Setting up PCR Reactions

How to set up a PCR reaction on the Roche LightCycler 480

[@wackles123]

I wonder why she added the master mix into each well with inconsistent volume??

[@matthewbarnett3540]

why are all of your tip boxes open? especially when doing PCR. Contamination is imminent

[@annaleevazquez418]

0:21 05:42 02:19

[@huiyingtanyayap]

When she was aliquoting the master mix to the last well there was some reagent stuck in the tip and she just threw it away . And when she banged the plate on the table, this will easily cause aerosol contamination . Bad video .