Why highly aqueous mobile phases must be avoided when using C8 or C18 Column?

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[@maheshwaghmare8346]

VERY VERY NICE EXPLNATION

[@sreekanthpillodi9950]

Jai sir, reverse pahse techinc peptide molecule after if loandIng 100 gm compound passed organic buffer total volume assay 40 gm reamaing 60 sticky solid observed when landed 300 DAC please respond sir

[@basilwilson3929]

How the ACN and methanol become non-polar? You mentioned they are non-polar

[@RashidAnsari-dk9mk]

I think by mistake