Join multiple DNA fragments ll Overlap PCR ll Xgene & proteinX

[@TheNyakaat]

Thanks for the effort but when you got to the actual overlap PCR part, you didn’t really explain clearly how it should be done.

[@xgeneproteinx2889]

Thanks. I got your point. If you have any confusion to understand clearly, you can write it down. I will try to explain it.

[@kcampbell9248]

hello thank you for the video. I need help with this question please: How would you use PCR-amplified oligonucleotide directed mutagenesis to create deletion of amino acids 75-83 in subtilisin or insertion of the amino acids glycine- glycine-glycine between amino acids 74 and 75 of subtilisin@@xgeneproteinx2889