Tutorial 5: Basics of Pymol Part 2 (measuring tool, polar contacts, mutagenesis)

[@Vaeuca]

Thank you friend. Clear and organised presentation. Good work

[@DrKteaches]

+Tharaka Wijerathne You're welcome, hope it helped!

[@mailvini]

Thank you soooo much! Exactly what I needed!!! Great job!!

[@DrKteaches]

+nerdybirdy Thanks!

[@JCjohnL]

thanks for making the video. its even informative for me as a molecular biology student.

[@DrKteaches]

+JC Liu No prob, glad you liked it.

[@arminschmidt4072]

how do you know about the exact positions of the inerface region 98,508,509? where did you get these from?

[@aditikonar2411]

Can the amino acid substitutions be done in the free version of pymol? Also can I understand at what position substitutions are not possible due to steric clash etc.?

[@ashwinnair4816]

Thanks for the great explanation buddy

[@DrKteaches]

You're very welcome!

[@shafi1837]

Thanks for uploading!!!

[@DrKteaches]

+Shafi Uddin You're welcome!

[@noahedensor9405]

how do you know if the polar contacts are hydrogen bonds or salt bridges?

[@binuantony5985]

Please talk about adding disulfide bond

[@eijazbhat6317]

Thanks for uploading this video..It was really a great informative for me as a structural biologist ..Would you Please send me more related videos.

[@rainingoutside5776]

Great job

[@---gg6dl]

Thank u very much it was really helpful. can u plz tell me how to make a mutation which is deletion to one or more residue ?

[@DrKteaches]

Fatima Amanullah I'm not sure if I understand your question. Do you mean how to delete a residue or multiple residues?

[@---gg6dl]

TheScyther88 yes, your type of mutation here you made was substitution (change amino acid with another) so im asking what if my mutation was deletion of one amino acid ?

[@DrKteaches]

Fatima Amanullah You can delete the residue by selecting it, and then under the action pull-down menu, you can choose to delete the atoms in the residue. This will leave a gap in your structure however. I know there is a way to ligate the backbone after deletion, but I do not have too much experience using that function. I can do more research on this and make a video about it later on.

[@DrKteaches]

Fatima Amanullah I uploaded a new video about amino acid deletions. Hope you find it helpful.

[@谭旭昇]

amazing！！

[@صالحعباسحمدعماره]

thanks