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@kshrshrie9718
@kshrshrie9718 7 ай бұрын
what is the concentrations of the chemicals you are using to make hydrogel?
@joshp4582
@joshp4582 9 ай бұрын
One Shudders to imagine the evils to come.
@jayeshparmar1256
@jayeshparmar1256 10 ай бұрын
Hoyaaaaaaa
@ツルラヴァンチェリーサリムファジール
@ツルラヴァンチェリーサリムファジール Жыл бұрын
what are the criteria for selecting the initial guess value (Ka)?
@jaimataki2615
@jaimataki2615 Жыл бұрын
Does that mean physical entanglements??
@pr_wanted_director
@pr_wanted_director 2 жыл бұрын
What is a procedure ?
@reshmajolly5892
@reshmajolly5892 2 жыл бұрын
cAN I GET YOUR EMAIL?.I AM WORKING N HYDROGELS
@phantomcreamer
@phantomcreamer 2 жыл бұрын
How do you run statistics on the sigmoidal curve?
@igerare3745
@igerare3745 3 жыл бұрын
How scale
@AnNguyen-py8tx
@AnNguyen-py8tx 4 жыл бұрын
one more question could i ask that, which are the components of negative control and positive control and untreated wells in your experiment ? thank you
@AnNguyen-py8tx
@AnNguyen-py8tx 4 жыл бұрын
excuse me, i wanna find your protocol, could you show me which is your website ? thank you
@joebill9868
@joebill9868 4 жыл бұрын
fucking geniousssss.. i love you bro. :D
@windturbineusa3641
@windturbineusa3641 4 жыл бұрын
where do you buy "peptide gel' base powder? Is it pH stable in a strong base once it has "ACID GELLED" ??
@RadiumVader
@RadiumVader 4 жыл бұрын
I really appreciate the open source concept and especially the possibility to share raw data and analysis results via a perma link. Could you please explain how the raw data should be prepared (file type, order of values, units etc.) for the IC50 fit? The example file for the titration experiment (association constants) was very helpful.
@RadiumVader
@RadiumVader 4 жыл бұрын
Thank you for your video. Is there any new information about openkinetics.org/ ? It would be amazing to have a reliable tool assisting with the analysis of reaction kinetics, too.
@RadiumVader
@RadiumVader 4 жыл бұрын
Thank you very much for this video and for the tools provided on supramolecular.org. These were extremely helpful for my current research.
@moupiamukherjee6457
@moupiamukherjee6457 4 жыл бұрын
wow... That was like a pocket notebook tips I always read in science stories ... On a serious note, it was really helpful in visualizing the real thing.
@riturajputriturajput4177
@riturajputriturajput4177 5 жыл бұрын
Can u suggest drug for d prep of microsponges
@IOITTIOI
@IOITTIOI 5 жыл бұрын
The movie is really nice and instructive and the website supramolecular.org as well. I have only a minor remark here regarding the value of the host concentration at 0:35 min and guest concentration at 0:55 min. I think that based on the calculation shown the former one should be 0.464 mmol / 0.5976 mL = 0.776 M and the latter one should be 33.32 mmol / 0.33804 mL = 95.6 M.
@pallthordarson4385
@pallthordarson4385 5 жыл бұрын
Thank you. Yes, I discovered there is a typo there. The problem is actually at the top of the slides. Look at 0:35 for instance. It says 1.08 mg / 2320 mg/mmol = 0.464 mmol. Now that should actually be 0.464 micro-mol (0.464 umol)! I must have typed the "micron" symbol but it some converted to "m" without me noticing it. You see, then it all makes sense. At the bottom it would then be 0.464 umol / 0.5976 mL = 0.776 mM (because micron / milli = milli). And then 0.776 mM = 0.000776 M (7.76 x 10^-4 M). Thank again for pointing out this mistake!
@colorsoflife1090
@colorsoflife1090 5 жыл бұрын
Very well explained Dr Adam! It helped me a lot! Thanks.
@colorsoflife1090
@colorsoflife1090 5 жыл бұрын
Really helpful and precise
@dr.mohameda.abdelaleem7894
@dr.mohameda.abdelaleem7894 6 жыл бұрын
Thanks ....... Allah Please you
@pallthordarson4385
@pallthordarson4385 6 жыл бұрын
Cool dad
@ismaelmartinezcortes3743
@ismaelmartinezcortes3743 6 жыл бұрын
Hello Andrew sorry fo rmy question but, which is the quanitity (g) of alamarblue for mL of solution that you used? becuase I did one solution 10% and it was verry saturated and my experimetn fall.
@ajkihn5626
@ajkihn5626 7 жыл бұрын
Hi, I wanna thank you so much for this video, I learned a lot and clarify many doubts when I watched this. Now I feel confident to perform this part of my study. Thank you!
@urvibhatia6563
@urvibhatia6563 7 жыл бұрын
can you please give a detailed data of the experiment you performed and why did you do this by only these methods .?
@pallithordarson4164
@pallithordarson4164 7 жыл бұрын
This flight was with www.airborne-aviation.com.au/ (Airbourne Aviation - Camden, Sydney).
@Ice84letters
@Ice84letters 7 жыл бұрын
un muy Lab they remove the media before adding the mtt and put a fresh media without phenol red. then the mtt, incubate for 2 hours, remove and then they Also add isopropanol instead of dmso, could you give an opinion about this protocol?
@ayeyebrazorf7527
@ayeyebrazorf7527 6 жыл бұрын
Alamar blue versus MTT is 1)cheaper 2)faster 3)more reliable 4) doesn't kill your cells
@amirulfaez8437
@amirulfaez8437 8 жыл бұрын
Why do we need to do serial dilution before plating?
@rashean
@rashean 4 жыл бұрын
so that you can see the effect of the drug on the cells at various concentrations of drug. You can see the effect at high dose, medium dose, low dose and of course 0 dosage.
@unays
@unays 4 жыл бұрын
@@rashean Appreciate