🙏🙏🏻😊

She didn’t change her gloves when she handled the gel and then touched the microwave .. yikes contamination factor

What is the purpose of the aspiration tube?

Sorry but I can’t take someone who clearly has no clue about aseptic techniques seriously when they’re trying to show others how to be aseptic in the lab/cleanroom lmao

Should i use nutrient broth instead of LB for Bacterial DNA Isolation @Addgene

V good

nice video i learn so much

Ho

Hi

Hi

Hi

Hi

Hi

The "OTHER" Methods for introducing plasmids to dns being the COVID vaccine.

Should the 50% glycerol be sterilised/autoclaved?

Thank you so much 🥰

good ass video

Very helpful video. THANK YOU

The music becomes so loud I can not hear what is being said.

شرح بيوتكنولوجي باللغة العربية: youtube.com/@manaljarrar?feature=shared

Great👍

Thanks for this video. It has been helping me a lot.

So useful and informative, Thank you so much..

How long can a lab coat be worn before it is to be discarded?

Superb,please take up the topic of micro manipulation

nerds

perfect video!

Hi I need to work with you i too needed to video like this in my own language can you let me know where i can contact you

Super

Thank you 😊💕 so much 🥰🥰

❤❤ i really enjoyed and understand it 😊😊😊

Did anyone else watch, thinking this was related to sign language…? 😂

👍🏻👍🏻👍🏻

No doubt these plasmids can be used for good or bad reasons

Ohhh thanks alot...needed in med school❤

Thanks for this. No one is an island of knowledge

love her gloves

Working in Addgene is my dream.

Is it safe to touch the gel?

This is such a clear video and so well explained. Very easy to follow, thank you

Oh your happiness at the end😁🤗

my mans streaked it so wrong. you cant scratch the agar like that

👏👏

This is extremely helpful for my research. I appreciate how clear your explanation is!

I'm falling in love with you all guyzzz , lots of appreciation and love ❤

I think that you must add 8 ul from carb not 80 ul

Nice video overall, just a few notes: 100 degree Boiling for 15 minutes is ill advised and will lead to protein hydrolysis for many proteins. 95C for 5-10 minutes, or 70C for 10-20 minutes is more than plenty for 99% of western blotting applications. 150-200V is fine with Invitrogen's Bis-Tris Gels, especially if you use cold Running Buffer to start. For Invitrogen Dry Transfer pre-assembled sandwich, they SPECIFICALLY advise to not activate the PVDF membrane as it is already pre-activated. Washing with Blocking Buffer is only necessary when using blocking buffers that are resuspended just prior to using. This is to get rid of any particulates. With commercial blocking buffers, or stock solutions of blocking buffers that are already in solution, you can proceed immediately to adding the primary antibody after the blocking step. Fluorescent labeled primary antibodies are quite nice as well. lastly and most importantly: adding the HRP substrate onto the membrane while shaking it leads to much more consistent detection rather than placing the membrane on top of a puddle of the reagent...This won't disperse as evenly and there's a decent chance it won't cover the membrane evenly. The sheet protector is a nice thing to have as it'll keep your blot from drying out during long exposures.

very useful and detailed. it helps me a lot.

Is plasmid taken from blood fractions?