Very interesting.. i Think it won't be on the public radar.. in most countries... or every public pension system will collapse.. The ideal for a government should be stay healthy until a defined age and then die.. live longer = bankrupt (I obviously assume it won't be possible to quickly increase retirement age for most of the jobs)

Google: GlaxoSmithKline and David Sinclair. You’ll come to know more about David. A lot more.

You are brilliant! Thank you for your research!

On VL's comment about not being a ketogenic diet it nevertheless raises ketones and lowers glucose, alot. I've done the official FMD and my own version based on the macro breakdown and in all case my ketones went up to clinical levels (.0.5 mmol) in the 2 to 3 mmol range by day 3 of 5. I take this as just a marker of low insulin. My glucose went down to 70s from high 80s/low 90s. Highly recommended. But do before and after blood work. And when you come out of transition to a good diet and TRE if you can. I did the FMD as two meals/day about 8 hours apart, so a form of TRE combined with FMD.

Os it just me or did she describe a great leap forward to cure blindness...

In his book, Longo shows how to do a DIY version of the FMD. Very easy. I do it once a year.

Very interesting. Never heard much about IL-11 before.

Thank you so much! Muchas gracias from Mexico!

Muchas gracias, thank you so much

Very interesting talk. Love biology

Vittorio Sebastiano from Turn Biotechnologies presented at the 10th Aging Research and Drug Discovery conference on cellular and tissue rejuvenation through epigenetic reprogramming. The key points from his presentation are: 1. Epigenetic drift, the progressive accumulation of epigenetic errors in the epigenome, is believed to be a fundamental driver of cellular aging and loss of function. Over time, genes that should be off get turned on and vice versa, impacting many hallmarks of aging. However, unlike the genetic code, the epigenome is reversible and can potentially be reset. 2. Inspiration for epigenetic reprogramming comes from the works of John Gurdon and Shinya Yamanaka showing that somatic cells can be reprogrammed into embryonic-like cells through somatic cell nuclear transfer (Gurdon) or induced pluripotent stem cell techniques (Yamanaka). This suggests aged cells could potentially be made more youthful. 3. In 2014, Sebastiano's group proposed using a controlled partial/transient reprogramming approach to change the age of cells without altering cell identity. They published foundational work on this "epigenetic reprogramming of aging" (ERA) in 2020 using a cocktail of 6 factors delivered as mRNAs. Since then, several studies have validated the approach in various tissues (muscle, skin, brain etc.) and organisms (mice, rats, primates). 4. Turn Biotechnologies is working to translate ERA into clinical applications by combining the biological concept, mRNA delivery vehicles, and lipid nanoparticle (LNP) delivery systems. Using mRNA avoids genomic integration risks and allows fine control of factor expression. Certain factors like MECP2 have beneficial effects when pulsed briefly in combination with others, contrary to some misconceptions. 5. In dermatology, ERA shows promising results: - In primary human fibroblasts, it causes transcriptomic resetting with activation of ECM related genes, anti-inflammatory effects etc. Fibroblast identity genes are appropriately modulated. - In human skin biopsies cultured ex vivo, aging biomarkers are favorably altered after ERA treatment. - An in vivo human skin xenograft model was developed to test ERA delivery and efficacy over several weeks. 6. For immunology, ERA is being explored to rejuvenate T cells: - Treated T cells from old donors proliferate robustly in response to tumor cells without any change in cell identity markers. - They express lower levels of exhaustion markers and maintain better anti-tumor cytotoxic function compared to controls. 7. ERA has potential applications in many tissues like muscle, eye, cartilage, blood, and skin, with dermatology and immunology being the initial focus areas. 8. Delivery of mRNA remains a key challenge, especially achieving sufficient penetration. For skin, microneedling/injections are the near-term approach, with topical solutions being developed. Broader interest and investment in mRNA and gene therapies, catalyzed by COVID vaccines, is expected to accelerate progress on novel delivery systems. In conclusion, Sebastiano presented compelling evidence for ERA as a promising approach to partially reverse cellular aging through transient epigenetic reprogramming. By translating ERA into viable clinical therapies, Turn Bio aims to ultimately increase human healthspan. While delivery hurdles remain, the underlying biology appears sound and reproducible across contexts. ERA represents an exciting frontier in the fight against aging and age-related dysfunction.

Doing my first round of FMD. Primarily interested in anti cancer. Wondering if alternating the FMD with a vegan KETO would stress cancer cells more than just FMD. Example, month 1, FMD, month 2 Ketogenic diet (5 days).

