4.4. Next Generation Sequencing - Practice Session : Variant Calling

[@JenniFadoni]

Very clear explanation. Thank you very much

[@camilocarias5501]

Hello, how do I determine the correct filtering threshold in VCFfilter? There is a minimum threshold value? Should I use 200 as a correct filtering threshold or can I use a 220 based on my first result?

[@dylandog2841]

From a multisample vcf file how can get only a subset heterozygous (1/2) using VCFfilter?

[@elenips7231]

Very helpful video , thank you!! I am not really familiar with bioinformatics and in this part of my project, I am trying two compare two VCF files corresponding to the results of healthy tissue and tumor tissue. I want to compare these VCF files and remove their similarities. More specific I want to remove the information of the healthy tissue from the tumor one. Have you any suggestions on which tool I should use or any way that I can do my analysis? thank you in advance!

[@niusha3323]

Anyone else here hopeless after the coursera project? Lol

[@yasmeenomar3639]

totally lol

[@nkosinathinxumalo7643]

i need assistance on that please help

[@AyrodsGamgam]

bioinfo is overrated, lots of tools become obsolete,.