VDJ recombination overview | Generation of antibody diversity | Antibody diversity mechanism | VDJ

[@Science_afterhours]

I'd like to thank you for this - I'm doing a MSc in Immunology and the textbook explains this process poorly in my opinion. So have saved me hours of confusion.

[@animatedbiologywitharpan]

Great to hear that you found it useful

[@kamnagupta2234]

This helped so much! Current med student in the US-thanks!

[@animatedbiologywitharpan]

Please share my channel link with your classmates and help ke to reach big audience

[@animatedbiologywitharpan]

Dont forget to check out the immunology playlist...most important topics are covered via animation

[@Lets-give-a-try]

Complete information in just 11 minutes❤❤❤

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@tioadekola-ojo363]

Very informative video. Was able to better understand antibody diversity for my upcoming exam tomorrow, thank you!

[@animatedbiologywitharpan]

quickly share with all of your friends

[@aminajoliedaou9844]

🥹🥹thank you for your explication you saved my life ❤️❤️it’s so much clear right now in my head

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@milocrawford5506]

Thank you man, for real, I was having a breakdown hear because I couldn't understand shit about VDJ lol

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@aminajoliedaou9844]

With pleasure 🥰

[@animatedbiologywitharpan]

Thanks a lot. Love from India

[@carlettagoodrich-mann1377]

Madame Curio ‘s protocols for genomes. Curing the immune system. Matters.

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@rashmiranjansahoo3975]

I have read that human contains 44 variable gene segments on heavy chain and u say 57.Can u justify it.

[@nithya_2003]

Thank youuu!! 😊

[@animatedbiologywitharpan]

could you please share my channel link with your friends ?

[@nithya_2003]

@@animatedbiologywitharpan Sure!

[@dogbeygodwin7519]

well explained but please take note that B cells mature in the bone marrow

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audiance

[@matteobarca1050]

Thank you, I finally understood it. Just a question, when you say there is a Flexibility that generate a lot of variability, you mean there are several points in which RAG1and2 could operate? Thanks a lot and a big hug from Italy

[@animatedbiologywitharpan]

Matteo Barca good question I would make a separate video for that....stay tuned and please share my channel link with your friends

[@matteobarca1050]

It's a easy question about this video, It's like a yes or no

[@sanshi7]

@@matteobarca1050 "Junctional diversity occurs because the RAG1/2 recombinase makes a covalent hairpin at the coding end. Because the hairpin can be opened at any location near its end, this can result in a random loss of a small number of nucleotides at the coding end, or the gain of a few base pairs from the opposite strand that is now covalently linked to the coding end. These latter nucleotides are called “P” nucleotides because they are palindromic to the coding end. In addition, tremendous diversity is added to the junctions by the nontemplated addition of nucleotides to the coding ends by terminal deoxynucleotidyl transferase". from - Molecular Biology of B Cells (Second Edition), 2015. I hope this helps.

[@tanmoysen7368]

Dada, if you do videos on specific section and make playlist of those videos just like you had done on CELL SIGNALLING that will be very helpful for us inspite of overview videos ....specially for people like me ( did not get much help from teachers)... I hope you will look into this request ....and keep doing amazing videos like this ...

[@animatedbiologywitharpan]

I will upload more over time....but I think my overviews are pretty detailed as well

[@animatedbiologywitharpan]

Share among friends and also you can suggest your topic...I would upload sometimes if I can

[@tanmoysen7368]

Hya dada ,my friends thoroughly follow you including me. You are our inspiration. As We are in final year of BSc biotech it will be very helpful if you upload videos related to RDT

[@ArthySuresh]

Thank you, very well explained:)

[@animatedbiologywitharpan]

please don't forget to checkout entire immunology playlist kzbin.info/aero/PLKtiwIJ8Q7rq77W7r4cXPrbhi_VpLWw8c&si=-mC4nHbVhiflO4_F

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@cck09]

Great 👌☺️👍

[@alperenaslan9332]

awesome. Hello from turkey

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@harshithamohan4074]

Thank you.... Plz make a video on Ramachandran plot...

