Thank you for taking your time and giving an introduction to WGCNA :)
@KN-tx7sd2 жыл бұрын
Thank you, this is an excellent and clear presentation, mentioning the strengths and weaknesses of WGCNA. Can clarify whether WGCNA could be applied to metabolomics datasets. I'm seeing more and more papers using this approach. Do we need a minimum number of samples in such datasets and do we need to make any tweaks in the software to accommodate metabolites and lastly how could the interpretation be different from gene expression networks.
@eaturfeet6533 жыл бұрын
This clears up so much for me! I just recently started getting deep in to transcriptomic analysis for my PhD. Thank you for this!
@xudongwang-je6vo5 ай бұрын
YES, IT IS VERY VERY HELPFUL FOR ME, THANK YOU!!! SO MUCH!!!
@asiyazhao38203 жыл бұрын
Hi thanks for your video. just a quick question, do you adjust p-values for spearman correlation before you merge the correlation data? thank you very much
@OrlandoG3 жыл бұрын
Very good informatiion. Thank you for sharing this. 👍 Please don´t take this the wrong way, I know english isn´t your first language, but I find it difficult to understand your sentences at times because of your pronunciation. Thanks again and I look forward to more videos.
@LiquidBrain3 жыл бұрын
Thanks for your feedbacks. It brings me pure joy whenever I’ve finally understood something and able to share it around! I’m working to put up the subtitle there and glad that you found this video helpful :)
@grsbiosciences2 жыл бұрын
Madam I have gene expression data of control and drug treated, what I can find with WGCNA for my data
@LiquidBrain2 жыл бұрын
Hi you may try to explore more about its application from the official website: horvath.genetics.ucla.edu/html/CoexpressionNetwork/Rpackages/WGCNA/
@ruchichauhan54123 жыл бұрын
This was very helpful! Thanks a lot
@Unaimend2 жыл бұрын
To which paper are referring to at 8:40?
@LiquidBrain2 жыл бұрын
Hi Thomas, did you refer to the dynamic tree cut? I got it from the official WGCNA tutorial website (horvath.genetics.ucla.edu/html/CoexpressionNetwork/Rpackages/WGCNA/Tutorials/FemaleLiver-02-networkConstr-auto.pdf) -Lind
@MrRamaeri3 жыл бұрын
thank you
@ryankelly93023 жыл бұрын
Great video! Do you guys plan to do a tutorial on the WGCNA R package? Think it would go great with this video if you were able to use it with some RNA-seq data.
@LiquidBrain3 жыл бұрын
Ya, it's actually in the pipeline way before this video. But a full pipeline takes a lot of plan , film, edit and stuff~but hopefully we can get it done soon
@ryankelly93023 жыл бұрын
@@LiquidBrain Great to hear! I look forward to that video!!
@jianjiang82343 жыл бұрын
@@LiquidBrain I'm also looking forward to the video.
@WalyB013 жыл бұрын
Bad rehash of some else her video. Not cool using some else her slide!
@lindseyliquidbrain71393 жыл бұрын
We all are learning along the way. I did not rehash but try to summarize what I've learned after taking a long time to digest the formal knowledge sources (e.g. publication, tutorials, or webinar). You could just go ahead to read and study via the original sources as I cited above if you preferred.
@grsbiosciences2 жыл бұрын
Great explanation, how to know which module has genes related to a.particular phenotype. So how I relate my modules to traits in my case
@LiquidBrain2 жыл бұрын
Hi good question, you may refer to my tutorial videos. Part 1: kzbin.info/www/bejne/mqi6maBtbc9rj7c , Part 2: kzbin.info/www/bejne/a4fHoWyFopiad8k Happy learning! - Lind
@shinjayson6432 Жыл бұрын
This is wonderful. Thank you very much.
@mightyowl16682 жыл бұрын
Amazing video! Please keep making these!!
@angelac.16532 жыл бұрын
This video was great, thank you so much for making it! I've been trying to apply transcriptional gene modules to a project I'm working on. The approach I read about (Deep Extraction Independent Component Analysis) used ICA and artificial neural networks to identify the modules (the authors created the DEXICA package for R). WGCNA is an interesting comparison.
@LiquidBrain2 жыл бұрын
Glad it was helpful! The approach you mentioned looks new to me, will be great if it improves/overperforms the statistical algorithm by WGCNA
@jamesgalante9932 жыл бұрын
Thanks!
@aayushinotra57752 жыл бұрын
Hi can you please tell me how to install WGCNA R package. It would be great if you could help
@LiquidBrain2 жыл бұрын
Hi here's the script for installation: install.packages("BiocManager") BiocManager::install("WGCNA") you may refer to my tutorial videos too. Part 1: kzbin.info/www/bejne/mqi6maBtbc9rj7c , Part 2: kzbin.info/www/bejne/a4fHoWyFopiad8k Happy learning! - Lind