🔴 Calculating Amount of Drug Needed for Cell Culture: Practical Guide

[@faisalrehman4306]

Thanks, howevr, the formula used here is :C1V1= C2V2, Where C1 =50mg/ml, V1=to find, C2=0.4mg/ml, V2=200. Cheers:)

[@adwoabiotech]

Note: some labs avoid using antibiotics in their Media as they don't want to risk masking low level infection or worse, facilitate the emergence of antibiotic resistant strains. Get more comprehensive knowlege via this article from pubmed: www.ncbi.nlm.nih.gov/pmc/articles/PMC7149418/pdf/main.pdf

[@adwoabiotech]

Why do we need to be able to do such calculations? In order to maximize all available storage space most solutions are stored in a concentrated form (known as stock). These solutions are then diluted to the required strength as and when required. This also means the same solution substance may be used for a different range of needed concentrations.