppositely charged detector which then measures...the intensity of that electron? 🤔 Aren't all electrons the same? Maybe it has something to do with interferometry? I did find a "Nanofabricated, monolithic, path-seperated electron interferometer" Not a bad idea apparently. 😎

[@OmarFaruk-dt3hs]

As a undergraduate student, I think it is the most easiest way to understanding the principle and procedure of SEM characterization of a sample

[@_arpheus]

Would love to see any moving organism or virus in this

[@abuahmedzahid538]

Nice & well explained. Thanks a lot.

[@rock3tcatU233]

These things are so much fun to play around with.

[@Quazi-Moto]

If you've done so, I envy you more than you can fathom !

[@robertschlesinger1342]

Excellent video. Very interesting and informative video.

[@marcelmaes5275]

"Best explanation" is what came up to me too. Obviously I'm not the only one. Never understood what this word "scanning" means in the context of electron microscopes. Many thanks to the Hochschule of Karlsruhe.

[@ManicallyMellow]

everybody gangsta until the flange is sucked off by a vacuum

[@eduardadecastroflach7745]

Congratulations! this video is quite helpful

[@defenderslamare7266]

Wow! This is truly amazing...I love it😎

[@johnmike121]

The scene from Super Troopers "enhance... Enhance.. enhance.." comes to mind 😂

[@farzadtatar3702]

This video is wonderful, Thanks for preparing and sharing it

[@parimisreekar]

Appreciate the presentation. A good overview.

[@aartijoshi9051]

Sucha!! nice explanation loved it.❤

[@EphemeralProductions]

I've always thought that how things look in an electron microscope is REALLY cool! except when you are magnifying creepy looking things like bugs and other such creatures. LOL

[@obviouslytwo4u]

3:48 He means Secrets

[@AizatZakaria]

so in general, the scanning electron microscope work just like a typical cathode ray tube (CRT) television. where the image is formed by bombarding the specimen with high velocity electron. and scanned the specimen in raster ways, just like a printer. but the intricate science behind those machine is super amazing.

[@rashmiranjannayak3251]

Excellent video and its simplified presentation, question: what is its last scale of observation.

[@MaterialsScience2000]

Answer to "Excellent video and its simplified presentation, question: what is its last scale of observation.": Thanks a lot! If I understand you correctly, you are referring to the magnification. The best way to check this is by using the length scale bar on the screen. The length of the bar is specified directly above it.

[@rashmiranjannayak3251]

@@MaterialsScience2000 Yes; about magnification , Can we measure the atomic diameter of an mater ?

[@MaterialsScience2000]

Answer to "Can we measure the atomic diameter of an mater ?": Directly no, the resolution is not good enough to see atoms. But indirectly yes, by special diffraction techniques, which is a very special topic.

[@rashmiranjannayak3251]

@@MaterialsScience2000 OooH Yes, thanks for your valuable information.

[@tsmentftx6117]

Now this is what called physics 💯

[@travisr3]

Ask me any questions. I am a senior field service engineer with Hitachi. I install, service, maintain and most importantly train my customers on how to use electron microscopes.

[@arslanahmad1195]

Travis Rice Can a true color image be generated with an electron microscope?

[@timblade7369]

Arslan. No, only black and white images are created by this type of microscope, as the electrons collected don't contain any "color" information. They can, of course be artificially colorized after, using image processing software - typically by assigning a particular color to a specific brightness level.

[@travisr3]

See Tim's reply below, he is correct. Any color that you see on an electron microscope image has had image processing after the image has been captured. In published reports, this is typically not done because scientific studies do not like alterations to an original captured image. Color is normally added for illustration purposes only, and to make it look less boring when these captured images are being shown to people in meetings, etc, because usually these people will have no idea what they are looking at.

[@arslanahmad1195]

Travis Rice Thanks

[@hussainrt3242]

Travis Rice what's the most affordable way to obtain an SEM? even if it's an old used 1980 era SEM?

[@joeferdin9871]

This video is a masterpiece!

[@Marckiller2n]

Just perfect explanation

[@michaelromano4352]

this is great. i work at a college and we have a fully computerized unit (PSEM). i am going to recommend this to the professor as i am only a technician.

[@lbochtler]

the PSEM is quite a nice little machine, depending on which version of the PSEM you have, its either the first fully computerized and computer controlled commercial SEM.

[@Harshavardhan-es5xt]

Beautiful content... Really amazing sir...

[@dareniu]

Where did the cobalt plate go? I was so excited and watched till the end :( Great video, thank you!

[@MLX1401]

In the video it is mentioned that the plate is only used for calibration :)

[@jimrutin]

Thank you for this excellent explanation!

