What the H? Decoding the Bombay Phenotype

This presentation from December 2014 is a close look at the rarely seen but commonly discussed "Bombay Phenotype." We will discuss the basics of ABO and H antigen formation, then learn what makes the Bombay Phenotype unusual and different. This presentation accompanies the September 2014 podlet on the Acquired B Phenotype, also found on KZbin.

[@user-we8rb3rf4g]

man !!! you saved my life !!!! my midterm is tomorrow and I was literally crying till I find your video 😭 thank u so much you are better than my doctor I swear

[@meryemlahbara9669]

“We know Mom is mom but do we know Dad is DAD?” !!!! Funny

[@QlarityP]

So very helpful! Just the right level of detail and good graphics to support the audio information. Better than my actual professor's lectures and much more condensed. I appreciate the information-dense format. Like Blood Bank Concentrate!

[@bloodbankguy]

I'm glad it was helpful!

[@haifamansour24]

Thank you very very much! I'm having an exam on Thursday and this is really helpful.

[@PhillipH-san]

Wow, I saw my first Bombay last night and I've only been a tech for a little over a year. And I work at a relatively small hospital! Thank goodness she didn't need blood!

[@maitrayeedas493]

Thank you for the explanation! This was really easily understood as I had earlier thought the topic would be difficult!

[@techmedic7640]

Precised beautiful explaination.. Thank You!

[@kmaj9767]

Such a lovely presentation. THX

[@Animaniafreak]

Very useful video, thank you.

[@merrykendolor9623]

..hi sir..i am a medtech student..wow i am really happy and impressed that i found this video..somehow i get the point of this abo blood group system..i am hoping that you have any video regarding the abo discrepancies..i will surely visit your channel for a better knowledge about blood banking..i wanted also to feel that blood banking is easy to learn just like how you have mastered it sir..

[@Branables]

Hi! Great video! So naturally occurring Anti-H is a mixture of IgG and IgM? Is that why it's able to react at IS and 37 and AHG? Or is it just an IgM that is capable of reacting at 37C? Thanks for your help! Very interesting!

[@joseluisestrada614]

Hello great classs , very helpful do you have any information about ABO incompatibility in hematopoietic cells transplantation ?

[@crocketmeow]

The presence or absence of the fucose (H antigen) does not appear to affect the Rh protein. Is there still a way someone with an Rh+ phenotype could be an OH- (Bombay) phenotype/hh sese genotype? If so, how?

[@bloodbankguy]

Not sure exactly what you are asking me! The two are unrelated, yes, so it's absolutely possible for a Bombay person to be Rh+ (D+, to be more precise). Is that your question?

[@SandhyaRamachandran]

excellently done..thank you so much... Anti-A,B in O grp people at 10:57 ? i dint know that !! I saw that you call it weird... indeed... so is there an Antigen AB !...TIA.

[@afvelovic]

Hey how are you. I am a Med Tech and new to Blood bank, with previous experience in Hematology/Chemistry, Serology. I was so thankful to come across your videos and I have learned a lot in a short period of time, which I absolutely love. It honestly made my life easier. Just out of curiosity, do you have any video available on Elution procedure and if you do may you please share where to find it. Thank You so much.

[@bloodbankguy]

I don't actually have anything specifically on the elution procedure, BUT Sue Johnson, who was on my podcast recently (bbguy.org/028) does have one. Check her video at www.indianinitiative.org/lecture/evaluation-of-an-autoantibody/. Best wishes! -Joe

[@afvelovic]

Thank you very much for a prompt response. I will check out the above link .

[@SandhyaRamachandran]

SUPERBLY EXPLAINED

[@kalpanayadav2913]

Thanks a lot..this video is very helpful...

[@sabrinaburns3195]

So I have been told twice that I'm O+ however my son was born AB-. Should I get tested for bombay?

[@bloodbankguy]

+sabrina Burns There are many reasons why that could have happened. Bombay is one possibility, but the more likely explanation is found in your partner's blood type (potentially with an unusual inheritance pattern). It's unlikely that you would have gone through pregnancy without the discovery of the antibodies associated with the Bombay phenotype (the antibody screen that essentially all pregnant women get, at least in the U.S., would have detected the antibodies).

[@SandhyaRamachandran]

IN a blood bank, wouldn't a major cross match show agglutination and thus take care of the Bbay phenotype recipients who have been labelled blood grp O but were actally Bombay phenotype?

[@rafa-tm9hg]

Thanks a lot! Love the clarity of your explanations. Can bombay group donate to others?

[@bloodbankguy]

I think I answered this question for you in an email.

[@lisareed9296]

Your videos are helpful and much appreciated! When you speak of how Bombay presents as a type O; (20:38) I would think that their anti-H would show weak reaction to all types, AB and O. What am I missing? Thanks.

[@bloodbankguy]

+Lisa Reed Lisa, I'm slow to get to this; my apologies. They type as O because their RBCs react with neither anti-A nor anti-B, and their serum/plasma reacts against A1 and B test RBCs. You are absolutely correct that they would react against either AB or O RBCs, but we don't use those in most cases for routine pretransfusion testing in the US. So, Bombay folks usually don't have an ABO discrepancy. I hope that helps!

[@Alicesliced]

This may be a silly q but for Bombay people, do they not have ABO, Le or Se on their red cells?

[@bloodbankguy]

The point of Bombay is that the person lacks H on their RBCs as well as in their secretions. So, no H means no A or B on the RBCs or secretions, and no Lewis antigens in secretions (or adsorbed onto RBCs). There actually is no "Se" antigen; that's the allele that encodes the enzyme that puts H on type 1 chains in secretions.

[@KT1419]

It's possible that I have the Bombay phenotype, but I'm not sure how to go about testing that. Should I go to a local hematologist, or should I seek out one at a university? I asked a biology teacher friend of mine, but she'd never heard of (or didn't remember hearing of) the Bombay phenotype.

[@bloodbankguy]

+KT1419 Most hematologists (and essentially all blood bankers) would know what it means. Sorry it took me so long to respond.

[@KT1419]

That's okay. I'll try a hematologist around here. I'm mostly curious to see if I have the phenotype, particularly since it would affect transfusions. Thank you!

[@lyxenjagunap3714]

Thanks

[@barblilth3025]

My boyfriend donated blood many years ago. And was told he was oh + I have been trying to figure out what this really means. Can you please help me out

[@bloodbankguy]

There's a lot that could be said, Barbara, but I can't really do it remotely. My advice: Have him talk to the medical director of the blood center where he donated (or, if that isn't possible, find a local blood donor center and ask to speak to their medical director). Terminology counts in these cases, and if he was told something even a little bit incorrectly (or if he remembered a little bit off), the whole meaning could change. Best wishes! -Joe

[@SandhyaRamachandran]

so the baby 2 at 24:23 was Hh..?

[@johngaltspeaking213]

What the H?