UV Vis spectroscopy 🌟

[@EdwinEsonwune]

Nice one 👍

[@chemfunman]

Thank you! Cheers!

[@chemfunman]

NEW UPDATED UV Vis video HERE kzbin.info/www/bejne/j4mVdX6orbN9rKs SUBSCRIBE NOW to this channel now to receive more great videos! Connect with me on Twitter @chemfunman (twitter.com/chemfunman) Stay safe! “Ever tried. Ever failed. No Matter. Try again. Fail again. Fail better.” - Samuel Beckett

[@nwanduchikeluba4094]

Thank you. How should I go about it using an extracted polysaccharide as sample to test for binded protein?

[@anjalisharma314]

Thanx 👍👍👍

[@chemfunman]

Welcome 👍

[@umardrazbsche-m1428]

Excellent 👍😊

[@chemfunman]

Thank you! Cheers!

[@GauravSingh-qn8ll]

Very nice understanding concept

[@chemfunman]

hope you liked our effort, thanks!

[@WalkingAdventures0_0]

Thank you from Malaysia..your vids are really helpful :-)

[@tsoekmanyiu8580]

Nice one , maybe you can buy one to play in home 🤪

[@chemfunman]

That’s right!

[@tsoekmanyiu8580]

I did have one, but not Shimadzu The one I have is ocean insight STS series

[@tsoekmanyiu8580]

STS series much smaller than Shimadzu uv1800 The size much smaller than EZ link card for MRT

[@pushpamn7886]

Excellent video and great explaination

[@chemfunman]

Glad you liked it!

[@hhuman7107]

This video is absolutely full of informations👍👍

[@chemfunman]

Merci pour votre réponse !

[@augrsc]

Thank you very much! I can't wait to test my sample using the instrument.

[@chemfunman]

당신은 가장 환영합니다! 진심으로 감사드리며 이 영상을 다른 사람들과 공유하시길 바랍니다. 축복받은 날 앞으로!

[@bhawnajoshi7313]

Very nice explanation thanks for that

[@chemfunman]

Glad it was helpful!

[@frankreiserm.s.8039]

Great video. you have a smothing voice, and it is quite pleasant.

[@shahaarifulislam6927]

Very Nice demonstration,

[@chemfunman]

Thanks for your support! Share the good stuff.

[@amanaparbin4390]

Thank you so much ❤️

[@chemfunman]

You’re welcome 😊

[@shaikmerajaaquib5344]

It would b a great help if you can explain to interprete the data from calibration curve (especially for the estimation of Ammonia in water). Thank you

[@chemfunman]

Let me work on that!

[@safdarali8740]

ooooh wavo it was amazing demonstration

[@AdnanAfzaal22]

great working

[@chemfunman]

Nooice

[@peiyafoo8607]

thank you so much for the explanation! 🌹

[@chemfunman]

You're so welcome!

[@MariiaMikhailova]

Спасибо!

[@chemfunman]

welcome!

[@paulynnesantos3595]

Sana all pwede makagamit ng magandang UV vis

[@anoopaannthomas4555]

This is very helpful Thank you

[@muhammadakif9039]

Very nice.

[@chemfunman]

Thank you! Cheers!

[@saniaparveen9428]

Awwsomee...very well explained✌👌

[@chemfunman]

thank you! you are welcome - come back to this channel for more! like and subscribe :D

[@poudaiahambika2355]

Very helpful thank you 😊

[@chemfunman]

Glad it was helpful!

[@taimazaman2089]

Very helpful vdo, thanks

[@chemfunman]

Glad it was helpful!

[@khandhanish6154]

Very good

[@chemfunman]

thanks!!

[@sitianisahnurfajriyati9928]

This is helping, thank you🙏

[@chemfunman]

You’re welcome 😊

[@shaistamurk2142]

Thank you soo much really helped me

[@chemfunman]

You're welcome!

[@monique1005]

Your channel has been SO helpful to improve my lab skills!! Thank you so much

[@chemfunman]

I'm so glad! You are most welcome!

[@uhoh5473]

@@chemfunman when is says maximum absorption wavelength for compound does that mean if you shine a light at that wavelength it absorbs it the most 8:38

[@chemfunman]

this is true given the range of wavelength, so from this standpoint, it is right@@uhoh5473

[@riyamaurya6129]

Very helpful one😊

[@sebastinraj440]

Thank you very much sir

[@suparnaroychoudhury2831]

Great😊

[@chemfunman]

Thank you! Cheers!

[@pranavkumar09]

Thank you for the explanation . I have now literally understood the process ..... ✌🏼💜

[@chemfunman]

You're so welcome!

[@panzerop8972]

Outstanding

[@chemfunman]

It's my pleasure!

[@basmaaboalazm1805]

thank u soo much for this great video

[@rianaaja144]

should we get the curve calibration and lambda maximum first before testing the sampel? or does it not apply to double beam spectrophotometer?

[@chemfunman]

Yes you are right

[@rianaaja144]

@@chemfunman thanks for answering, didn't expect it because this video is 7+ years ago😤

[@chemfunman]

@@rianaaja144 still valid today yea?

