Ultramicrotomy: The Art of Sectioning for TEM

1. Trimming
2. Small Section
3. Large Sections
While learning how to section I found it difficult to understand the concepts without a visual aid. For my final project in the TEM course I created this video to aid other visual learners. I apologize for the poor quality but hope that this video is informative.
This video is for educational purposes only.

[@TheJdhisto]

Things are much simpler these days. There are many unnecessary steps and techniques included in this video that are not currently needed, and the ultramicrotome has advanced drastically.

[@jivkokatz]

Great video! Thanks for uploading. Music made it even more epic ;)

[@joelturner1422]

Really good explanation of the steps involved! Thank you!

[@blanca4287]

This will be great for my students! It's so hard to crowd around a microtome, but this video will help with that! :)

[@matthewc7138]

Where do you teach?

[@molkhal]

This was so satisfying to watch

[@chiaruscuro]

Excellent video. Thank you for this!

[@lisseliza]

i am appreciating the tron soundtrack

[@alphonsagrace1013]

Thank you.

[@meenakshimalhotra59]

This was so helpful thank you very much❤️

[@hassansadek3665]

thanks a lot, this video is very useful

[@reemmatrook1104]

Very useful,,,Thank you very much!

[@guangmeihuang1993]

Yes very good! Thank you!

[@robinhowes6694]

The block is far to far out of the chuck, this causes inconsistencies in section thickness .What , no Diamond Knife ? Electron microscopist with 40 years experience . Have you ever tried cutting plant material ? Very difficult !

[@danielvocelle1414]

Thanks for the feedback, this was a project in my first year of graduate school 10+ years ago and I haven't used the technology since. If there is a more helpful video tutorial now a days I'd be happy to link it in the description.

[@humanhealthcom]

Grate sir

[@dongliangli7658]

Very useful，thx!

[@pooltrader]

wow, so cool, ty

[@basisTermium]

How small is 70nm just curious

[@hectorherrera526]

Smaller than the diameter of the coronavirus. Coronavirus is approx. 100-120nm

[@junglebaby448]

what camera did you use to record this??

[@danielvocelle1414]

Just the camera on my phone

[@junglebaby448]

@@danielvocelle1414 did you just mount the phone?

[@danielvocelle1414]

@@junglebaby448 yes, built a holder out of wood to hold it on the objective

[@lotharmayring6063]

i dont think that it is nessesary to make marks for trimming becuase anyway you are not cutting on the mark

[@MarySmith-gu5nb]

I hate the soundtrack. Makes it sound like something horrible is going to happen.

[@danielvocelle1414]

Thanks Mary

[@robinhowes6694]

Block too far out of the holder. Dreadful score marks on the sections - throw that knife away ! Fit a new one or use a diamond knife

[@danielvocelle1414]

Happy to report knife was thrown away 10 years ago.

[@robinhowes6694]

I'm glad you did! I started doing ultramicrotome in 1966 using a completely manual Cambridge ultramicrotome, with glass knives, cutting phloem tissue. Very difficult! I moved to the Institute of Ophthalmology in 1971 and finally ended up using a Leica ! I retired in 2011,after seeing many changes in TEM and SEM Happy cutting !