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Hello Dear, I have referred to the regular rDNA technology books .

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Hi there Thanks for your interest You can reach me via [email protected]

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Welcome sir, Wonderful to know that we belong to same community. I shall try to create content.

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@@Biology60___ hi, it won't be very effective in himdi

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Hi, Thanks for the nice comment You choose the Expression Vector with various tags (For example GST tag) to increase the solubility and stability of your protein. I suggest expression vector with GST tag may serve your purpose.

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Any gene can be expressed in baculovirus expression irrespective of the origin of gene. Try to subscribe channel to get regular updates.

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Mutation in Cos sites of Lamda DNA can not undergo replication but can synthesize all phage proteins. Such proteins are purified and mixed with recombinant lamda molecule and then phage particles are formed in vitro. Mutations in Cos sites lambda DNA phage strain is sufficient to pack the recombinant lamda DNA.

One of the variant of Mammalian TDT have 3'to 5' exonuclease activity

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Hi, During the retroviral infection, by using Reverse transcriptase RNA is converted into double standard DNA and that is integrated into host genome. Eukaryotic mRNA will be used as template to synthesize cDNA by employing Reverse transcriptase.

@@Dr.DNA-Primersir reverse transcriptase requires a primer, which in the case of retroviruses, is a tRNA molecule bound at a specific site (the primer-binding site) close to the 5' terminus of the viral RNA

Yes

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No, double standard M13 fragment will be inserted. M13 genome converted into double standard that is called replicative form. Those replicative forms can be isolated.

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Thanks a lot sir 🙏 I was really confused about this

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Chemical Synthesis only

During in vivo replication primers are synthesized by primases only

For pcr and RT-Pcr chemically synthesized primers are used

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Generally Reverse transcriptase do not have 3' to 5' exonuclease activity (Proof reading activity) so no correction of wrong nucleotides added. Thanks

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