western blot analysis with imageJ

[@dragon123tt9]

I was at first confused why there was no vocal explanation, but this video was more helpful than the majority of what I could find online

[@yousufali_]

Glad that you found it helpful

[@KiaraV281]

Thank you, it was really helpful

[@AnOdeToHeartbreak]

How did you put rectangular boxes on lane 2 3 and 4? I am not able to put it

[@yousufali_28]

After you put the rectangular box on the first lane, click analyze > gels> select first lane. then you just need to drag the rectangular box and put on the second lane. So when you click analyze > gels> select first lane, under the hood, it copies the first rectangular box. You need to move that to the second lane. For that, You drag and drop on the second lane. And when you drag and drop on the second lane, then click analyze > gels>select next lane. And then you drag and drop from second to the third lane. same applies to the fourth. Hopes that helpful.

[@afreenhaque2024]

Hello, can you elaborate on how to quantify autoradiograph image?

[@simplicitas5113]

Funny how everyone has made blots of HSP90. Hilarious tbh.

[@jinghao6888]

every time I need to do the quantification I come back to this video. Thank you so much.

[@drkhushikhan7496]

Hi! I need help, how do I contact to you ? Please reply Thanks

[@s.p.productionpvtltdindiac6179]

Good one, keep it up

[@yousufali_]

Thank you, I will

[@ขวัญจิรารัตนะวัน]

Thank you very much

[@yousufali_]

You are welcome

[@premaagarwal3387]

It was helpful. Thank you.

[@VanessaOliveira-um7cb]

Thank you!

[@md.hasanuzzaman5607]

Thank you so much. Got what I needed.

[@yousufali_]

You are welcome.

[@premaagarwal3387]

After selection of the plot why the quantification is showing not only for the area under curve its also including near by places in my case. Any solutions?

[@yousufali_]

If I understand correctly, I would say you might have problem in plot selection. Watch carefully from 2:30. when you draw line it need to touch the both side of curve. LMK if you have more questions.

[@shayansara]

same here any solution?