Validating Protein Interactions with co-IP Using Endogenous and Tagged Protein Models

Immunoprecipitation (IP) leverages the specificity of an antibody to enrich proteins in a sample and investigate its interactions with other macromolecules. This presentation includes a general workflow for co-immunoprecipitation (co-IP) and what you should consider before setting up your experiment, including:
- Choice of model system;
- Choice of cell lysis buffers and when to include a pre-clear step;
- Primary antibody dilution and choice of beads;
- How to mitigate IgG masking by heavy and light chains;
- Using epitope tags for IP: advantages and key optimization considerations;
- Controls for IP experiments targeting post-translational modifications.
For more expert guidance on setting up your IP experiment, watch this Tech Tips video: How to Select an Antibody for Immunoprecipitation kzbin.info/www/bejne/rYCkkIhsbdJ9na8
For technical support to get your experiments working, visit cellsignal.com/support
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