🔬 100 - How to make a specimen slide with WATER LIFE | Microscope

I show you how to prepare and observe water life. Besides algae, I found several single-celled organisms, protists, also Vorticella.
🎈 SUPPORT
Become a Patron: / microbehunter
🔬 MICROSCOPE RECOMMENDATION
I receive many questions on which microscope one should buy. I recommend the following models for amateur microscopy (Affiliate Links to Amazon):
Children’s/Introductory - Swift or National Optical SS110: amzn.to/2WdS4PX
For Amateurs - Swift SW380T (with phototube) or Swift SW380B: amzn.to/3fu7aIY
👜 AMAZON AFFILIATE SHOP
US/World: www.amazon.com/shop/microbehu...
Canada: www.amazon.ca/shop/microbehunter
Germany/Europe: www.amazon.de/shop/microbehunter
UK/Europe: www.amazon.co.uk/shop/microbe...
🎬 KZbin CHANNELS
Microbehunter Microscopy (advice): / microbehunter-microscopy
Microscopic Mysteries - Guess the specimen: / @microscopicmysteries
Microbe Talk - Ask a microbiologist! / @allthingsmicrobes
💻 OTHER LINKS
Main Website - Tons of microscopy info: www.microbehunter.com
MicroWorldArchive - www.microworldarchive.org
Forum - Connect to other microscopists: www.microscopy-forum.com
Instagram - Cool pictures I made: / microbehunter
Facebook - Here I post videos: / microbehunter
✔ Check these Hashtags! #microbehunter #microscopy #microscope

[@valor36az]

Your enthusiasm for your profession is so admirable

[@hungryhippo4560]

I'm fairly certain he's just a hobbyist

[@Mpower20188]

Yes but he’s also a biology teacher :)

[@sportsfails4998]

Hungry Hippo if you spend 5000$ plus on microscopes it’s not just a hobby anymore

[@DabbaHashery]

@@sportsfails4998 I mean unless you're making money from that microscope than yes it's just an expensive hobby.

[@MrInternut]

Thank you for these videos. My undergrad is in bio/zoo but 40 years have gone by since I last peered througha microscope. I have just ( 2 days ago) acquired a decent scope and will be getting back into aquatic invertibrates again...very exciting. So far, all I have done is a couple blood smears and looked at some old invert slides I made back in town1978. The blood was nice (...don't tell my wife...she slept right through the collection process and didnt even budge when I poked her...(just kidding...it was my blood...really;)). The old slides were interesting but many of them were not in good shape...just like me?? The slides were mounted in PVA back then so I will have to review modern slide preps. Anyway, I am really enjoying your videos...thank you!!

[@MaishidaHD]

I love how happy you are about this kind of stuff. Puts a smile on my face every time! 😊

[@DrSoda.]

This is the best channel ever! Thank you for videos.

[@niramaygogate7904]

From next video onward, whenever you find some interesting organism, I would like if you could show it in both light field and dark field ..

[@questionminecrafter]

I think the small dot like things are spores of the algae... Though I'm not sure.

[@SpenserFranco]

I always enjoy your videos! I'm so interested in microscopy and science and biology! You have very informative videos. I love seeing all the tiny life in the world. Keep up the good work! Hi from Arizona!

[@markschofield576]

Great fun as always, could you show us the magnification you are using in the different scenes please?

[@lawriegraney9929]

