IC50 or cell viability experiment

[@ajkihn5626]

Hi, I wanna thank you so much for this video, I learned a lot and clarify many doubts when I watched this. Now I feel confident to perform this part of my study. Thank you!

[@AnNguyen-py8tx]

one more question could i ask that, which are the components of negative control and positive control and untreated wells in your experiment ? thank you

[@AnNguyen-py8tx]

excuse me, i wanna find your protocol, could you show me which is your website ? thank you

[@phantomcreamer]

How do you run statistics on the sigmoidal curve?

[@ismaelmartinezcortes3743]

Hello Andrew sorry fo rmy question but, which is the quanitity (g) of alamarblue for mL of solution that you used? becuase I did one solution 10% and it was verry saturated and my experimetn fall.

[@Ice84letters]

un muy Lab they remove the media before adding the mtt and put a fresh media without phenol red. then the mtt, incubate for 2 hours, remove and then they Also add isopropanol instead of dmso, could you give an opinion about this protocol?

[@ayeyebrazorf7527]

Alamar blue versus MTT is 1)cheaper 2)faster 3)more reliable 4) doesn't kill your cells

[@amirulfaez8437]

Why do we need to do serial dilution before plating?

[@rashean]

so that you can see the effect of the drug on the cells at various concentrations of drug. You can see the effect at high dose, medium dose, low dose and of course 0 dosage.

[@unays]

@@rashean Appreciate

[@joebill9868]

fucking geniousssss.. i love you bro. :D

[@dr.mohameda.abdelaleem7894]

Thanks ....... Allah Please you