Confocal Laser Scanning Microscopy of Arabidopsis thaliana roots - Bonito Lab Training

[@KhanapeenaorGupShup]

Hello, thank you for this video! can you help me with the Propidium iodide staining of roots elongation zone of arabidopsis. I would be great if you could make a video for this. thank you

[@julianliber4633]

You're welcome! I don't work much with roots anymore, but propidium iodide staining can be done with the same method. The staining occurs within 10 minutes of submerging in 10 ug/mL PI. This page may be helpful: sites.duke.edu/benfey/protocols/confocal-imaging-of-roots/#:~:text=PI%20Staining&text=Membrane%20permeation%20allows%20PI%20to,staining%20solution%20or%20distilled%20water.