3D Protein Modeling: X-Ray Diffraction and PyMOL Tutorial

[@robertplautz9722]

thank you for this very insightful video. a few questions: 1. crystallization or proteins process: a. how do you know that it is proteins and not something else that is crystallizing? b. how many proteins are in a crystal undergoing xray diffraction? if more than one, how can the computer analyze multiple proteins simultaneously (probably it isnt, but how do you know)? 2. xray diffraction: the crystal is being rotated. so the dots appear on the screen. how do you know which dots were produced at what degree of rotation? (it probably has something to do with Bragg's law?). is it important to know what degree of rotation each dot "represents"? other question: i saw another vid (British lab, which used Diamond complex for analysis) where they were preparing proteins for crystallization. there was a process called "dilution" of the proteins, to make sure there was a high concentration of protein. what is that all about? isnt a single crystallized protein necessary for the xray diffraction? (this of course is linked with another question stated above.) it is all so mysterious, that all of this is known, and that the technology of xray diffraction and computerized analysis has been developed with such a surety of the results! is it a kind of magic; that is, what are the larger implications about matter and energy here, and their relationship to mathematics? of course this is outside the range of your video, and perhaps your own interests. i wish you were my teacher!