[@huskyshikakatan]

It's really helpful with such clear illustration. Thank you!

[@Rezadehghani-d3o]

Hello thank you so much this i am sem operator i have worked 15 years

[@alhdlakhfdqw]

Thank you very very much! it was a great detailed clear explanation! i really appreciate it :)

[@siddheshshewade7063]

Very good explanation

[@alexeyl7884]

Thank you for intelligible explanation. Also I envy that ball.

[@ayeshadanish1602]

Thank you very well explained and animation 👍

[@mariana.tamura]

Nice & neat work, thanks a lot!

[@sachinmandal1703]

Best video on electron microscope and its working. Very nicely explained.

[@hrhbrightigwe]

This is a. Practical based knowledge. I. Wish you can conduct a Short Course on the use of SEM. We in developing world neeeds it. Also, I need a copy of this clip and any other. I appreciate this, please keep it up. Bright Igwe, Nigeria

[@kaxtorplose]

2014. That's when this video was made. The OS looks like Windows 98. Or Windows for Workgroups.

[@sergio.tellez]

Beautiful video, thanks.

[@kerguule]

Very informative explanation! Thank you!

[@billtull]

This video is very good, a little basic but for the time allowed, very informative, thank you!

[@WhyPhi]

How do the secondary electrons carry the image? This is super cool. Better than relying on reflection as with tradition light they can actually attract the image carrying electrons and convert them to light?

[@lbochtler]

your thinking of the reflection electron microscope. The SEM has a single point that is moved in synchronicity with a second spot on a CRT Screen. This point is modulated (changed in brightness) with a signal from a detector within the microscope. This detector (usually a Everhart Thornley detector) attracts secondary (low energy) electrons knocked out of the sample by the primary electron beam (electron probe). These secondary electrons get converted to light by the Scintillator, then measured and converted to an electrical signal via a photomultiplier tube, amplified many thousands of times and sent to the CRT mentioned earlier. In Mördern machines the CRT is replaced by a ADC and a computer screen, but the basic principle still holds. The image is then build up by scanning the electron probe across the sample

[@jayashreebagawade696]

Thanks a lot for Brief explanation of SEM.

[@shunmugapriya4107]

Wonderful explanation of SEM

[@mohamedezaldine6174]

more than amazing explanation

[@jethrobo3581]

Superb presentation!

[@marcelmaes5275]

5:30 "many recordes secondary electrons lead to bright point ... none to a black point" Clear, but how translates the structure of a specimen (which is what we want to see) to more or less emitted secondary electrons? Is it because parts of the structure are hidden to the primary electron beam, so something like shadow for sunlight?

[@marcelmaes5275]

@Ian Stevenson Okay, thank you for the clarification. Had to read it 2 times (I'm not a physicist), but it is clear now. One thing is more obvious then it was before: a SEM is a remarkable tool, both in how it works and what it reveals. *That's* what our brains are made for: not for fighting wars and denying viral outbreaks. Thanks again!

[@meenus5458]

But how exactly these secondary electrons can actually give a magnification?

[@amirshahzadshahzad3418]

Helo

[@lbochtler]

they don't. The secondary electrons give you a signal you can use to construct the image by scanning a point across the sample and simultaneously on the view screen. The magnification comes about by changing the ratio between the scan size in the electron microscope (making it smaller) while keeping the scan size the same on the view screen.

[@kuhataparunks]

extremely informative thank you very much for showing the microscopy in action

[@daltoncorriea6158]

that is a cool looking micro scope

[@corumuk67]

Very informative, clearly explained and nicely demonstrated. Only thing i' didn't get was why the secondary electrons sometimes form a light spot and sometimes a dark spot on the raster image. Is it to do with the number of secondary electrons given off for instance or maybe the angle of the material to the beam of primary electrons?

[@MaterialsScience2000]

Answer to "Is it to do with the number of secondary electrons given off for instance or maybe the angle of the material to the beam of primary electrons?" Both of it, and there are some more influences. Bright spot, when many secondary electrons are registered in the secondary electron detector, dark spot when only few electrons are registered. - Many secondary electrons are registered > at a thin part of the specimen > at an edge of the specimen > at an inclined spot of the specimen > and at a surface that is tilted towards the secondary electron detector - Few secondary electrons are registered > at a thick part of the specimen > at a flat spot of the specimen (perpendicular to the primary beam direction) > and at a surface that is tilted away from the secondary electron detector

[@travisr3]

Good ole edge effect.

[@shrishirol2206]

@@MaterialsScience2000 could you elaborate little more on this. How is the depth of the sample i.e the steps, kinks, adatoms are analysed

[@_peeyush]

Thank you for sharing this!