A quick and simple self-removing affinity tag with Prof. David Wood

David Wood is a professor of Chemical Engineering at Ohio State University and is a scientific co-founder at Protein Capture Science. David completed a double major in Biology and Chemical Engineering at Caltech, and then worked at Amgen on the Neupogen process in the early 1990’s. He completed a Ph.D. in Chemical Engineering at Rensselaer Polytechnic Institute, and worked briefly at Bristol Meyers Squibb before starting his career in academia at Princeton University. Over the past 25 years he has developed a self-removing tag technology with the goal of creating a disruptive downstream processing platform for the biopharma industry.
Key Takeaways of the Masterclass
• A self-removing affinity tag can provide highly purified proteins in a single step based on a universal platform.
• Tag removal required only a pH change, where the elution buffer can contain a wide range of buffers, salts, polymers and detergents.
• The technology can be used at any scale, from basic research through full-scale manufacturing, and a magnetic bead should be available this summer.