[@nataszaadamus2266]

So helpful, thank you

[@chemfunman]

You're so welcome!

[@eng.safawy5510]

Thank you very much for your useful explanation

[@chemfunman]

Eng. safawy you are most welcome!

[@chandrasekharm8628]

Sir you expline tha animation are also very helpful

[@gowthamir8562]

Thank you so much, the video is quite clear and understandable.

[@chemfunman]

You are welcome!

[@harithalakshmi1348]

what reference you use for carbon quantum dots for this study

[@yenloongleanfop6942]

I am using your video for online practical class during this lockdown period. It is very useful. Thanks a lot.

[@chemfunman]

You are most welcome. Feel free to use and share. Perhaps the best way is to have students to scaffold their thoughts in this platform. I find that it worked for my students as they spark ideas and clarify concepts

[@kanapathysima9550]

love this video..i too using uv vis 1800...this will be very helpful to me..tq very much..

[@SaadSaad-mb4py]

Thank you very much

[@chemfunman]

You are welcome

[@karasalasyamnagaraju5964]

Thank you I understood uv operation

[@merveilkatembo.m7737]

Tkank you fot this test

[@chemfunman]

thank you for your support! merci! do subscribe and share our videos with your friends :-)

[@SaadSaad-mb4py]

Good very good

[@chemfunman]

Thanks

[@mohammadhabibullah7453]

Hi . I am telling from Bangladesh.I read in class 12 in St.joseph higher secondary school. You are very helpful

[@chemfunman]

thanks!

[@maryamkhalid7481]

Amazing

[@chemfunman]

Thank you! Cheers!

[@iamgulis]

thank you so much , this has been very useful for my pratical exam ...

[@chemfunman]

You are most welcome

[@seemamourya3972]

Thanks

[@chemfunman]

No problem

[@drolugbengaadetitunTV]

Very good demonstration. I guess different cuvettes are needed for UV and visible light measurements

[@chemfunman]

Yes, definitely!

[@xalerforest8123]

Thank you so much . This has been very useful for my practical exam tomorrow

[@chemfunman]

thank you! you are kind, do share these videos and subscribe.

[@eliasalex5123]

spectrophotometry is very important in chemistry

[@sumitm8904]

Is it necessary to use liquid samples? Can I use gel samples instead of liquid samples?

[@chemfunman]

tough as it will conglomerate

[@chemfunman]

causing diffracted rays - inaccurate detections

[@jodiabrownblackellar8926]

Can this method be use to identify different rum ?

[@chemfunman]

it is possible by checking the wavelength of the different rums, give it a try please

[@pranalee438]

Thanks.

[@chemfunman]

You're welcome

[@tiptop9443]

Thank u so much

[@chemfunman]

Most welcome 😊

[@sivakumarreddy6862]

If we want to take reading at different temperatures, is it possible with UV-Vis 800?

[@chemfunman]

Looks likely

[@dasomoh6549]

Thank you , it is helpful !!

[@prakashjadhav6129]

Informative

[@شجاع-ز8و]

👍👍👍

[@chemfunman]

Awesome, keep watching, and sharing!

[@오레오-s7q]

감사합니다^^! thanks♥

[@chemfunman]

If I am not mistaken, the korean Han-gul is read as kam-sa-ha-me-da? ^^

[@pranavkumar09]

@@chemfunman Sir this is read as gamsahamnida which means Thank You ( in formal manner )

[@welfarecoachingacademy874]

Love you sir

[@chemfunman]

Awesome, keep watching, and sharing!

[@Susan-nk9eq]

Thank you, it is very helpful! :)

[@poragpori1226]

Please help me,why we use it in Laboratory?

[@poragpori1226]

Actually what do we measure from this machine?

[@ObakengGaleboe]

can I use this spectrometer to measure transmittance on protein film

[@chemfunman]

give it a try if this protein dissolves in the solvent

[@chemfunman]

thank you very much! please subscribe and share this video with your friends!

[@nattymonta7003]

Thank you

[@snspriya98]

Thanks ...It really helped me..I learned from this video how to operate it...I studied theoretically ...So this practical demonstration was helped me a lot..Thank you so much

[@chemfunman]

most welcome!

[@yakup.yilmaztekin]

Thank you :)

[@chemfunman]

thank you so much! do Subscribe and share this with your friends! :D

[@ThiNKBiologyThiNKVISION]

⭐Amazing demonstration video sir!👌 If you don't mind, Can i use your demo video to explain in my native language please with proper credits to you? thank you for this awesome video sir!😊

[@chemfunman]

Thanks for your suggestion. Can you share more about how you plan to do this translation? email me at fun.man [at] u.nus.edu

[@pharmasolutionpakistan7668]

Good

[@chemfunman]

That's so awesome to hear!! Do share this with others :D

[@jameswester1067]

Thanks man

[@chemfunman]

You're welcome!