Thanks. Very important. As a 69 yo male very encouraging as I exercise daily these last 12 years. Hba1c stays ~ 5% and hsCRP at <= 0.3 mg/L consistently. Will test IL-6 on morning before exercise to confirm it is low.

The research is absolutely stunning and many people are pending from any news regarding this study. However, I think the difference between targeting "degenerating" or "dead" retina cells in the end of the talk, could have worth a bit more time explanation. Also, do you plan a glaucoma model for the non-human primate test?

I've read his book lifespan couple weeks ago.. and thereafter I'm looking for his interviews

If he becomes my teacher, I’d be honored.

That is amazing!! I will like to hear more...l would like also read the book Lifespan!!😊by David!!

Pure NMN powder from donotage is really good.

I would like to know you said this company is going to make NMN Into a drug, how is that going to affect the rest of the people that you are educating on his benefits, to me that would limit it greatly as to who can get it?

I wonder if he has looked at senescent cells? Are they reduced as in senolytic therapies?

So VERY DISAPPOINTED that you are associated with the WEF. As we all know Klaus Schwab, Gates and the WEF are for depopulation of the world by billions!! Unless these supplements are only for the elites I am very hesitant to believe what is presented. Maybe the average "useless eater", as Klaus calls the general population is not supposed to hear this information or take the supplements!

Did I hear him claim to have dead lifted almost 1,000 pounds?? If so can't he hold a stationary Iron Cross on still rings with 100 extra pounds strapped around his waist?

Did he say, penguins are mammals?

Methionine is better then acuall sam-e for creating sam e and having a anti depressive effect new studies show. Yet many years back aging docs pushed that methionine restriction increase lifespan. Wich makes no sense as methionine is a must for methylation and sam e creation.

I want to move to Singapore now.

Seems criminal that I can't make an informed decision by myself to use rapamycin and take the risks: the possible rewards seem worth it. The whole "we need rapalogues cuz pharma isn't interesting in rapamycin cuz it can't be patented is infuriating.

really the one person to ask a question was some girl who wanted to know what to study...great way to waste it...🙄

I can tell he is a toxic PI the way he talks about his student

She seemed very nervous, but she did a great job. Very interesting talk.

I have done the Prolon 5 day fasting mimicking diet and having the prescribed food made it possible to tolerate the diet because extreme hunger wasn't a factor. I believe in Dr. Longo's research.

Love this

Thank you very much for your valuable information ♥👍👍

What is the mechanism to revert age by applying four Yamanaka factors?

A very interesting talk and subject. I'm surprised so few seems to react on it. Only one comment, for example!

A price implies a selective process that perpetuates the cycle of disenfranchisement for those who have no substantial income beyond housing and food.

Wow, that is super-exciting research. Great talk.

Fantastic. Dr.Coray provides comprehensive evidences to prove this old prediction is true.

Bus drive surely could have waited one more question, you have David Sinclair on stage. Come on!

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This was most interesting! The connection between mitocondria and senescence is new to me, and seems to change things compleatly. The similarity between apoptosis and senescence is intriguing! I wonder what effect PhotoBioModulation would have on senescencent cells. Since that affects the mitocondria. Would it push them into apoptosis, into recovery, or neither?

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When was this conference taking palce?

Find it interesting and novel in exploring the over expression of genes, which is a field of biology that has undergone little research until now.

This is huge and not getting enough attention, we need this today not 10 years from now , people are dying and suffering from easily curable diseases , synthetizing Peptoids is not harder to synthetizing than regular peptides .

Can't wait to live to 150 years old so I can sleep all day. That would be epic.

Wait...funeral? I thought the idea was to avoid that one...

I don't know how people do it. I'm lucky if I can fast for half a day. Fortunately, I don't mind exercise and my eating habits are improving. I just love to eat!

He is getting younger and younger,means that he already got that how to stop aging ❤❤❤

This was a super interesting talk. I don't have enough background to follow it in detail, but my take away is that stem cells are pretty fragile and that any disturbances in metabolic balance could accelerate aging, deplete regenerative capacity, and/or lead to disease states. I found it particularly interesting how mitochondrial fusion and fission determine the state of the stem cell. From my other reading I gather that not much is known about all of the the factors that influence mitochondria fusion and fission. I have recently read of insulin sensitivity being implicated in mitochondrial behavior as well as nutrients such as dietary steric acid signaling mitochondria to fuse.

You don't need to learn how to code