Hello, Microbehunter. Hope you are well. It's now May 2020, and I've taken up for the third time in my life microscopy. I had a fascinating day under the microscope today. I looked at a running fresh water water source by the sea, which I have been using for a drink of tea after boiling it. Actually, it tastes quite nice and fresh when straight from the stream. It comes from quite an amazing natural waterfall over an ancient volcanic cliff in the west of Scotland. I don't like using it during farming season because I worry about what the farmers might be pumping into it. However, the volume of water than falls from the cliff in dryer weather forms a small burn about one foot wide and two to three inches deep and it is right on the edge of the shore. The amazing thing about this water source is it is not tidal and it passes over the cliff through masses of weedy moss. I don't know which type of moss it is. Then it runs through more smooth volcanic rock which is covered in moss, then over pebbles and trickles over into miniature waterfalls like a slow running kitchen tap where it is crystal clear. The area surrounding the larger falls is shade and there is a feast of salad ingredients all around it - wild garlic, and goose grass etc. It is an oasis, really beautiful. Well I collected some samples of clean water, the clearest from where the water had already passed through the moss and where it trickled over some rocks, and some from the mossy area and some from a tiny more stagnant pool. I took some moss samples with two of the water samples and none with the clear water. I placed the moss sample with some water from the stagnant water onto a slide with a coverslip and I have had an amazing result. I have found an unknown creature, unknown to me, which if I were not looking under a microscope at 40x mag, I would swear it was a caterpillar. At that mag, 4x obj, 10x eyepiece, its length is almost exactly half the diameter of the eyepiece lens as I look through it. It is much larger than the diatoms I saw, and it appeared to be feeding two babies. It moved like a caterpillar among the moss but had no legs. it has two tentacles from its tail end with five filaments from each tentacle an then a flute like tail with much smaller tentacles on it. The body was segmented like a caterpillar with 11 segments, I counted, but could be mistaken. The head had two very small antennae and where the head joins the body, on its underside it had a protrusion of some kind, like a reproductive organ. Light from above showed it to be green in colour and the head dark brown to black. Light from below showed it to be transparent. One dim light from the side showed it to be transparent with a dark coloured head. The green appearance may have been coming off of the reflection from the green, weedy moss. Do you have any clues to its identity? I did a bit of searching and found something that very closely resembles it: 'Diamesa Bohemani larva.' It is not identical to what I see, but it is damn close. Actually it kind of is identical. The direction of angle and lighting can throw us off a bit. It looks amazing with just a very dim, soft light from underneath and some stronger light from the top to reflect the green of the moss. I should have said, I have measured the little creature at about 2 mm long.

[@Dayv7]

Looks like a Tardigrade at 5.45 just left of centre.

[@peterashcroftq1494]

Loving it, I’ve just begun practicing microscopy and finally received my first slides so off to the pond this weekend with my son to find some of these amazing creatures. Thanks sir

[@wetdroidedition2549]

3:57 Is there a tardigrade at 8 in the fork of algae?

[@corkycorner3232]

This channel it great I'll also visited your home country this year, beautiful and keep these entertaining videos coming. Thanks

[@adventureofknowledge663]

Hello intelligent KZbinr thanks for great video and great topics 👍👍👍👍👍

[@atulanand1595]

I love your videos. I would like to request you to mention the magnifications for each video, it will help budding microbiologists and compare results.

[@Microbehunter]

The magnification issue is a problem. I can tell you which objective I am using, for sure, but the camera optics also crops something away and sometimes I will zoom in digitally to fill up the screen better.

[@Alexandre13026]

More vídeos, tks for every video. Wait next time.

[@juniperjames718]

I think the organism at 7:55 is a vorticella that's oriented directly towards the camera, so its tail isn't visible. The "mouthparts" inside the ring of cilia look very similar to other vorticella I've seen

[@sebaaullador1292]

Can you see these type of things with a digital microscope? Like an Andonstar Adsm302 or a Tomlov TM-DM11

[@Tony12097]

Do you think those unknown egg-shaped dots are pollen?

[@austinred458]

Wow.... Which microscope do you use? Great video 😍😍😍

[@plaaxys]

Im new to this stuff and im only seeing about 3-5 of those swimming thingies.

[@dougb1542]

Hi! Love your channel. Very informative and helpful. You had mentioned a "identification guide" in the video. I would like to know which guide book you use if you would be so kind.

[@Microbehunter]

This one here: www.amazon.de/Das-Leben-Wassertropfen-Heinz-Streble/dp/344015694X It does not matter that it is in German, the identification is over drawings, and when you have the name, then you can google for more information.

[@chaudry123]

Hi microbhunter, Thanks for micro national geographic channel 😂

[@snowshoe740]

Its interesting.... make more videos

[@doctard2990]

why do some of the organisms keep going in circles?

[@christophermclaughlin8899]

Doctard responding to some chemical gradient indicating potential food sources maybe?

[@adrenochrome_slurper]

Maybe their sensory organs can't function properly being pinned down under glass.

[@arvetis]

The way some microorganisms move is to keep turning randomly at various intervals depending on how much they like their current surroundings (oxygen levels, light, chemical stimulus, etc.). The more they like their current surroundings, the faster they turn, so as to stay in the same general area. When they don't like their surroundings, they turn less, so they'll get out of the area faster.

[@wahabvlogger1651]

hallo sir i like your videos i requst you to prepare slide of onine root tip

[@rooneyrythm]

Cool. Can we do some more bacteria next?

[@newpersia88]

are these colors the original colors of those microorganisms?

[@paulfarrell5935]

Oliver, you mentioned your “identification book”. What book is that and where can it be purchased?

[@Microbehunter]

www.amazon.com/dp/344015694X/?cv_ct_id=amzn1.idea.3DKTNDAL7KB1C&cv_ct_pg=storefront&cv_ct_wn=aip-storefront&ref=exp_cov_microbehunter_dp_vv_d It is in German, though.