[@ZunairaFatimaWUM-BCH--]

Sir plzz tell me..." For UV/Visible spectrophotometric graph the protein that is taken is first diluted??...mean first we take the stock solution of protein and then diluted it and then placed in spectrophotometer for measuring the absorbance ( for example at 280 nm)...

[@SeifEddineB]

Thank you very much.

[@chemfunman]

+Seif Eddine B You are most welcome.

[@hemakamalja2019]

good understanding us

[@Talex0210]

Thank you for your explanation

[@chemfunman]

+Alex Tientcheu you are most welcome, take a look at other videos if you find them useful.

[@Sahibchess12]

we want such kind of spectrophotometers in our university

[@chemfunman]

yay

[@harshgahlot3304]

wow wonderfully explained thanks much

[@chemfunman]

Best thing I hear today. Glad to know! bless you

[@cfc730]

Just see the video when i'm scrolling. Thank you for such a video. I have a question. Do we need COD digester for this type of spectrophotometer? Suppose my sample is palm oil mill effluent(POME). how can I make my sample to do this test? like just take the sample and measure or I need to do other thing? Can I use Hannah instrument vials to do COD test in this spectrophotometer? I know my questions are like noob but I am seriously noob in this field. Thanks again for your time Sir.

[@chemfunman]

You can use the referred instrument, it is possible to do the testing. But not the sensitivity,

[@cfc730]

@@chemfunman thank you prof.

[@numanahmed9300]

good

[@chemfunman]

Super

[@drolugbengaadetitunTV]

Why does absorbance reading go up and come down sometimes?

[@chemfunman]

fluctuations - it is still detecting. there's a margin of error.

[@drolugbengaadetitunTV]

@@chemfunman What I mean is that at 223 day 1 it reads 0.455. day 2 its 0.321 while day 3 its 0.534. What's wrong?

[@vgyan4u525]

Thankyou

[@kanapathysima9550]

What happen if I missed to autozero? Is my data obtained without autozero before analysis still acceptable?

[@chemfunman]

Yes

[@erkanyashar5135]

👍🏻

[@chemfunman]

(Y)

[@sarakulsoom401]

Why does uv spectrophotometer give negative readings?

[@chemfunman]

Strange! Did your reset the baseline?

[@labfisikaeksperimentallabfis20]

i have any question, what are different between autozero and basecorection? we usualy using basecorection for spectrum, autozero for photometric.

[@chemfunman]

If this situation arises , it can be corrected by performing an autozero operation (press the “Autozero” button.

[@ad7my90]

I appreciated tha thanks Mr

[@rssingh2734]

How can I take digital data from the instrument? Can one take data directly from instrument to plot in origin or excel

[@chemfunman]

download excel sheet, or print out.

[@chengmanxin3954]

alraight,thank you

[@shefinmytheen5450]

❤️❤️❤️

[@prefer_not_to_say]

Hi Dr, What is the difference between baseline and autozero. When you want to determine lambda max of a solution (unknown), as the first step keeping only solvent in both of the slots should we go for autozero or baseline ? Thanks in advance!

[@chemfunman]

Do autozero after you have set the baseline. The baseline is set when you have a standard you wish to use as a reference value, say, a solvent. Adding a solute incurs matrix effect.

[@prefer_not_to_say]

@@chemfunman It worked. Thanks a ton Dr Fun Man Fung.!

[@RoseMccquienly-r9g]

Hello, may I ask where to download the software for this UV?

[@mp3huntertr]

can i detect components of mixture and anylze concentration of inorganic liquids?

[@chemfunman]

yes - you need to make sure you have a standard curve

[@mp3huntertr]

@@chemfunman i want to analyze concentration of caustic,sodium aluminate and silicate solution.does uv fits my problem?

[@sitianisahnurfajriyati9928]

What is the merk of the spectrofotomer? Is that shimadzu 1800?

[@chemfunman]

yes it looks like it

[@KAMARPOJOK-]

Is it possible to use UV-Vis to find out the color difference on wood that staining by fungi?

[@sookl7225]

I don't think you can do that using this type of uv vis spectrometer. The sample has to be transparent to light. For solid samples you need uv vis DRS (diffusion reflectance spectroscopy)

[@chemfunman]

thanks YH!

[@Krishna_holic_1]

Nice sir please make learing video about LCMS Cromategraphy

[@intansari5983]

HELLO, can i use this method to analyze d-limonene content in fermented solution?

[@chemfunman]

check absoprtion wavelength

[@roviprog2866]

Finally a non-Hindi accent video :D (Thank God), Very good explanation, very practical, to the point and easy to understand. THANK YOU!!!

[@chemfunman]

YOU ARE MOST WELCOME!! thanks for your support.

[@kanapathysima9550]

hi i use this technique and measured the absorbance of my sample but i am getting 4.00 at 303.5,272.5 and 228nm...this sample is analysed using hplc at 280nm...the max abs is 4.0...do i need to dilute spl and scan again?

[@chemfunman]

I don't think this is needed.

[@nursuhailabintisudarman9840]

Hi Sir. May i ask how to quantify simultaneously of two drugs using this UV-Vis?

[@chemfunman]

try using GC or CE