Single Cell Sequencing - Eric Chow (UCSF)

[@Keigan884]

Please get Eric to do more of these videos, this is by far the best overview, and easiest to understand, that I’ve found. He’s so good at actually explaining in a way that is not overly technical on purpose. I feel that many in the field are gatekeeping their knowledge, instead of explaining in ways that others can understand.

[@12823matthewkao]

right....i feel researchers nowadays are reluctant to teach students technical skills and overly focusing on theory and critical thinking....thats why they need to import tons of post docs from china and India because American student are lacking technical skills!! this is really messed up!

[@francescoprevedello8658]

I think like its more that most people on the field dont actually know that well how every aspect of ngs work, since it is a process that involves tons of different expertise, and each one learn well mostly what they need to know. The flow process to obtain a diagnosis from a sample boood requires several different experts in chemistry, phisiscs, biology, biotechnology, medicine, engineering, bioinformatics, therefore it is difficult to find someone who actually has the knowledge to perform a well explained overview of the process. That is why the prof. In the video is so talented

[@esan120au]

17:13 quantifying proteins using DNA sequencing (CITE-Seq) 21:26 universal antibodies for a universal cell-surface protein that have unique barcodes for each cell to detect the unwanted doublets (droplet with one bead but two cells)

[@anastasiapivnyuk6985]

Really cool! Thank you Dr. Chow for this detailed and at the same time clearly structured talk!

[@merrimac1]

Thank you for the nice introduction of single cell sequencing methods!

[@BasicScienceSeries]

Thank you Dr. Eric for this wonderful talk. You have provided detailed information on Single Cell Sequencing and explained every detail of this technique. I found the lecture valuable and highly informative. Thank you very much iBiology Techniques for providing this valuable lecture.

[@codewithluq]

This lecture is highly informative and greatly valuable. It has cleared most of my gray areas in the field of single cell analysis

[@je11y_jju]

Thank you so much for this introduction of sc-RNA Seq. This video helps me a lot to understand each steps even though I didn't have much of understanding about this field. Thank you again.

[@jordanramilowski9413]

You look for something on KZbin. The video with Eric Chow pops up. You know its gonna be good...

[@karimali5850]

One of the best scSeq overviews out there. Keep it up you're doing great!

[@captainquinine]

Excellent talk

[@anjadekanski3879]

These sequencing videos are really great! I hope you can also cover UMIs, spatial transcriptomics, and other such difficult concepts :)

[@marcuslcc5257]

Extemely helpful for newbies!!! Thank you Dr. Chow!!!

[@samanwoymukherjee4945]

Excellent talk and lucid explanation of the biology Dr. Chow. Thanks iBiology, looking forward for such talks in future.

[@meenukesarwani8665]

Dr Chow I love your videos. Please continue your good work. Its really thorough and well explained with theory behind each technique which are not explained from the product maker /companies. Thank You!

[@AliRazaShahSARSB]

Thank you, It was really good to understand the basics of single cell sequencing methods.

[@mistarelentless5722]

Dr. Chow you is da G.O.A.T. grateful!

[@beckysam3913]

Thank you for this coherent and excellent introduction!

[@catherinegrgicak9570]

Great presentation! Just great! I agree with all of the other positive comments. The content is great, easy to follow and Eric is a great presenter.

[@bhartithakur2748]

Thanks for this amazing video Dr Chow

[@esan120au]

Thanks. Can you clarify at 8:04 when you said each beads has many barcoded oligo-dT oligos on its surface. What is the scale of this "many"? Millions? Are these supposed to attach to the entire transcriptome of a cell?

[@qutemango]

thank you for explaining these technologies so clearly! it's very useful for me.

[@rainajung185]

Love Eric’s videos so easy to understand!

[@gonzalovillanuevamartin9807]

Amazing video, amazing explication. Thank you Dr. Chow!

[@panther25423]

Fantastic video.

[@aatt3209]

Excellent overview summarizing development up to 2019 in this developing field. Thank you for your hard work putting this presentation together!!

[@gnosisdataanalysis8595]

Bravo! Great video. Single Cell - clearly expained!

