The ULTIMATE GUIDE to Making Tissue Culture Media

[@WesleyLeeYang]

I once went to a shop to buy a precision scale, mortar and something else which I dont remember. The clerk asked me "oh are you in culinary school?", I said "uh no, its for something else". Her demeanor suddently changed drastically and started treating me suspiciously.. I only understood the situation months later...

[@Mochi-wf3ys]

oh god

[@nolanbergeron-gasse8188]

😂 thats hilarious

[@artfx9]

The reason I always explain myself in the lab shop.

[@Alacritous]

There's an asian market near me that sells Agar Agar for 99¢ for 25 gram packets. cheaper than buying the stuff the suppliers gouge you for. and it's the same stuff.

[@HYEpower]

I use a oven, colloidal silver, my dirty room air and it works... must be the colloidal silver? Ever try that? I spray inside tissue container, bake it till it's 200 and silver has evaporated, then I add the media that has some colloidal silver mixed in.. i soak cutting in colloidal silver for 16 minutes and I also use a bleech mix separately

[@shanelair9347]

I could stand to see a video on how you do that if you make videos

[@Worldgkone]

Dear can you make video on how to start tissue culture on small scale with all equipments required and resources required

[@plantsinjars]

I have one called "How to Build a Home Tissue Culture Laboratory" that should help

[@gcdrummer02]

So did you have a specific question about prisons? What do you want to know?

[@robertfrazee232]

I have not been to prison, but I am still commenting!

[@marcfruchtman9473]

As far as I know, you pronounced cytokinins correctly. (All of my professors in the past have always pronounced it the way you did the first time, not the way that the AI voice pronounced it). Thanks for the cool video. Oh, 1 question why sucrose, and not fructose? Thanks!

[@GeraldBlack1]

"Accidentally" drop some of your hair in one of the jars and make one of those viral homunculus videos lol! I like to weigh out clay for my pottery and I always weigh my cornbread!

[@waynekiely4137]

Your original pronunciation of 'cytokinin' was the correct version.

[@sts_channel]

i want to see you clonning weed, just for science of course!

[@KeithKreider]

Thanks Laur! Aluminum foil works well and is ubiquitous, no need for turkey bags. Sometimes autoclaving stacks of more than 10 tubs at a time can cause them to collapse on themselves, even without lids Tape is also cool but you can also toss in a paper autoclave strip too

[@justinschaeffer4364]

Yay, finally people are starting to believe you can use the instant pot. I have been telling people that for years.

[@steve-adams]

I've been using mine for mycology for years, and now TC as well, and yeah. No trouble at all. My contamination rate to date is about 0.01%, and I'm about 100% sure those rare instances were because I was sloppy after the media came out of the pot.

[@plantsinjars]

I was an Instapot-denier until recently

[@timgoodin42053]

@@steve-adams Me too.

[@justinschaeffer4364]

​@@plantsinjarsI know. I watch your videos. I also use vinegar and sodium bicarbonate to adjust my pH.

[@Worldgkone]

Nice knowledge you share

[@mistrefi]

Which method is best for cannabis?

[@shannona6989]

Ask for a small scales and mention "its for use in my lab" the looks aee priceless... PH probes are great... make sure you keep the batteries fresh or they'll give incorrect readings, I use PH paper.

[@toya5031]

Can you advise on where you got the poly containers? or where can I purchase some of them please?

[@plantsinjars]

The big ones are from Plant Cell Tech. The smaller ones are from a restaurant supplier, but I don't recall the name

[@toya5031]

@@plantsinjars THANK YOU FOR YOUR RESPONSE. I AM IN THE CARIBBEAN AND IS WORKING IN TISSUE CLTURE, VERY NEW TO THE FIELD AND IS THINKING ABOUT DOING KZbin VIDEOS.

[@chinupduck4849]

sahy-tuh-KAHY-nin is valid (perhaps preferable) pronunciation. You were saying it right.

[@jeremiahmarsh6021]

I literally have the same scale. I use it when I'm baking and I've ALWAYS thought it was a druggie scale lol

[@3231guppy]

Came from another video of yours. How do you make 2% Vs 10% bleach? I'm confused...

[@skinnycartman99]

I work in plant molecular biology your original pronunciation of cytokinins is what folks in our (uk) tissue culture lab say

[@JM-ym8mm]

I once did a guide on making no-pour agar on the shroomery forum, many years ago. It was almost exactly the same as what you do here :) What a blast from the past hah, we even have the same pressure cooker. That thing cost me an arm and a leg to ship to the EU.

[@steve-adams]

"Pretty sure this scale was designed for drug dealers" I think this applies to any scale that you can't stand on

[@italoximenes8755]

I really wish the stove to have "bye" when you turn it off, just because of that "hi" when you turn it on high xD

[@79PoisonBreaker]

Hello. I have a dumb question about the sugar, how bad a Idea would honey substitute be?

[@chinupduck4849]

Anything measured in grams requires a gramscale. That is all.

[@RookieBuds]

Those drug scales are great for nerd stuff, too 🙃

[@chadhafer7781]

So cool thank you for the knowledge

[@Andrey__R]

Thank you

[@iceman7986]

awesome :)

[@theodorekorehonen]

Below

[@G-3-A-R-Z]

So you could do this to clone some olive trees for instance?

[@plantsinjars]

Yup! Pretty much any plant can be cloned in TC

[@G-3-A-R-Z]

@@plantsinjars Thanks I may have to give this a try.

[@tomocimalica7494]

You are my Queen,thanks for the transferred knowledge. 🫡