What about renoving the neutralized trypsin and adding fresh media ?

This helped me alot! Thank you maam ! ❤ may you live a long and meaningful life ❤❤❤😊

you just saved my life

clear explanation indeed .

what the cringe I just watch

thank you doctor, good expanation!

I think the answer to the question will be 25% in Pre-G1 (Apoptotic), 25% in S phase, and 50% in G2/M phases. Because, during the treatment time, the are two equivalent time-periods for the cell death. If the total cell cycle period lasts 24 hours, cells that have at least passed 18th hour and had only 6 hours left to finish the cell cycle will undergo apoptosis (which corresponds to quarter of all cells). Within 12 hours, all G1 phase cells will be at S or G2-M phases, which substitutes previous S phase cells and their remain as a quarter. Another quarter goes to G2-M phase coming from the G1 phase. Lastly, the remaining 25% will also be in G2-M phase (particularly in M phase) due to arrest which came from the 12-18th hours in the beginning of the experiment but did not die.

Bilkent - Cell Biology II - salutes to Onur Hoca :)

really helped in my campbell biology exercise

Learning question about E2F1 and 7/8. If E2F is getting activated via PI3K-Akt-Signaling (due to contact with ionized LNP) and you see E2F8 1.5 fold up but E2F1 not supressed: Should this happen? As far as I understood: E2F8 acts as supressor of E2F1 especially when triggered by Akt-pathway?

Awesome 👌

Thank you Doctor Rosas for your immense contribution to explaining flowcytometric analysis 👍

Thanks for the information

👍

well prepared and clear

A̺m̺a̺z̺i̺n̺g̺ v̺i̺d̺e̺o̺ ❤

Thank you <3

Thank you so much 🙏🙏🙏❤❤❤❤❤❤❤❤❤❤

Thank you very much for this teaching..

Many thanks for your information. I'm curious about how can we record the video cell proliferation process in the incubator?

Thanks sir for such beautiful explanation!👍

Such an underrated video

Thanks!!!!!!

How many cells per well if we use a 96 plate? 72h treatment... I know it varies, but my cells grow a lot, I wonder what you would suggest haha

Great idea, Please keep on this good work.

THANK YOUUU this is so clear and helpful

Great video

Maam , during gram staining , accidentally Cristal violet fall on my hand , I am searching Google about Cristal violet , it show me Cristal violet has carcinogenic and mutagenic ability .Now I am scared can you tell me I am in risk or not 😢

This is the video I needed to see! Thank you Dr Rosas!

I think that the answer is: 25% apoptotic, 8.3% G1/G0, 25% S, and 41.7% G2/M

How can we count the cell?? In cell cycle

This is barely high school level. It would be great to have something for uni students

Thank you Dr. Rosas Acosta! 😳

Great teacher

Thank you for making this video! it helps a lot! great quality:)

Thank you dr Roase The video is very helpful ❤

Fantastic video, you’re a gifted speaker

But DMSO in cell culture causes cytotoxicity in comparison to cells with no DMSO or treatment as a control too.

Promo SM

which cell line u used in this video?

Tysm for the video❤

Took MCB and Cancer Bio with these two gentlemen. My favorite classes during my undergrad at UTEP!

TYSM for making this video. I'm currently applying for entry-level positions in biotech and this is one of the most sought-after techniques. This video is helping me identify the similarities between the cell culture I do now mammalian.

is possible make this cell cycle in algaes from corals symbiodinium??

This professor was my favorite professor and made the greatest impact on my life. I really wouldn't be the scientist I am today without his guidance and teaching.

You are a hero to me, thank you.

Why are we degrading RNA during cell cycle analysis?

Great video! Very informative and easy to understand

To accelerate wound healing a no contact dressing works well. It is called Podophylus antimicrobial Insole and DermaDomus, both allow oxygen in the wound…