Julian Liber

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@theresazelazny7445 12 күн бұрын

How does the high ph of the ammonia affect the integrity of the protien fiber. Protien fibers do better with low ph. Did you use a mordant to lock the color into the fiber? If so what kind? Did you let the wool cool to the same temperature as the rinse water so it wouldn't felt? Is the color persistent or fugitive in the sun over time? does it wash well? Finally it's best not to wring out wool skeins, instead roll the skien in a dry towel. You made a very nteresting video.

@0yodelingpickle526 18 күн бұрын

You should NOT be ringing your yarn like that - spin it in a lettuce spinner. Ringing it stretches it which is not good, especially for wet wool.

@erikawerner 2 ай бұрын

Thanks for the interesting video. Are you from the San Diego area? Your Name sounds familiar .

@Gwenny89 2 ай бұрын

Will it stay purple after long years of sun exposure?

@julianliber4633 2 ай бұрын

@@Gwenny89 it will fade gradually with sun exposure. Unfortunately most naturally-derived pigments are less colorfast than synthetic ones.

@Bearfoot-e3e 2 ай бұрын

I always knew this lichen was special. Although, ammonia is not something I like to mess with. Pokeberries make purple too.

@tawseefalaff9976 2 ай бұрын

Is the isopropanol ice cold or room temparatyre

@julianliber4633 2 ай бұрын

@@tawseefalaff9976 I typically chill it for this protocol, but if it's at room temperature then you can likely put it in the freezer for 1 or 2 minutes longer.

@tawseefalaff9976 2 ай бұрын

@@julianliber4633 Thank you so much

@modo5091 2 ай бұрын

Hello thanks for your video, i am now fermenting my own! will this colour fade in sunlight?

@elizabethannegrey6285 3 ай бұрын

Absolutely mesmerised. Thank you for this video, and your dedication to keeping an ancient craft alive.

@cheezyasf 3 ай бұрын

Does it need to be heated ?

@julianliber4633 3 ай бұрын

Yes, I believe so. It is possible that the dyeing will work offer a long time at low temperature, but I haven't tested that.

@LK1zt 3 ай бұрын

I added lime of milk. Does this affect the target product?

@julianliber4633 3 ай бұрын

Is that in addition to ammonia or instead of it? Raising the pH generally makes the color more purple but otherwise shouldn't effect the dyeing.

@LK1zt 3 ай бұрын

@@julianliber4633 I added it during fermentation

@julianliber4633 3 ай бұрын

@@LK1zt as long as there is plenty of ammonia and oxygen, it should be fine!

@LK1zt 3 ай бұрын

@@julianliber4633 Thank you for your feedback. Have a good day

@hiteshipant7300 3 ай бұрын

Thankyou sir ❤

@pietervandijk9480 4 ай бұрын

Is there a need to (pre)mordant, or is there enough tannins in the lichen? If there is a need for premordant, what would give the best effect for purple?

@julianliber4633 4 ай бұрын

No need to pre-mordant. Not sure of the exact reason, but the dye will bind without a mordant present.

@christineg8151 4 ай бұрын

Gorgeous! My MIL was cleaning graves on Memorial Day this year and i asked her to save any lichen she found. I was expecting some of the xanthoria lichens because she described it as bright yellow-green, but instead she brought back nearly a quart of this! Looking forward to trying it out.

@KleverskoyeFarm 5 ай бұрын

you should have a rack under the glass jar - like they do with canning.

@delynndehardt1859 5 ай бұрын

It is a beautiful color, but you'd have to collect & use a LOT of lichen for just 1 garment.

@julianliber4633 5 ай бұрын

After a decent windstorm, I was able to collect enough lichen to fill a 1 L jar, which then provided dye for about 400 or 500 g of wool. It will take time, but the lichen gives more pigment than expected!

@jeanstevens2305 6 ай бұрын

GREAT ! You show us pictures of the lichen and how to test it.

@naturalwitchery 7 ай бұрын

GREAT video!

@jatinhemnani6111 7 ай бұрын

How do u prevent scraping agar while removing mycelium from plate The fungi im working on has very flat colony

@merrivideo 2 ай бұрын

I'm having the same issue. How did you solve it?

@high.on.matcha 7 ай бұрын

great video

@caucasianrugs 7 ай бұрын

is it lightfast?

@julianliber4633 7 ай бұрын

I've noticed fading after regular wear in sunny weather after about 6-8 weeks. The color becomes less intense, but retains its hue.

