Thank you very much :)

You are welcome :)

Our standardbeads are all resuseable. He have regeneration protocols for all of them :)

@@CubeBiotech thanks :)

It is a trade-off. Higher affinity means higher vield, but that usually comes with less purity/specificity. Because if you make it easier to bind, false targets also get a chance to bind. And in the other direction this applies as well. If you make it harder to bind - higher specificity, you get purer results but also less yield.

Thank you :)

Thank you :)

Thank you :)

Thank you for providing these additional information :)

Thank you :)

Glad that we could help you there. Did you mean something specifically?

@@CubeBiotech just the whole process in general! I had to do an oral presentation and this was one of the main topics!

Thank you :)

Hi Patrick, thank you for the positive feedback! That is always appreciated :)

We are sorry to hear that. All videos after this one are narrated. We hope you still found the information provided here useful.

Thank you very much. We are really glad to hear that!

No we are a biotech company :)

You are welcome :)

We are glad that we could help you. These videos take time to make, and we currently have a longer list we like to work on first. But you recommendation is considered :)

Time stamps are set :)

Noted and thank you :)

Good Luck !

A very annoying sticky ball.

There are no slides, sadly. These are, for the most part, Adobe After Effects files. :(

We are happy it helped you. What part of the video did you find the most helpful?

Thank you :)

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Be assured. This is the correct protocol for handling our SMALP products. :)

Thank you very much for these kind words! That definetly made the day of the lead creator of this video! Thank you for further recommending us!

You are welcome :)

Thank you :) We hope it well help you in the future.

Ask the guy who made that video :P

*Says the chemical combination of titin*

Glad that we could help :)

Thank you very much as well :) Glad to see that the effort pays off.😊

Hello :) You mean if just a single purification method is enough? His-tag purifications always leave some impurities behind. That is the trade-off for its incredible protein yield. In-house we usually follow-up with a Size-Exclusion chromatography if the His-tag purification was not enough.

I bet u are from fbi

Nope. Germany :)

Glad that we could help. And yes this sounds reasonable. Normal Imidazole concentrations in His-tag elution buffers is 500µM. This almost certainly must be washed away first through dialysis for any downstream animal tests.

Really Glad to hear that. It makes us happy that the effort was worth it!

@@CubeBiotech 💜

Good Day :) We are experts on protein purification and not the Golgi-Apperatus. We do not feel comfortable to answer this for you. What we do recommend to ask this question on platforms like Researchgate. You should find your answer there.

Thank you so much for your guidance!!!

We are glad that you like it :)

Thank you! Glad that you liked it.

Yes that is what was said. "The protein is just soluble."

@@CubeBiotech thanks a bunch

@@elizabethmonday3751 You are welcome :)

It is not really shown but that is the buffer flowing through at around time 1:22, so just catch that.

Glad that you like it!