Hi, great video. I am attempting to do this for SDS-PAGE gels rather than WB ones. I am trying to compare Rubisco content and purity from various treatments. However, unlike WB where you probe for a target protein (e.g Rubisco), and get one nice band, my SDS-PAGE gel has many bands other than my POI. In this case, where would I subtract the blank from as there are no 'empty' spaces in the lanes? I thought of taking three measurements at the top of the gel (not in the lanes) and taking an average of this as the background. Do you have any suggestions? Also, I am not sure about the case where there are multiple bands per lane and the fact that I want to find out relative purity and compare the relative quantity between treatments (using a standard curve generated from Rubisco quantitation standard) if I should use the integrated density method or the NIH (AUC) method? Please give me any tips and suggestions. Thanks!

Original Paper Electrophoretic Transfer of Proteins from Polyacrylamide Gels to Nitrocellulose Sheets: Procedure and Some Applications: www.pnas.org/content/pnas/76/9/4350.full.pdf

References to deepen your knowledge : Books "Principles of Instrumental Analysis" by Douglas A. Skoog, F. James Holler, Stanley R. Crouch This book provides comprehensive coverage of analytical techniques, including the use of internal standards in various instrumental methods. ISBN: 978-0495012016 "Quantitative Chemical Analysis" by Daniel C. Harris This text covers the principles and practices of quantitative analysis, including the application of internal standards in different analytical procedures. ISBN: 978-1464135385 "Handbook of Analytical Techniques" edited by Helmut Günzler and Alex Williams This handbook includes detailed discussions on various analytical techniques and the use of internal standards to improve data quality. ISBN: 978-3527296539 Articles and Papers "Use of Internal Standards in Quantitative Analytical Chemistry" by Alan G. Marshall and Bruce S. Meyer Journal of Chemical Education, 1977, Vol. 54, No. 10, pp. 640-643. This article provides an overview of the principles and applications of internal standards in quantitative analysis. DOI: 10.1021/ed054p640 "The Role of Internal Standards in Quantitative Analysis by Mass Spectrometry" by Chris B. Stack, David Muddiman Analytical Chemistry, 2011, Vol. 83, No. 12, pp. 4649-4660. Discusses the use of internal standards in mass spectrometry to enhance quantitative accuracy. DOI: 10.1021/ac200160w "Application of Internal Standards in Chromatographic Analysis" by James W. Taylor Journal of Chromatographic Science, 2002, Vol. 40, No. 6, pp. 292-299. Explores the use of internal standards in chromatographic methods to improve quantification. DOI: 10.1093/chromsci/40.6.292 Online Resources National Institute of Standards and Technology (NIST) - Analytical Chemistry Division The NIST website provides guidelines and resources related to analytical methods, including the use of internal standards. NIST Analytical Chemistry Division International Union of Pure and Applied Chemistry (IUPAC) - Compendium of Chemical Terminology (Gold Book) The IUPAC Gold Book offers definitions and explanations of terms used in analytical chemistry, including internal standards. IUPAC Gold Book Practical Guides "Quantitative Analysis Using Internal Standards in HPLC" by Jürgen Hochstrasser A practical guide on the application of internal standards in high-performance liquid chromatography (HPLC). Available online as a technical note from various chromatography companies.

Hi! What temperature do you usually spin the sample at? Room? 4ºC? Thank you so much!

The teaching methodology is not good...

bullshit, not helpfull at all

I did DNA extraction by method of Maxi prep for the first time. I didn’t even understand the others steps. I’m willing to learn more methods and other steps.

Greetings from America. I love your teaching approach Adwoa. Your channel is one of my favorites! Can you create a video similar to this showing how internal standards are used for a calibration curve. From what I understand ratios are key for the internal standard approach. Any information on this would be much appreciated!

Hello Doctor! I was diagnosed with HTLV-1, I have hyperthyroidism, osteoarthritis in my spine and osteoarthritis in my knees (I had surgery on my spine and a knee replacement). Do these bone problems and hyperthyroidism come from HTLV-1? I am 64 years old and female! A hug from Brazil!

I want to ask a question. My English skill is not enough to understand . It could be silly question sorry . I am trying to learn statistics . I would love to do statistics of my researches my own . I want to do a research about "Mortality and complications after percutaneous endoscopic gastrostomy)PEG): a retrospective one-centered study" My universe is 908 PEG . After excluding criterias I dont know how much will stay but probably around 600 cases I want them all. How can I calculate for that retrospective research sample size. 1-After PEG minör complication rate : %18-38 major complcation %2-4 2-After PEG mortality rate in 30 days : %3-23 I want to do multiple regression analyses . I have 2 dependent variables "mortality after peg " and "complications after peg" I have 31 independent variables. I do calculate at GPower . F test- Linear multiple regression : fixed model, r2 deviation from zero: A priori Effect size 0.15 , a 0.05 B: 0.95 number of predictors 31 Sample size 264. ***My question is this how can ı find effect size? Do you suggest me any other advice for my research sample size calculate ? Thanks for helping and sorry for baby english and long question.

Isolation of CTCs are also another popular use for SepMate/Leucosep tubes.

