Why Individual Cells Can Be Hard to See Under the Microscope

[@aliprovidence9321]

Excellent short video and very informative Thank you, Oliver!

[@ihspan6892]

I truly love your videos!

[@Microbehunter]

Glad you like them!

[@kennethpon9498]

So fascinating. In the late 60s as a high-schooler I spent hours looking at protozoa and simple multicellular organisms. Thanks! Brought back memories.

[@Microbehunter]

Glad you enjoyed it!

[@grannoid]

Great video, really interesting!

[@Microbehunter]

Thank you!

[@JohnMichaelson]

The image quality you get in these videos is every bit on par with any other channel on KZbin, Oliver, it's amazing! The clip at the end is the sharpest I have ever seen the internal details of the rotifer. Every time I watch I feel I must chain my wallet away lest I overindulge in another expensive hobby.

[@smartchoices2000]

Thank you for sharing your knowledge! It was very informative to see in real-time the differences in Eukaryotic and Prokaryotic cells.

[@Microbehunter]

Glad it was helpful!

[@bradnelson3595]

A very nice presentation. And I definitely learned something new. Nice images as well.

[@Microbehunter]

Glad to hear it! Thank you!

[@mikevanderman2727]

Too interesting if you ask me, both visual and informative, love it!

[@Microbehunter]

Awesome, thank you!

[@Absolotle]

Great video, thanks for the explanation.

[@Microbehunter]

Glad you enjoyed it!

[@JB-ol4vz]

Lovely, That was one off the most interesting explanations you've done in a long time. More off this pleas5. Thank you very much Sir. Cheers from Sweden

[@Microbehunter]

Thank you for this feedback. This is important. I do sometimes have trouble finding what people are interested in. I was already worried, that the discussion of refractive index etc. is already too specific and technical.

[@websurfer5772]

@@Microbehunter I find those topics interesting.

[@philsillett7262]

Excellent thank you. Explained it so even I could understand it. Like the water in the glass analogy to explain refractive index.

[@Microbehunter]

Glad it was helpful!

[@nicklong9985]

What magnification is that?

[@Microbehunter]

Different ones, maximum objective was 60x, so these organisms can be seen with conventional compound microscopes.

[@rahmahussien2831]

Have you tried staining onion cells with potassium permanganate? I used it with ethanol and water instead of using ink, it stained nuclei but also other structures that I couldn’t identify

[@Tybold63]

Learning something everyday, very good explanations too.

[@Microbehunter]

Glad to hear that!

[@amethyst2448]

Wonderful!

[@MarieChardome]

waaaaw, MH, this is very interesting news to me, the line that is not a line but a difference in refr index! 1. phase contrast seems the best rendition of this fact // 2. so that's why we can see bacteria better with Ph Co // 3. then maybe 'texture' is a way to find groups of cells with a similar job 3. if P. bursaria always has algae, it becomes de facto a multicellular animal (like mitochondrion became part of cells). fascinating. thank you

[@MarieChardome]

also, the visibility of the algae inside P. bursaria is not only color, but being a plant, it will have a visible wall? oooh, you got my head spinning now

[@Microbehunter]

To be precise, the dark line around the cells is due to the diffraction of light (due to a difference in refractive index). When you look at an air bubble under the microscope, then you will also see that it has a very dark (and thick) line. This is also due to the difference in refractive index between water and air.

[@MarieChardome]

@@Microbehunter amazing

[@raulpulgarsepulveda6296]

It could be interesting to label the nuclei with DAPI o another molecule.

[@lotharmayring6063]

why you talk about refractive index, color and staining since everybody has phase-contrast and DIC

[@sannee3715]

your comments are annoying

[@Microbehunter]

The same concepts also apply to brightfield microscopy.

[@lotharmayring6063]

@@Microbehunter if you dont see details wich have the same refractiv index then use PH or DIC even darkfield helps

[@lotharmayring6063]

@@sannee3715 das ist mir sowas von egal

[@lotharmayring6063]

@@Microbehunter ich verstehe ihen Komentar garnicht...Die Methoden PH und DIC sind auch nur Varianten einer Hellfeldmikroskopie mit denen man tierische Zellmembranen durchaus darstellen kann

[@kapa1611]

👍