Thanks a lot to give this presentation!

How do i import images on cellprofiler and how can i get a software that works on windows 7

Thanks a lot. What would happen if instead of putting 5% PhiX in the cartridge, I mistakenly put more amount (60%). And I put the correct amount from the library?

amazing lifesaver

Thanks so much! Even the illumina webinar was not as clear as your presentation! 👏👏👏👏

Thank you for the great explaination!

this is great!

Is it safe then to assume that molecules that divide would be the point at which inorganic becomes organic and chemistry becomes biology?

Thanks. Can you clarify at 8:04 when you said each beads has many barcoded oligo-dT oligos on its surface. What is the scale of this "many"? Millions? Are these supposed to attach to the entire transcriptome of a cell?

17:13 quantifying proteins using DNA sequencing (CITE-Seq) 21:26 universal antibodies for a universal cell-surface protein that have unique barcodes for each cell to detect the unwanted doublets (droplet with one bead but two cells)

Awesome

What is the purpose of waving your hands?

Please don't wave your hands--it is very distracting

Amazing overview Excellent

Nice talk!

Very detailed and clear! No ads interrupting! Thank you very much professor!

Thank you! It helped me a lot

I looking for good videos in how analyzing the a dataset of Peripheral Blood Mononuclear Cells (PBMC) ?? Thank you

fourier transfer of fourier himself is such a nerd thing to do lol, love this lecture Thanks!

Great lecture!

Fantastic video! You explain very well

روى ابن ماجه وغيره أن النبي صلى الله عليه وسلم قال: إن مما يلحق المؤمن من عمله وحسناته بعد موته علما علمه ونشره، وولدا صالحا تركه، أو مصحفا ورثه، أو مسجدا بناه، أو بيتا لابن السبيل بناه، أو نهرا أجراه، أو صدقة أخرجها من ماله في صحته وحياته تلحقه بعد موته. m.kzbin.info/www/bejne/Y6LKpIKsrNF-Ztk&pp=ygUg2KfZhdis2KfYr9mG2Kcg2LnZhdix2Ygg2YXYrNiv2Yk%3D

Thanks for this great presentation!

how does this not have more views

Best lecture

Great stuff, will be featuring you in our next episode!

If you are really interested on, u can dive in Jost 2018 (measuring differentiation). He argue about the limitations of Fst and its relative statistics (yes, there's a lot). You will see that comparing expected heterozygosity as a proxy of differentiation might fail at the populational level in several occasions. For instance, it can only reaches 1 if there are no allelic sharing, and put 11% of differentiation when there are 35% of allelic differences because it rely on the average of a possible heterozygosity that might comes from a loci in case of panmixing breeding (a lot of assumptions). Jost argue that it would be more helpful try to compare the frequency of alleles, mainly for those loci (or markers) that can measure a multialellic state ( more than two, usually). Fst still usefull, but you can't take that as the ultimate tool to measure everything. Remember, a 30 inch ruler is useless to measure the distance between the sun and the earth or the circumference of a cell.

Great video and great information

Great to hear

talking bullshit

Nice work

i have a question? How to determine the position of the optical axis at the microscope imaging plot? Because I need an external optical path

Thank you so much for this, Eric! Very easy to understand.

In 10 years no one’s made a single comment? Sorry, Tony. RIP Sydney and John 😢

unsharp images for much money

I absolutely loved this lecture. Kinda brings into context how vastly complicated the whole biological system is. This is so beautiful.

This video is gold. Thankyou for this! Recently this tech (CAR Tcells to attack cancer) was in news, in India. This is so cool.

Super nice explanatory video! Thank you!

Sir, I apologize but you still are not defying it in laymen terms that average person can understand.

You're an angel Ron!!!

Thank you sir, for your tremendous videos.

I kinda have a celebrity crush on her NGL

Amazing, thank you

First

Great explanation!!

This dude is as boring as an old man's diaper!

one size FITC all

Dang! That was fascinating and scarily understandable, thank you!!!

Wieso muss denn beim Koehlern der Kondensor zentriert werden. Seine optisch Achse sollte doch in jedem Fall mit der optischen Achse des Objektives uebereinstimmen und nicht mehr veraendert werden. Wohl aber sollte die Leuchtfeldblende zentrierbar sein. Das bedingt auch immer eine zentrierbare Punktlichtquelle.

Amazing and very detailed explanation. KZbin is so great