[@paulfarrell5935]

Microbehunter, Thank you sir!

[@andreyzomin9418]

Can I see this with cheap microscope you reviewed earlier?? What are the requirements

[@pauldiaz4273]

Awesome, i can’t seem to get my darkfield setup right! Any help?

[@What-the-meow-meow]

He has another channel for microscope techniques. This channel is more observations.

[@LeKikoojap]

I've tried to collect microplankton on the beach (sea water) but there was not much, are there special techniques for plankton?

[@Microbehunter]

Plankton net!

[@glasslinger]

I didn't have to go to the lake! I went to my bath tub! :)

[@What-the-meow-meow]

Clean your bath tub! :3

[@mrwhats-his-name8956]

Eeuuuurgh!

[@oscarmauriciofaguada7929]

Hello! What is the identification book that you use?

[@Microbehunter]

www.amazon.com/Das-Leben-im-Wassertropfen/dp/344015694X/ref=sr_1_3?dchild=1&keywords=das+leben+im+wassertropfen&qid=1612372673&sr=8-3

[@alberto148]

@microbehunter I noticed you have a bit of chromatic aberration on the image, it's most noticeable in areas of high contrast, is there a way to improve that? I've seen other microscopy channels with higher magnifications that don't seem to have as high levels of aberration.

[@Macieks300]

how do you correct that?

[@saumilsunilshah5663]

Where do you see chromatic abberation ?

[@israelramos7441]

Whenever you can please fix some tissue samples in paraffin

[@fukkdasystemxd3759]

You should to the red tide algae

[@komalsingh9248]

Who is in love with seeing mircobes😍😍

[@vesbadri]

How much magnification did you use pls reply

[@Microbehunter]

I have been using 10x to 40x objectives.

[@ferdinandfressflash6320]

Die Glockentierchen kontrahieren wenn man ganz ganz sachte ans Mikroskop tippt

[@runito75]

What was the name of the animal 5 minutes in?

[@Microbehunter]

Vorticella

[@damontsekos365]

One thing puzzles me!..You've stated that you have a university degree, in research microbiology, and molecular biology and that you teach biology in secondary school!..How is it possible then that in many occasions you are looking at living organisms under the microscope and don't know what they are?

[@Microbehunter]

Because bacteria and some other microorganisms simply can not be identified down to the species level by microscopy alone. Much also depends to what degree of detai you want to identify it. Sometimes you need to do DNA tests etc. to go down to the species level. Of course it is often easy to look at a microbe and then identify it as a ciliate, diatom, etc. but these are broad categories. Sometimes it is possible to identify protozoa down to the genus level by looking at it, but individual species often becomes difficult. You can not identify the brand of a car by looking at the color alone, no matter how much you know about cars. Eg. if you see a eukaryotic single celled microorganism with one flagellum, then it is a flagellate. There are thousands of them and many of them have not even been characterized or described.without further studies (staining etc) it is not possible to identify it to the species level. Bacteria are specially problematic here. Microscopy has a quite limited use in bacterial identification. Protozoa are much better here.

[@lotharmayring6063]

The resolution in darkfield is higher than in brightfield. You should know this

[@Microbehunter]

the resolution is determined by the numerical aperture and the wavelength and not by the presence of a darkfield patch stop. The fact that you are able to see structures smaller than the resolution limit in darkfield has to do something with the contrast. But the structure itself will only be visible as a diffraction pattern and itself will not be resolved. In other words, the small structure will be seen as a larger blurry spot.

[@lotharmayring6063]

@@Microbehunter this is only a theory, in practice the resolution is better in darkfield than in brightfield, that is fact

[@lotharmayring6063]

@@Microbehunter num ap is n sin alpha. Both n an alpha are affected by the condensor and lieght source

[@lotharmayring6063]

@@Microbehunter interference microscopy like darkfield have better resolution

[@AbyssalManta]

I always hear him say "Micro-Punter" for some reason.

[@redf7209]

Just tried the same thing yesterday, Virtually no sign of life .

[@autazm4782]

The music was unnecessary in my opinion, please do not insert it if possible. Not sure what others think about this though.

[@Macieks300]

Yeah, it's ok for intro and outro but it didn't fit the other parts of the video.

[@christophermclaughlin8899]

Some Bach would have been my choice, or maybe Chopin or Grieg but to each their own.

[@Roger__Wilco]

i would have chosen AC/DC

[@What-the-meow-meow]

Who cares. I enjoyed it.