@Bioinformatics Coach, great job. Just a detail about scaling, you should only fit_transform the training set (X_train) and just apply the scaling to the test set through transform. Corrected code is as below (only the last line has been corrected): # Scale data between 0 and 1 min_max_scaler = MinMaxScaler() X_train_norm = min_max_scaler.fit_transform(X_train) X_test_norm = min_max_scaler.transform(X_test)

You are a blessing. THANK YOU SO MUCH FOR THIS!!

beautiful presentation

wow great explanation .Thank you for your great contribution .This vedio will help me a lot

frankly, how is any of this is simpler than BASH ?

This was very helpful. Thank you!

sir, you are the best

Very helpful

Where did your terminal pop up from? I tried using the Windows terminal on my computer but it does not recognize some of the instructions in use such as pip or wc. Can you please shed some light on this?

What a wonderful tutorial, sir what about installing Python 3.11

Is obtaining controlled data a free or paid process? I'm stuck at sam.gov registration to get UEI. Can you help me with that?

Great job!!! Thanks!!!

hey can we download fastq files using nextflow pipeline only ? so that we do not hv to download on our own

Which software terminal did you use use to read the fasta file? Like cocalc or what?

why use absolute paths in your scripts instead of relative paths?

Thanks bro

Hi! Thanks for the video. I am not sure if you are active, but I was wondering if you know how to split VCFs based on population. I am trying to subset the chromosome 17 VCF from the 1000 genome project, and calculate the allele frequency for each population. But every time I do that, it results in the same data for every population VCFs.

Which featureCounts version are you using? I am getting error for unknown parameter --countReadPairs

Nice, thanks🎉

Best tutorial on this topic I have seen

Nice work 👍

source activate venv worked for me... Thanks

Thanks a lot

worked

When searching for the same accession number in the ENA browser, it shows a FASTQ file, but in your tutorial, it indicates a FASTA file. Could you please guide me on how to obtain a FASTA file for AMR analysis? I would appreciate it if you could provide further clarification on this matter.

Thank you for sharing

you saved my DAY!!!

Thanks for the video! Short and direct to the point!

how can I remove ensembl version number in my data

I'm trying to set the `word_size` to 600, but I keep encountering an error that says the maximum allowable `word_size` for BLASTN is 100. I'm using the same dataset as you, but it seems that this limit is enforced by BLAST itself. Could you advise me on what to do in this case? How can I work around this limitation?

Thank you bro. The video is informative. Can we use the same codes for other bacteria?

I was watching the tutorial can i get the example data and codes that you used.

❤❤❤❤ Very informative and awesome!

Thanks I found it helpful

Great video. I can't find the extract_splice_sites.py file. Could you help me, please?

Please give a tutorial on how to build a database in snpeff also. Please

When I downloading the database. The output doesn't show 'agy99_gca_000013925'. What to do.

Hello sir, can you please make a tutorial video on how to analyse microarray affymetrix data using Limma on R

lots of love and respect from INDIA sir

Dear Sir, I had downloaded codonw in linux according to your uploaded video but it is not working. Can you please help me regarding this. This will help me a lot in bioinformatic. I m waiting for your reply. Thank you

I would like to watch the full tutorial. But your accent it too hard to get. Sorry

Thank you for your video, it has been very beneficial to me. May I ask, I am now using vcftools for pi or fst analysis, how should I set the window size and step size?

Great tutorial! Really easy to follow for someone like me who had never used Python before

hi Coach, I met with this issue: FATAL: while extracting /home/lyhe/.singularity/cache/oci-tmp/7ddae9be99ba04db37484ae3ac2f99a02f6c03bf79b214a6f1d5336dacae4763: root filesystem extraction failed: extract command failed: ERROR : Failed to create user namespace: user namespace disabled. How can I solve this, please

hi Coach, I met with this issue: FATAL: while extracting /home/lyhe/.singularity/cache/oci-tmp/7ddae9be99ba04db37484ae3ac2f99a02f6c03bf79b214a6f1d5336dacae4763: root filesystem extraction failed: extract command failed: ERROR : Failed to create user namespace: user namespace disabled. How can I solve this, please

Sanford Port

Stamm Junction

What if the data doesnt have a pvalue or logfold

Hi sir, Besides this DESeq2 package, which can be used for gene expression analysis, I am asking you due to my version of R studio does not have such package

Answers 1. 13 nucleotids 2. GC percentage= 46.15384615384615 3. purines= 9 4.Pyriminidines= 4 5.Percentage of Purines= 69.23076923076923