Thank you for this fun experience 😂❤

These people playing crazy rock music in the background is the worst thing for a science video

Are you also provide trainings?

Cringiest video out there. “Ughhhh”

I'm building my own genetics lab at home 🏡

What is the point of a lab coat if you got your hood on it? Also, your lab coat sleeves are too short, so your bare skin comes out, this is why cuffed lab coats are much better, and it is easy to put the cuff in your glove. That shelf obstructs the airflow at the back of the BSC, it shouldn't be there, there is a lot of stuff that is not required for current work and has to be cleaned up in case of spillage. The handling of the bottles is also sacrilegious in both cases, you should avoid putting away the caps, you should put the pipette back in the packaging to not scatter stuff what is left in it around. I use 0.1 of a culture volume of trypsin, so for T25 it would be 0,5 ml. Glass pipettes can be easily replaces with cheap pump nozzle that fit standard one-use pipette tips. 500 g is a recommended force for precipitating the cells. From earlier I assumed you use a medium containing serum, so unless you switch to serum free media that centrifugation step is completely unnecessary. FBS contains a very strong trypsin inhibitor. You also lift the bottles when not necessary, risking dropping them and spills, this can be comfortably done without all that lifting and putting away caps. You also touch your glasses when removing cells from the BSC - this is unacceptable. You are also not supposed to open anything that may contain living cells outside the BSC.

This video is amazing! Great job!!

Oh my goodness, I can't believe after suffering from PCOS for good 11 years I can still recovered back my health and the natural herbal supplements I ordered it from DR AKHO HERBAL on KZbin, I'm totally free now keep saving lives ...

nice video! pls make more videos like this. Thanks

Nice video

This is so nerdy. I LOVE IT. L0l. - Tissue Culture Analyst

When she was aliquoting the master mix to the last well there was some reagent stuck in the tip and she just threw it away . And when she banged the plate on the table, this will easily cause aerosol contamination . Bad video .

3:28

Lmaoo I wish my lab made videos like this

this goes to my favs Gracies profe de genetica per posar aquest video als powers <3

I hope you have an audit soon. Too many things in the PSM ! Absolutely wrong

Thank you Dr. Clean.❤️❤️

Very nice, and helpful. Very helpful comments that complete information. Toghether forms corect tehniques. Good.

Hi, do you know if this would work with proteins?

0:21 05:42 02:19

Not enough spraying of ethanol. Not aseptic enough

JAJAJAJAJAJAJAAJAJAJJAJAJAJAJJAJAJAJAJAJ

😂😂😂👊🏻

Thanks for sharing sir

You are using negative photoresist SU 8? Why don't you turned onn uv light for curing?

Plz make serious videos not include this distraction by Dr contamination

I saw dr clean groping dr contamination under her hood without protection. But thats none of my business.

Why there is are some classical music there, hahah I can’t focus on the lecture, cuz the music is too beautiful !

Good tutorial!

I wonder why she added the master mix into each well with inconsistent volume??

Dr. Clean is so bad....

The way dr contamination is included in this video is distracting and annoying

Der kha wallah...nar pashteen bahdar pshteen... Manzoor pashteen manzoor pashteen

Who the hell is the guy with fvckin nose and wig? Distraction

Plz no music its just irritating

Amateur hour in this lab.

Thank you

The weak link in aseptic technique is the glass pipette container. They are crammed in the metal box necessitating grabbing one while touching the others, then pulling the one out the possibility of tip contamination results. I use my finger tip to carefully push up just the pipette I will use and avoid touching the others.

Notice how at 4:30 the lid for the PBS wasn't inverted before being laid on the counter. Bad technique which can lead to contamination.

I didn't understand where the hell that pallet gone? 🤔

I love this video!!! This is so helpful and super funny. Thank you!!! 😊❤️❤️

dam mad, keeping hand out to put used pipette every time, kept unwanted material in bio safety cabinet, no mask, head cover.

NO FOOD OR DRINK IN LAB

They just give doctorates to any ole schmuck nowadays.

Always put the centrifuge cap upside down

OMG, I like it!!

wanted to use this in the class but for its music ......

minute 8:44 supernatant aspirated minute 9:46 magically media appears in the test tube. Sorry I missed the step in between. After aspirating the supernatant do we add fresh media? If so how much ml? Thanks.

For those who are not familiar with centrifugation, you may have to ignore the 1,000 rpm recommendation in this video. Depending on the rotor size, that means you may be applying less or more gravitational force than you want to. Potentially, using 1,000 rpm for your centrifuge setup will result in not collecting all or damaging cells in the process. It is more helpful to give parameters for centrifugation in # x g (meaning some number times gravity). x g is always equal no matter what centrifuge you use, whereas rpm is dependent on rotor size and is associated with a specific x g for that rotor size/centrifuge model.

Can i use this same method to attach an acrylic substance to glass?