WHY RT SHIFT | HPLC TROUBLESHOOTING | HINDI

[@mahatabali2555]

Excellent, sir.... Clear concept

[@voiceofkayani5419]

Keep watching and sharing

[@nadeemiqbal4742]

Thank you sir.

[@AnujKumar-if4ep]

Buffer ratio increase krne pr kya effect hoga

[@voiceofkayani5419]

It may change the mobile phase pH which effacts the peaks shape and retention time

[@197_sanketkumarthakar6]

Sir aap hplc ki column konsi use hoti kese karete he

[@voiceofkayani5419]

Meack column

[@197_sanketkumarthakar6]

@@voiceofkayani5419 residue solvant , chiral, assay ae sab Kay hota he

[@voiceofkayani5419]

@@197_sanketkumarthakar6 dear, Different test Hain in raw material

[@karuneshwaripatel8796]

Sir please share stability guide line

[@voiceofkayani5419]

Ok

[@vasudevsharma-ey6ni]

If methanol quantity is increased then how will it impact on rt ....?

[@voiceofkayani5419]

RT will be shift. Suppose was 15 minutes It may 13 minutes if methanol quantity increased

[@vasudevsharma-ey6ni]

Rt me Kitna shift acceptable hai ?

[@akshaypanaskar5772]

10% of actual rt

[@voiceofkayani5419]

Aprox 10 %., At 1°C temperature variation aprox 2%