What is Strandedness in RNA-Seq data? | RNA-Seq Stranded Library Construction Methods

[@tolga1292]

Wonderful! Could you talk about STAR in your next video?

[@tolga1292]

In addition with Post-alignment-QC would be wonderful :)

[@lucasfleitasibarrola2322]

This was very helpull. Its been days since I was trying to understand the mechanism of this. Thank you!

[@sjwu571]

Hi loved your videos. Just want to point out, at 6:28, I think your labeling for Gene 1 is wrong. Please double check I'm not crazy. Thanks!

[@lucasfleitasibarrola2322]

Yes I also noticed that!

[@AndazeBayan1]

Just like your name, your work's khusbu is reaching to those who need it the most ❤ Keep doing this great work.

[@ThaoNguyen-sv2rg]

Great video Khushbu ! 6:14 Based on your explanation, for mRNA1, the antisense should be 3'5' and sense should be 5'3'. Do I misunderstand or is it the slide misrepresentation ? Thank you

[@gadhakleons]

I had the same question.. Were you able to clear that?

[@hridayhomechowdhury2142]

Thank you very much, I learned a lot from your tutorials.

[@MarwaTawfikBadawy]

Thanks a million, Khushbu! Kindly keep creating these videos and go ahead for the IGV video please!

[@reinhardgrausenburger4095]

Great Video! It would be great if you could also show us how to check strandness in IGV. Thanks a lot!

[@MF-fk4lp]

Would you be able to do a tutorial on pathway analysis for individual clusters in Seurat? GSEA, GO, KEGG, Reactome, ... any of these would be great.

[@chatomics]

I made a video on it recently kzbin.info/www/bejne/f3ymdYR7pdp9eqM

[@hinasultana2066]

Very nice presentation. I have a question. In stranded RNASeq, after the second strand cDNA is synthesized with dUTP, it is degraded. I am confused as to how the second strand information is generated later on , when that strand was degraded?

[@apostolosevgeniospavlos320]

Perfect presentation for someone who want to understand the differences between stranded and unstranded protocols. I have one question, how it is possible to understand the strandedness from GeneCounts option coming from STAR. In case of unstranded data it is clear that you ll choose the first column, but in case of stranded data how can you determine which column you choose between 2nd and 3rd?

[@kasmikaborah]

For biomarker identification which should I used like unstranded,stranded, reverse stranded

[@tushardhyani3931]

Thank you ma'am !!

[@divyaagrawal6740]

Thank you for the video Khushbu :)

[@hayabusa8319]

Please go ahead with IGV video...🤘

[@perthteluguchronicles]

Thank you very much, I learned a lot from your tutorials. Can you make a tutorial on aberrant splicing events using FRAZER please

[@aayushividhoy5943]

Hi your videos are soo good am currently preparing for a job in bioinformatics...if possible can you please guide us like what kind if questions will be asked and how we should prepare for an interview

[@naveennaveenkumar7127]

can u provide me your mail id I wanna talk with u mam regarding to r programming launage

[@Bioinformagician]

You can find my contact information linked in the description section of every video