Sequence Repeat Identification and Visualization using BLAST and Circos for Bioinformatics

[@desaishailesh3527]

Do you have a full tutorial for de novo assembly for vertebrate animal

[@caglagucdemir5882]

I'm trying to set the `word_size` to 600, but I keep encountering an error that says the maximum allowable `word_size` for BLASTN is 100. I'm using the same dataset as you, but it seems that this limit is enforced by BLAST itself. Could you advise me on what to do in this case? How can I work around this limitation?

[@bioinformaticscoach]

Since BLAST indicates it has a limit. That means you cannot go beyond it because that is a feature of the tool. For a work around, I am yet to find one. So I suggest you stick to the limit. Is there any reason why you want to use 600?

[@caglagucdemir5882]

@@bioinformaticscoach No, actually. I'm new to Circos and currently working through tutorials to learn the software. When I lower the `word_size` to 100 (BLAST max limit), I encounter an issue with Circos, where it tells me that the maximum number of links is 25,000 and drawing that many links (51,650) may create an image that is too busy and uninterpretable.

[@kiranpatil2924]

Hi Vincent How do i contact you,, I sent request in LinkedIn

[@bioinformaticscoach]

You can send me an email : [e-mail]