@ellenmorris6915 7 ай бұрын

Last summer, I made this according to your directions and it worked great! Trying it again and after three weeks I have what looks like grape juice colored solution. My question is: How do I know when it's ready?

@julianliber4633 7 ай бұрын

Awesome! I think the longer you let it go, the deeper the color for a given amount of liquid. I'm also not sure at which point the lichen is exhausted.

@ellenmorris6915 7 ай бұрын

(This is a new batch from lichen gathered over the summer.)

@julianliber4633 7 ай бұрын

@@ellenmorris6915 if it's like a deep purple grape juice, then you're probably ready to go!

@linhtrang6456 8 ай бұрын

Thank you Julian

@hanavesela5884 8 ай бұрын

This is amazing. I never heard of this process. Thanks for sharing.

@svetlananana9609 8 ай бұрын

💜💜💜💜Yaaay I love the color purple and almost exclusively wear only that. Thank you:) am exciting to go through the process soon

@jefflangat6052 8 ай бұрын

No enzymatic digestion, no incubatiom on water bath. Just addind ctab and cloroform and thats it????. I doubt it

@kapilramar2253 8 ай бұрын

Which kind of malt extract use Diastatic or non diastatic

@julianliber4633 8 ай бұрын

It's just being used for sugars and will be autoclaved, so it doesn't matter. However, I use dry bacteriological malt extract which is presumably non-diastatic due to heat treatment.

@SilencedByYoutube 9 ай бұрын

I'm pouring in a still air box. Can I leave the lids off the same way until the agar cools or will there be a risk of contamination? I don't have a flow hood.

@julianliber4633 9 ай бұрын

You should keep the lids on in that case. There will be more condensation, but it's not a problem.

@SilencedByYoutube 9 ай бұрын

@@julianliber4633 thanks

@allison5446 9 ай бұрын

I have looked everywhere for a "soup to nuts" method of using lichen as a die and you have done it! I also live on the east coast and I thought that the lichen that I had collected was the common Green Shield (Flavoparmelia caperata) but your instructions on using a drop of bleach verified what I had initially thought was Punctelia rudecta. Yay! Purples!!! I love your step by step directions from harvesting, removal from bark, and the beginning to the end of the dying process. Thank you so much for an incredibly informative video! A++ !

@lisascenic 9 ай бұрын

What an astonishing color! Is it colorfast?

@sandrascotland3639 7 ай бұрын

That's what I want to know

@shirinpayizova8768 10 ай бұрын

Awesome, thank you for informationz

@onceuponatablecloth 10 ай бұрын

I have a question. I started fermenting lichen and now realize I have so much more than I need. Will it keep longer than 6 months if I leave it in the jar? If I want to keep it longer should I strain out the lichen and boil the dye before saving it for later. Thanks for the info in the video so far. My wood pile was covered in the stuff and I got a bit overexcited, which is why I now have way more dye than I need at a time.

@julianliber4633 10 ай бұрын

I've had my jars for 3 years now, still works great!

@onceuponatablecloth 10 ай бұрын

did you strain out the lichen, or just leave it as is? @@julianliber4633

@jorgecarrascof.2025 10 ай бұрын

Hi Julian. I have had failed DNA extractions. Using the lysis buffer composed 100 mM Tris, 250 mM KCl, and 10 mM disodium EDTA (pH 9.5). The samples in tube PCR to 95°C for 10 min and then mixed with 40 ul BSA 3%. I tested in a electrophoresis the DNA and apparently there is no DNA and in PCR I can´t see amplicons. The fungal tested in my case were Beauveria, Metarhizium, Diplodia and Clonostachys. Considering your experience what is the probably problem ??

@julianliber4633 10 ай бұрын

Hi, you can't usually see the DNA on a gel prior to PCR due to the low concentration. How do you do your PCR? I have success using 0.5 ul of template in a 10 ul rxn, with GoTaq green master mix, primers ITS1f & LR3 at 500 nM, and a cycle of 95C/3min, 35x(95C/30s, 57C/30s, 72C/90s), 72C/7min. I've seen that too few cycles can result in failure to see PCR products.

@jeancastro1600 10 ай бұрын

Thanks for the protocol. Could you mention the code of the BSA solution you used or did you prepare it from powder?

@julianliber4633 10 ай бұрын

I prepared from powder, and the protocol has worked in both labs I have worked in so the supplier probably isn't important.

