Protein Crystals - Backstage Science

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Пікірлер: 41

@boyinapeatbog 12 жыл бұрын

I see science, I dream science, I breath science. Science runs through my veins and through my soul. Science OWNS my soul. NEED MORE SCIENCE.

@jeebersjumpincryst 12 жыл бұрын

Bio has never held the interest that phys and chem has for me, but that blew me away. Amazing amazing amazing

@MrComaToes 12 жыл бұрын

I feel like the fellows at Lawrence Livermore, Cal-Tech, MIT and countless others are scrambling to find their own "Brady", and if not, they are missing out. As a 55 year old fan of the Sciences I have witnessed some incredible breakthroughs during my lifetime. To see the inner workings as shown so brilliantly here on BackstageScience, is to have the chance to see the proverbial tip of the scientific sword. Bravo Brady

@RMoribayashi 12 жыл бұрын

One of the reasons the crystals are so small is that as they grow they remove material from the solution near the crystals, changing the density of the solution near the crystal. Gravity causes convection currents to form in the solution as the less dense parts rise, deforming larger crystals. That's why they do a lot of protein crystal growth experiments in space. The lower convection in a microgravity environment allows larger crystal growth and also larger proteins to be crystallized.

@DracoMhuuh 12 жыл бұрын

I just learned about Fourier Analysis and convolution, diffraction at our university and it's fascinating to see how it is used across all scientific fields, truly powerfull concepts

@OmegaPaladin144 11 жыл бұрын

That's a good question. Typically, you add in an antibiotic resistance gene along with your protein, then use select for only those that have the vector or "vehicle". That might eliminate some of the mutants. From my experience, the process of inserting a gene vector and turning into protein has a notable failure rate, and the natural mutation of the bacteria may be part of that.

@13someguy13 12 жыл бұрын

The cool thing about this video is that I've already had the opportunity to do protein generation and purification with HYRS when I was 16.

@SuperibyP 12 жыл бұрын

PCR was written on the block of test tubes at about 00:44, does that stand for Polymerase Chain Reaction, the process by which DNA can be replicated outside of living tissue?

@madsciclare 10 жыл бұрын

I really love this video! I'm interested in protein crystals but there's so little information that I can find

@petros_adamopoulos 12 жыл бұрын

Do crystallized proteins retain their shape and how well? I'm thinking in particular of active sites that are dependent on the ternary structure.

@boldger13 12 жыл бұрын

One of the best videos yet!

@BackstageScience 12 жыл бұрын

cool... cheers for watching

@ma356289 12 жыл бұрын

the first video in youtube without dislikes

@piranha031091 12 жыл бұрын

Wow, I wasn't aware that complex macromolecules like proteins could crystallize so nicely!

@OmegaPaladin144 11 жыл бұрын

Yeah - it's the first step in producing enough of your gene so that you can add it to the vector or "vehicle" he was talking about. I made (or attempted to make - this is not anywhere near easy) proteins in grad school like that

@DaffyDaffyDaffy33322 12 жыл бұрын

Subed as soon as I realized Brady was making these videos :)

@MrTinnPeach 12 жыл бұрын

Really interesting video. Rather than putting these sort of videos here you should make a new channel about biology. There are some fascinating processes that happen at the cellular level.

@MsTrance1 12 жыл бұрын

Does anyone Know that if you use gene insertion in bacteria to produce proteins, does the nature mutation accumulation have any effect on the results?

@poelfkat 12 жыл бұрын

great couldn't you have released this vid 2 weeks earlier then I could have used it as source in my report about DNA and Proteins Anyway thanks for uploading