[@animatedbiologywitharpan]

It would be uploaded soon

[@animatedbiologywitharpan]

Stay tuned

[@debabratalaik9296]

Amaaaazing

[@animatedbiologywitharpan]

Share among your friends and help me to reach big audience

[@Freak.pharma1]

Thank you so much its very helpful

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@Freak.pharma1]

@@animatedbiologywitharpan ok my friend 👌👋👋

[@Vendzor]

Arpan you are a @#$£%!*-ing legend man!!!!!!!!

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@jayanthijayaraman1995]

Excellent presentation will be helpful if heavy chain gene rearrangement is presented in Vol. II

[@animatedbiologywitharpan]

Yup I would do a detailed video on that (starting with the work of Susumu Tonegawa)

[@michaeljulius7938]

Very concise

[@animatedbiologywitharpan]

Please share my channel link with your med school friends

[@noah-battlegroundsmobileindia]

At 10:32 total Vh gene segment mentioned is 51. Isnt that 40? 40x27x6=6480. Or what is the source of this information?

[@animatedbiologywitharpan]

It was thought to be 40 but latest reference says 51 ( kuby immunology 7th ed)

[@noah-battlegroundsmobileindia]

@@animatedbiologywitharpan okay. Thank you so much

[@animatedbiologywitharpan]

@@noah-battlegroundsmobileindia please share my channel link with your friends and help me to reach big audiance

[@charliebrett7510]

Hey! where exactly does this take process take place with respect to chromosomes. I get it takes place in developing B cells. Also for context we get 23 chromosomes from each parent and VDJ happens at specific chromosomal loci AND chromosomal DNA is double stranded. So my question is does this process take place on one chromosome pair or just on a singular chromosome? Also must these chromosomes be replicated prior to VDJ recombination? Thanks so much

[@animatedbiologywitharpan]

The immunoglobulin heavy locus on chromosome 14, containing the gene segments for the immunoglobulin heavy chain. So its happening in chr 14 in human.

[@charliebrett7510]

@@animatedbiologywitharpan Thanks so much for the reply but could you please clarify whether this is on a single chromosome 14 locus (one copy) or pair of chromosomal 14 loci?

[@angelsvasquez8353]

@@charliebrett7510maybe you can find it in Internet

[@bivasnag6810]

greatly explained but so u have any method to remember this terminology

[@animatedbiologywitharpan]

Bivas Nag ups I don’t have tricks for this

[@AdityaRaj-lx6sx]

thanks sir

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@samarthkumar5198]

In which segment of Variable region CDR's are present?

[@animatedbiologywitharpan]

Near the N terminus

[@couunderbarz]

Thanks!

[@animatedbiologywitharpan]

Please checkout our entire immunology playlist. Please share my channel link with your friends

[@animatedbiologywitharpan]

Immunology playlist link kzbin.info/aero/PLKtiwIJ8Q7rq77W7r4cXPrbhi_VpLWw8c&si=KogBYNwNYcG-lV8p

[@NicoleRothstein-d8k]

thank you!!!

[@animatedbiologywitharpan]

You're welcome! Please share this with your friends who are studying immunology.

[@pragatidubey8616]

Can you please provide notes of this topic

[@animatedbiologywitharpan]

This is based on the Kuby immunology 6th ed.

[@michaelh8854]

still havent explained how you can billions of different alleles.

[@belgacem_mehdi]

Can we say that these combinations are random

[@animatedbiologywitharpan]

Yes they are randomized.

[@debabratalaik9296]

But which book you are following for b cell signalling ??

[@animatedbiologywitharpan]

kzbin.info/www/bejne/d6qVaZ-VftOXZrM

[@1100241540]

VDJ mechanism is poorly explained here..it does cover the background..but according to the title it should be focusing more on VDJ recombination itself

[@animatedbiologywitharpan]

Thanks for the criticism.... it's just an birds eye view....eventually I will summarize Tonegawa's work on VDJ recombination

[@1100241540]

@@animatedbiologywitharpan only great people take criticism positively. Wish you all the best and just to clarify, I do enjoy your videos

[@cassiecatacalos1267]

I'm giving an immunology presentation this week to a class of about 10 people. Do I have your permission to use some of your images in it? I'd give you appropriate credit :)

[@animatedbiologywitharpan]

Sure but please cite my channel link

[@cassiecatacalos1267]

@@animatedbiologywitharpan you've got it! Thank you

[@DorothyAldrich-r5t]

Aurelie Flat

[@BrayOliver-t7e]

64707 Orpha Pass