Training on Image Studio Lite and Image Studio on the C-DiGit Chemiluminescent Western Blot Scanner

Todd walks through all of the aspects of Image Studio Lite and how to set up and use Image Studio on the C-DiGit Blot Scanner.
For more information, visit:
** www.licor.com/...

[@leixiao169]

List of signals of each band, click "shape" tab starting from 29 min; Export File 37 min;

[@leixiao169]

For western blot quantification, start with 18 min

[@zenildasales6862]

nossa gostei muito deste estúdio de criação bem diferente de outro que já vi

[@leixiao169]

Background set top/bottom, start from 27 min

[@leixiao169]

Auto add WB rectangle bands, start from 22 min

[@vimalramachandran]

I keep getting "infinity" values for even light bands, which is very frustrating. Any solution? I use imported JPG/PNG images acquired using a CCD camera.

[@premaagarwal3387]

exactly i am also getting the infinity value not the quantification value . Did you get any solution if yess kindly please share with me. As it seems like person from licor is not going to reply. Thank you so much in advance.

[@vimalramachandran]

@@premaagarwal3387 Hi, yes I managed to resolve the issue after a lot of frustrating trials & errors. You need to reduce the brightness of the image before opening it in Image Studio Lite. Sometimes you may also have to reduce the contrast. Just play around with those two settings until you get actual values instead of infinity.

[@premaagarwal3387]

​@@vimalramachandran ​ Thank you so much for sharing your troubleshooting with me. I will try to adjust these two settings. Also do you know any other software for such kind of analysis.

[@vimalramachandran]

@@premaagarwal3387 ImageJ. It's not that user friendly though, but still many people use it.

[@user-kr5to7ic5k]

''Size of the boxes does not matter''.. Come on ... even a singal touch on the box change the value!

[@ghannimahalghuwainem2686]

Confusing .. his voice is not with the time of what he was doing

[@Daniel-zb1cm]

Todd explanations doesn't coincide with what he's doing with his mouse cursor. Confusing!