TLC in Organic Lab-Practical demonstration

Theoretical and practical demonstration for Thin Layer Chromatography (TLC)

[@shahalambhuiyan21]

Excellent explanation on TLC! This is very useful for my organic synthesis and purification. Thanks for sharing this.

[@ZoyahKhan]

Really Very Helpful. Thank you so much ☺️ Keep uploading practicals kindly....🤗

[@elizaahmed2066]

Sir my compounds are non polar compounds. so which solvent do I use for dissove the compound and what solvents do I use in the mobile phase? sir can you tell me a little bit? I am facing a lot of problems in tlc checking

[@Dr.Mosharraf]

Please watch my other videos on column techniques and separation.

[@roshanthedaylight8232]

Great job 👏

[@Dr.Mosharraf]

thank you bro

[@almche8978]

Thank you doctor.. if i did TLC for two reactants with one products . If i showed all three spots on the front of solvent, how can these spots move down...

[@Dr.Mosharraf]

What was the solvent you used?

[@almche8978]

@@Dr.Mosharraf ethyl acetate: hexane or chloroform : methanol, i donot know which is better?

[@Dr.Mosharraf]

You should watch my TLC video. kzbin.info/www/bejne/oKbceppnnrR9m68 I think you will get answer.

[@DrstrangeLifeinchina]

I have many question. Please mention me when and where I want to join your live. Please

[@Dr.Mosharraf]

Okay I will do.

[@alebraheme]

Thank you for this video.. İ have an question Yesterday i separated my sample by TLC to see its purity and it looked only two spots, two compounds but when i used column chromotography to separate these compounds and used again TLC it looked like there are three compounds and more İ can't understand what happened My column dried during louding the mobile phase, is there any relation with that?

[@Dr.Mosharraf]

Your compound is unstable in silica gel columns for longer time. Maybe it’s degrading or isomerization happening. To check the stability of your compound in silica, you can do 2D TLC. It will help you to realize the unstable conditions. Keep the compound spotted on TLC for long time then run it in the mobile phase. See if you see any difference. That is another way.

[@alebraheme]

@@Dr.Mosharraf thank you so much for your reply 🙏

[@alebraheme]

My organic compound contains keton group, i tried different types of system but both impurity and compound of interest are moving together, they are too close. Also there are other problem when they moved in the TLC layer, the impurity was on the top of the compound of interest. However, when they move in the column the compound of interest became first and the impurity become last. İn sometimes, the impurity became first then the compound of interest became second, then both of the became together, and in the last the impurity become. What the explanation of that😢 İ am new in organic chemistry field

[@Dr.Mosharraf]

I will be on live. Come to there I can explain.

[@alebraheme]

@@Dr.Mosharraf please if you will do the live can you save it, I appreciate all your efforts 🙏🙏

[@Dr.Mosharraf]

@alebraheme I will try to come to live again. Today I was around 40 minutes and since no one was interacting I didn’t talk anything. I was waiting for viewers to interact and discuss. Next time I will expect some people to discuss organic chemistry and problems.

[@alebraheme]

@@Dr.Mosharraf we have time difference Now in our country it is Monday 12 September 7:44 am Good morning 🌅

[@Dr.Mosharraf]

@alebraheme in which country you live? In USA, Wisconsin time is 11:47 pm (Night)

[@vikramphulwale6071]

this is vey helpful video. plz explain me how can we decide conc ratio directly that starting from 10% OR 1% ETHYL ACETATE OR DCM

[@Dr.Mosharraf]

Depending on your functional group present in your compound. More polar group more polar solvent.

[@seemameena8222]

Kon kon synthesis pe work kr rah ah

[@Dr.Mosharraf]

There are many different types of synthesis