flourescence intensity measurement

[@tayloryoung2959]

Awesome video!! This really helped

[@kdonsky6]

nice video, I am assuming that you have to get the distance in pixels and known distance from your microscope that you used to take the pictures right?

[@user-sk7cr5qx3w]

This is really helpful video to know immunofluorescence quantification!

[@najyajabeenpoolakkalody3862]

thank you so much.

[@rabiazeb9051]

I added the scale bar to the image and now when I save it, it doesn't show the bar, I did several times but didn't, can anybody tell me what to do? I'm new on imageJ I don't know much about

[@songohan393]

Veeery helpful thank you

[@user-ql1nu5os5s]

you saved my life friend

[@wisamalton162]

I have pics for MCF 7 cells in vitro stained by Phalloidin 488, how I can analyze them please? 😢

[@tsmylovelybay]

Thanks for your vdo

[@gautamnisha7193]

Please complete the statistics.

[@doughnut469]

Help me pls. How can I measure a timelap? Like 60 pictures in one file and i want to measure the process of changing intensity. I can only measure one at a time but cant let it measure 60 times each for a picture automatically

[@user-yu1hb8pp5k]

using macro in image J

[@soumeyabenamar9790]

pleasse send for me the file, where can i find it!!???

[@user-yj4qp6rk5n]

你好，感谢UP的分享。我有一个疑问，在圈选细胞范围时，是不需要很精准的圈住细胞吗？Mean=IntDen/Area圈的范围越大会导致结果偏低吗？

[@user-yu1hb8pp5k]

尽可能地标出边界，染个phalloidin之类的或者照个明场，就知道细胞边界了，或者可以设置threshold这样可以得到total intensity。 再不行跑个western啥的，通过其他手段来证明你所要quantify的蛋白的量的变化

[@muneezalodhi1877]

how to measure DAB intensity ?

[@songohan393]

Is this method correct tho? The programm calculates the mean intensity of the area you circle in. Imagine you make a big circle around your cell, the mean will be lower because most of the area is just black. If you now substract the mean intensity of the same circle in a empty area, your intensity will decrease even more And When you do a smaller circle just slightly bigger than the cell, the mean will be higher and if you substract the backround of this circle it will not substract that much because the backround is not even big. So the mean intensity will only be determined by how big you set the circle you want to measure and that is not comparable at all

[@user-yu1hb8pp5k]

you can use the threshold and then do analysis particle to choose the positive area only

[@qichengdu7400]

想问一下能用imagej计算荧光衰减速率吗？该怎么做

[@user-yu1hb8pp5k]

这个你得另请高明了