Hi, I'd like to know where you got the information that the known distance is 0.62 to make the scale bar, please?

thank you so much

你好，感谢UP的分享。我有一个疑问，在圈选细胞范围时，是不需要很精准的圈住细胞吗？Mean=IntDen/Area圈的范围越大会导致结果偏低吗？

pleasse send for me the file, where can i find it!!???

想问一下能用imagej计算荧光衰减速率吗？该怎么做

Thanks for your vdo

I have pics for MCF 7 cells in vitro stained by Phalloidin 488, how I can analyze them please? 😢

Very helpful, could you tell me how to mention t test in the graph?

thank you so much.

I added the scale bar to the image and now when I save it, it doesn't show the bar, I did several times but didn't, can anybody tell me what to do? I'm new on imageJ I don't know much about

nice video, I am assuming that you have to get the distance in pixels and known distance from your microscope that you used to take the pictures right?

This is really helpful video to know immunofluorescence quantification!

you saved my life friend

how to measure DAB intensity ?

Is this method correct tho? The programm calculates the mean intensity of the area you circle in. Imagine you make a big circle around your cell, the mean will be lower because most of the area is just black. If you now substract the mean intensity of the same circle in a empty area, your intensity will decrease even more And When you do a smaller circle just slightly bigger than the cell, the mean will be higher and if you substract the backround of this circle it will not substract that much because the backround is not even big. So the mean intensity will only be determined by how big you set the circle you want to measure and that is not comparable at all

Thank you

Veeery helpful thank you

Please complete the statistics.

Awesome video!! This really helped

Help me pls. How can I measure a timelap? Like 60 pictures in one file and i want to measure the process of changing intensity. I can only measure one at a time but cant let it measure 60 times each for a picture automatically