Wow, thank you!

This can vary greatly. As a default, we'd always recommend checking with your instrument manufacturer's guidelines. These are generally around every six months. How much you are injecting, how much is your split, how dirty are your samples can all impact this. Cleaning split vent lines is generally reserved for a situation where you feel it is needed, such as seeing some ghost peaks or other evidence of contamination. In an extreme case, when you notice incorrect flow rates and may suspect some clogging.

Hello, I'm sorry, this is not a product that we offer. Thank you for asking!

Generally yes, but it doesn't need to be.

What are you injecting and how much? You may wish to troubleshoot by trying to inject less or a different solvent. You can also do an no injection instrument blank to see if it is a timing/flow issue rather than the injection itself. You can find instructions on how to do that here kzbin.info/www/bejne/qqHIk4Oeq7Z4iLssi=DNIgtobWEzHsxpe3

For any specific column, we would always recommend referencing the original manufacturer's instructions. Here is some information that may be helpful www.agilent.com/cs/library/datasheets/public/820629-008D.pdf For general information on LC column regeneration, we have a nice blog post www.restek.com/chromablography/resurrecting-an-old-reverse-phase-lc-column--should-i-could-i-how-would-i

In the past, the serrated edge was recommended for use for cutting some smaller ID metal columns. Generally, even those are now recommended to be cut with the smooth edge.

@@RestekCorp Thank you for the information.

Could you please make a complete video for working Software and analysis details step by stem

I also encounter the same issue.

Compounds like carboxylic acids, can be very difficult to get good peak shapes. Depending on the column phase, there may be columns specifically designed to work with acids that can help. Stabilwax-DA columns are an example. Derivitization is also used for some compounds to improve peak shapes - fatty acids are often methylated before being analyzed for this reason. Some analysts also do priming injections - injecting high concentration standards to help occupy active sites before doing their analytical injections. There are many options, but they can all have their pros and cons.

The pressure controls of the GC system are designed to handle these pressure changes. So while there is still flow going through the column (and out the split vent, septum purge, etc.) pulsed injection allows users to temporarily elevate the pressure in the injection port.

@@RestekCorp Great! Thank you for this.

You're welcome!

That will depend on your instrument. Many can use standard compression fitting caps, but you should check your instrument manual or instrument manufacturer to be sure.

Tailing happens when the analyte of interest retains in the column. How the oven is programmed, gas flow rate, or the column used could impact your acid. You might need to look into different columns since you are dealing with aromatic acids, like Chromaton N-AW-HMDS with 5% Apiezon L column.