Download g*power here: www.psychologie.hhu.de/arbeitsgruppen/allgemeine-psychologie-und-arbeitspsychologie/gpower

Additional Essential Cell Culture Calculations (making up media and determining amount of drug treatment): kzbin.info/www/bejne/ZmXcZXeiZdqHd5o

Can a man be a carrier and not even know?

Can this virus 🦠 cause CML?

Excellent ❤

Thanks

Reference 2): Sequencing artifacts derived from a library preparation method using enzymatic fragmentation: journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0227427&type=printable

Reference: DNA Library Preparation Methods for Next-generation Sequencing: Tone down the bias www.sciencedirect.com/science/article/abs/pii/S0014482714000160

Thanks!

here we join the base with a line manualy which is error prone for the area calculation

References: 1) High rhesus (Rh(D)) negative frequency and ethnic-group based ABO blood group distribution in Ethiopia: www.ncbi.nlm.nih.gov/pmc/articles/PMC5530478/#:~:text=In%20Africa%20and%20Asia%20the,negative%20phenotype%20is%20even%20rarer. 2) Chapter 2: Blood group antigens are surface markers on the red blood cell membrane by Dean L. Bethesda (MD): National Center for Biotechnology Information (US); 2005. www.ncbi.nlm.nih.gov/books/NBK2264/#:~:text=Blood%20group%20antigens%20are%20either,the%20transfer%20of%20sugar%20units. 3) Blood groups (Better health channel VIC health) www.betterhealth.vic.gov.au/health/conditionsandtreatments/blood-groups# 4) Blood Groups and Compatibilities www.rch.org.au/bloodtrans/about_blood_products/blood_groups_and_compatibilities/

Hi Maame Adwoa, please i have a malaria vaccine to analyse the protein content. I have the Bradford Protein kit from thermofisher and the Albumin standards for the curve. can you help me with how to go about it?

Very nice presentation

Hi, thanks for the video. Please, I have a question: I will like to ask a question about cell treatment experiment with a test drug for 72 h. In such an experiment, do I have to add my drug once and incubate for 72h then check the cell viability or I have to remove the drug solution and replace with the same drug and concentration after each 24h to avoid wrong readings due to degraded drug in wells or evaporation? Please any other person reading the comments can also reply me if you have the correct answer to my question. Thank you

hi thank you for your explaination. I have a question, if I measure dose dependency, in specific, I measure basal value of each well for 10 mins, and then add agonist and measure for 30 mins more. Then its supposed to have many points, then how should i choose value to make Z' factor?

I am a complete novice with ELISA but i need to run some blood tests that the UK does not offer, im going to buy all the equipment to conduct the following tests: MSH TGFB1 VEGF MMP9 C3A C4A Can you recommend a good book for the protocols and what elisa tests would be appicable for these tests (dircect/indierct etc.....) The top one is melanocyte stimulating hormone and i need to see a quantity not just sample presence in all of them Thank you, great videos

How do you adjust the gain?

Thank you so much for this practical lecture for university students like me who cannot have the condition and biotechnological facilities to practice. I wish you all the best in 2024, Madam!

does the volume of the samples have to be constant

Really helpful thank you !

Why do we need to be able to do such calculations? In order to maximize all available storage space most solutions are stored in a concentrated form (known as stock). These solutions are then diluted to the required strength as and when required. This also means the same solution substance may be used for a different range of needed concentrations.

Thanks for the information. Love it

7:36

Great video 👍

keep up the goodwork. I hope someone there's multiple of someone like you in this world. You keep the world turning

Thank you! ❤️

What is a good H-index? Hirsch suggests that after 20 years of research, an h-index of 20 is good! It means that you have 20 publications with at least 20 citations. 40 is outstanding, and 60 is absolutely exceptional.

I analyzed your channel and I was impressed to see your video quality. your video thumbnail is very eye-catchy. However, you are not getting proper views and subscribers. Below we found some problem for your channel - Your video title is not SEO friendly Don't have proper tags and keywords Don't have meta tags for video ranking Descriptions are not optimized Please fix this problems to get more views and subscribers

I struggled to listen to it due to the music which is a shame as sounds like it may have been useful 😢

Other terms used to describe water quality in the lab includes: MQ/MilliQ water for type 1 water; Elix water for type 2 water and RO or demineralised water for type 3. Note that distilled water is water that is clarified through heating the water until it enters vapour form, and then cooling the water back to liquid form. Most labs do not use this method of clarification, preferring to use reverse osmosis, due to its greater efficiency.

Of course it is obvious that we do not appreciate qualities and the cost that it rather saves.

you saved my life man

Thank you!!

Fun fact: a typical adult has a blood volume of approximately 5 liters. Females and males have approximately the same blood percentage by weight.

Hi Adwoa Could you explain the interpreting Elisa result a bit more Do you have one-2-one tutorial?

Bold woman, magnificent

Note: some labs avoid using antibiotics in their Media as they don't want to risk masking low level infection or worse, facilitate the emergence of antibiotic resistant strains. Get more comprehensive knowlege via this article from pubmed: www.ncbi.nlm.nih.gov/pmc/articles/PMC7149418/pdf/main.pdf

PBMC will be used to assess viral infections, thanks.