When we have any proplems, there will be an Indian guy will explain those problems for you. Thank you this help me a lot!

very helpful, thank you

The good thing about polyplus transfection reagent is that you can directly add it to the serum containing media when the cells are confluent.

Brother i am working on knockdown studies, polyplus transfection reagent has higher efficiency than lipofectamine

Excellent content ! Dude but please organise it in proper playlist(s), that way you will get a broader reach. Thank you

Thank you for explaining, it is so clear, I have question: when you loading the sample to western blot, did you loading it to times? Like why there are two bands in the gel??

This was extremely helpful! Thanks for this great explanation!😀

Hi sir these is suresh PhD student from south india , if possible please share the software link its use full for my phd work sir

Please provide software name

❤️❤️

Thank you, I am going to do the assay tomorrow

Can yu suggest more tools for codon bias analysis

Should we put a break in the first centrifugation?

How to Avoid RBC contamination in pellet? We use fresh samples for pbmc isolation. But still we get RBC mixed with cells in pellet We will use 15 ml both falcon and sepmate tube. Centrifuge 1st step we do with breaks

do we need to change media every day??

Thanks so much 👍

Legend

Hi, on western blot visually 5 and 10 ug protein band is more intense compared to control but after normalization on the graph it shows control is higher amount compared to two other bands, why?

Can you plz help me out to design sequencing primers to confirm the mutations?

Dude, you should have shown the full alignment and exporting steps

Hi, As the scFv has the lower affinity, do you have any idea to enhance the affinity from the plasmid construct? Thanks

You video is so explicit and detail. I did the exact alignment just by watching. Thanks for sharing.

Sir really appreciate your efforts

Really appreciate

Its really useful ❤

❤

So thankful for this video. If my bands have some background how do I remove it? and one more question, if I have 3 drugs group and 12 samples total, what would be the control here?

Did you already try to overlap PCR for 2 fragments with high GC content. I try to overlap PCR with fragment A is 1700bp, 65%GC, fragment B is 790bp. My overlap region is P2A with 30bp. Product size of overlap PCR is around 2600bp. But when I run gel electrophoresis, I cannot see the band of product, and I don't know why?

Its paid ;-;

can you please make videos on how to make primer sequence for genes , for performing rtpcr

Thank you very much, amazing presentation, very helpful. I am going to do tumor xenograft of acute myeloid leukemia. T

This video is so helpfull. Thank you so so much

Why did you choose to test only when you don’t know whether it is normal or not normal before choosing the test

thank you sir

Your explanation is mostly correct, expect for the OD being related to the protein expression. In that example you explained is a way to measure the amount of bacteria in the medium. We only add the IPTG when the OD is high (=lots of bacteria are there, around OD=7) and in your examplary images they will keep multiplying during the protein expression to an OD of 1. Also, your images are really small and the stuff you wrote in the red letter (6:00, f.e.) is not easy not read.

Thanks so muchhhhhh

Thank you so much sir 🙏

Thank you. Highly appreciable that you took your time and responded to our queries. It was really helpful. 😊🌸

Your explanations are best

Hello, sir. Can you please explain how to quantify in an excel file and graph plotting. Also, how to normalize it with loading control.

Sir very useful video thanks .

When does this methylation of the parent DNA occur? How did the parent DNA get methylated?

"Promo sm" ☀️

Excellent. Helpful video.

Helpful video thank you!

When I try to display a 2D diagram of the ligand in Discovery Studio, I receive an error message saying 'Ligand is not a single fragment' and I don't know what I need to do. I need help to resolve this issue.

Hii sir Thank u so much for sharing. Usually i scare ti study biochemistry.the way ur explained is simply superb. Pls do a video on contracon heracell 150i.it vil helpful for me. U know if anything i have to do independently I vil take decisions,theory class first.then i vil implement. Once again heartful thanks to u. For good video

How to make graph after getting mean of the all groups. ?

I have a question. In the case of antigen in the sample where you first added the antibody and it dried up, How do you achieve a control for that. Because since the antibody is loaded on to the membrane first. Won’t it bind the secondary antibody regardless of the binding to antigen. Also what’s the ratio of background when done ??

I’ve always had this question but no one seemed to know the answer, I now know why the DPN1 restriction enzyme cleaves only the parent strand but not the amplified strand. Truly one of the questions of all time.