3 prime polyadenylation of eukaryotic mRNA | eukaryotic RNA processing | RNA poly adenylation

[@simonsteen-andersen3181]

Thank you, for providing this overview! It would be interesting to study the mRNA 3-prime polyadenylation on the IL12RB2 gene, which,- in addition to its well studied role in the IL-12/IL12RB1+IL12RB2/STAT4/IFN-gamma pathway and, thus, in anti-mycobacterial immunity,- could play an essential role in IL-2 signaling and the epigenetic modifications taking place as a result of DC-T-cell crosstalk and TCR activation before the clonal expansion of Ts, since IL12RB2 mRNA and protein expression is upregulated by IL-2-induced STAT5 activation and has been shown to mediate the demethylase of H3K27me3/2 via JMJD3-binding on three locations of the intronic regions of IL12RB2. JMJD3, also, mediates Brg1 and, thus, plays a non-reduntant role in the SWI/SNF complex in cooperation with the T-bet mediated H3K4me3 marker essential for chromatin elongation and chromatin accessability for important transcription factors which are necessary for adapting both a TH1 and Treg specific gene signature and might even play an essential role in the cross-priming of cytotoxic CD8+ Ts necessary for tumor control. The role of IL12RB2 as an interface between molecular and cellular mechanisms during T-cell differentiation; between innate and adaptive immunity; between T-cell-mediated maturation of DCs via CD40L signaling and allowing for a correct negative regulation of the type 1 IFN feedback-loop while balancing the transition from a stem-like to a fully differentiated gene signature via mediation of chromatin accessability together with T-bet/SWI-SNF complex, might be intersting to further study regarding the mRNA 3-prime polyadenylation, since a dysregulation of this step-wise mechanism via mutations on the first exons and/or introns of the IL12RB2 gene holds the potential to be of great importance for understanding immunodefeciency, autoimmunity and cancers,- exemplified by the fact that the IL12RB2 knock-out mice model develops a complex pathophysiological presentation with immunodefeciency, spontanious autoimmunity and cancers....

[@snakepliskin6391]

Absolutely love these videos! They are advanced for me but very clear! I did have a couple questions though. Does splicing happen before, during, or after polyadenylation? Also where exactly do the endonucleases cleave? You mentioned that after the cleavage the mRNA was freed from the DNA for the most part so is it almost like a helicase going down the middle to separate them? Or is it cleaving a small part after the AAUAAA sequence that allows polyadenylation to occur? Thanks so much!

[@manishahazra544]

hey ! you didnt talk about CPSF ( cleavage and polyadenylation specificity factor ) and CSTF ( cleavage stimulation factor ) .

[@shilpasatapathy6665]

He said that in previous videos

[@lakhanprajapati6933]

First view 🎉 first comment 😊

[@shomusbiologyofficial]

Thank you

[@simadassaikia8588]

Always Hi not Bye Sir ❤

[@Chinar93]

hi sir plz let me know which topics are important from biochemistry and enzyme kinetics

[@shomusbiologyofficial]

Enzyme kinetics and enzyme inhibition

[@rabisankar787]

😊

[@Shinchan2700-i9i]

Why only adenine residue is added in poly A tail, not guanine cytosine or urecil

[@yoongayyy124]

because it is Poly A polymerase enzyme, simple polymerase can add all the nucleotides but poly A will only add Adenine

[@sahilhoque1645]

Good explanation

[@shomusbiologyofficial]

Thank you

[@ranjitotari5988]

🥰

[@shomusbiologyofficial]

Thank you