[@여가뮤직프로듀싱]

Thank you so much for this lovely talk. I think this is the best talk summarizing scRNAseq.

[@deeptimittal4552]

Nice introduction. Kudos

[@valentynapolishchuk1123]

Great video, thank you! Very informative and easy to understand!

[@kaiqin5306]

wonderful lecture

[@vikas3141]

well mam from last few weeks ,I am focusing on biochemistry portion ,althrough I read all content of book but I'm not satisfied with that content , can you please answer or make vedio on my following queries: (1) in lab whenever we isolate some compound and want know the structure of compound ,how do we really formulate them , what are those techniques , are these techniques 100%accurate or just give approx idea For example , I always wonder how scientist who has first isolated ATP molecule , how he give structure of ATP , infact how he isolate compound at level of molecule, (since i have isolate compound at level dna only) how cycle such as kreb cycle or glycolysis are performed in lab Your entire vedio lecture have always motivated me , thanks a lot I am from india

[@jessicalv6442]

Thank you so much!

[@AA-gl1dr]

Excellent explanation thank you so much!!

[@azrahasan3796]

Great video. I got a question, In demuxlet. If you are working with different individuals, the possibility of two different cell types from same individual is not that rare. How they differentiated the cell population from same patients.

[@ye2e2]

Thanks for the insightful sharing.

[@a3m9b]

Thanks for the explanation! Very helpful for my course

[@qsc9546]

What's the overall accuracy of Seurat and Scanpy? Is that significant?

[@deepikachaudhary-ge5cj]

nicely explained....

[@OsamaElghawil]

I looking for good videos in how analyzing the a dataset of Peripheral Blood Mononuclear Cells (PBMC) ?? Thank you

[@sarowerbhuyain4905]

Just splendid!!

[@extropiantranshuman]

not one dislike - exactly! Love data visualized in a 3D space!

[@kabriyacharlest9018]

Thank you Dr. Chow for that! That was explain so well, so much detail but you explained it in a way that was so easy to understand this! Such a great presentation!

[@tigerkeys6406]

Really cool what is the tool you use to record this video that you can embed into the video?

[@JoseAntonioOU4]

Thank you for the lecture. I need to go back to school. It would have been useful to include costs/instruments

[@disturbingdevelopment4308]

Why can't the doublet microparticles be distinguished from singletons using light scattering or fluorophore techniques?

[@qsc9546]

How's performance of supervised methods? Any pitfall?

[@qsc9546]

What is the influence of clustering on downstream analysis? Differential expressed gene?

[@search_is_mouse]

thank you!!!!!!!SO MUCH!!!!

[@ishapandey28]

Can you please explain what microfluidic is?

[@qsc9546]

Is clustering the bottleneck of scRNA-Seq or the doublelets. Does 0.6 clustering accuracy mean 0.4 doublelets?

[@SanchoColins]

Brilliant.

[@lovelysmart97]

Thx for watching!

[@ODjangoo]

as of 2020, approximately how long does it take to run scRNA-seq in a single sample?

[@sofiakathiria7050]

atleast 2 days

[@ODjangoo]

@@sofiakathiria7050 thanks, someone told me 1 day and i was very skeptical :)

[@onefuture6]

I love your voice.

[@simonsays5094]

Thank you sir this was very helpful

[@HuongLe-li2wy]

Some segments in the video are stamped not adjacent to each other

[@edthoreum7625]

5:00 first cell sequencing,,,

[@wl2007]

How do they know which cell type the mRNAs came from originally?

[@ricardosantos6554]

Im a bit late, but you atach a specific barcoding RNA to each cell

[@ayitm]

I'm pleasantly surprised that this video has this many views!

[@sleepyowl910]

Lost my appetite for smoothies!

[@tiangechi1144]

How sexy is his voice omg… nearly forgotten what I’m here for.

[@vanderleidasilvafragajunio1197]

I think I found my husband!

[@hraqhraq]

Not a good lecture, unless you are specialized in this field; even not clear for medical students to understand what is he talking about