@jeancastro1600 10 ай бұрын

@@julianliber4633 Thanks for the quick reply, could you please indicate whether you used PBS? did you sterilize the solution and how; did you use previously sterilized PBS and then dissolve the BSA? Thanks

@julianliber4633 10 ай бұрын

@@jeancastro1600 because the first solution is buffered, I dissolve the BSA in PCR-grade water then filter sterilize with a low-binding 0.22 um filter.

@matiasguerrap.5456 10 ай бұрын

@@julianliber4633What was the pH of BSA?

@denisemottley22705 Жыл бұрын

Very beautiful shade. 💜

@Medietos Жыл бұрын

Beautiful! Is it the Stone-Lichen I used to make a beautiful rich reddish-Brown colour, with chemicals Alun and vinsten (Sw)? For this fine purple: No further chemicals needed for permanence?

@julianliber4633 Жыл бұрын

No mordant is needed! You can get the color with the lichen dye alone.

@PromorteD Жыл бұрын

Very useful thank you. Confirmed that all of my seeds were indeed unviable!!

@FranOnTheEdge Жыл бұрын

Would it be possible to make ink with lichen like this? And if so must it smell of ammonia? How would you turn it into an ink?

@julianliber4633 Жыл бұрын

It seems like French Purple is made this way. There's a comment that explains more.

@momentobooks Жыл бұрын

I collected lots of lichen on fallen trees on my propety, and it had been soaking in water and ammonia for 2 months now, and no sign of purple at the moment. The liquid is dark brown with some dark red hues, but it yields some uninteresting brownish dirty tone. Do you think it will really turn purple in a couple more months or it should have already started to look like purple is on the way?

@julianliber4633 Жыл бұрын

It's possible that it will turn purple, which happens faster with oxidation. Do you open the jar for air?

@momentobooks Жыл бұрын

@@julianliber4633 Yes, once in a while but I will do it more often to see if it changes anything. Thanks!

@julianliber4633 Жыл бұрын

@@momentobooks it's also possible that it might not be a lecanoric acid containing lichen. If you have some more of it you can try to scratch off the outer layer to reveal the white cortex, then put a drop of concentrated bleach on that spot. If it turns red, it probably is a lichen that will work to make the dye.

@momentobooks Жыл бұрын

@@julianliber4633 Oh! this would explain it! I put a couple different lichen in the jar.

@mdhasibulhossain6389 Жыл бұрын

Can you please share any reference of this protocol? I want to cite this method. Thanks.

@julianliber4633 Жыл бұрын

You can cite this paper: peerj.com/articles/12701 Liber JA, Minier DH, Stouffer-Hopkins A, Van Wyk J, Longley R, Bonito G. Maple and hickory leaf litter fungal communities reflect pre-senescent leaf communities. PeerJ. 2022 Jan 27;10:e12701. doi: 10.7717/peerj.12701. PMID: 35127279; PMCID: PMC8801177.

@ZygoArachnid Жыл бұрын

Thank you for this tutorial! It was very fascinating!

@shirellejohnson1387 Жыл бұрын

I wonder if it would work as a hair tones

@julianliber4633 Жыл бұрын

L'Oreal has a patent, so probably! patents.google.com/patent/FR2907005A1/en

@miwamo Жыл бұрын

Thank you for this video! I‘ve never seen such a interesting color dyeing idea from lichen. Also it’s interesting that you use to gather lichen chopsticks and a tofu package and cook it with a rice cooker😊

@nz-nz Жыл бұрын

How interesting! Would be great to know how this dye was “discovered” (I.e. using the ammonia to extract the pigment).

@julianliber4633 Жыл бұрын

It's been around for a while! First urine was used, then ammonia later. en.m.wikipedia.org/wiki/Orcein

@TrudySchwartzBurrill Жыл бұрын

If you use any acid like citric acid it will be pink or red, is that what you said? I couldn’t hear exactly. Thanks.

@julianliber4633 Жыл бұрын

Yes, you could use citric acid or vinegar to lower the pH and get a red to pink color. The color changes if you wash the fiber in a different pH solution.

@TrudySchwartzBurrill Жыл бұрын

@@julianliber4633 Is that the same chemically as a plant like a hydrangea bush can grow blue flowers in low PH soil the same plant if put with high PH soil will grow pink flowers?

@julianliber4633 Жыл бұрын

@@TrudySchwartzBurrill it's similar, in that pH affects color, but the compound is different. Many pigments (natural and synthetic) are pH sensitive, and can be used as indicators. en.m.wikipedia.org/wiki/PH_indicator

@TrudySchwartzBurrill Жыл бұрын

@@julianliber4633 thank you for the link and information. 👍