Agar update!

  Рет қаралды 2,645

Psych-E

Psych-E

Күн бұрын

Пікірлер: 15
@surfj9009
@surfj9009 Жыл бұрын
If you’re getting contams in your cups, try doing less cups at once. Do 5, put lids on, do another 5, put lids on, etc. And don’t lean over them. Bacteria can fall off your arms and sleeves into cups below. Minimize the time the cups are open and exposed and your success rate will go up
@Psych-Efunguy
@Psych-Efunguy Жыл бұрын
Thank you for the feedback and comment, very appreciated. Ideally, I'd like to get a temperature device to see exactly what the agar temp is. I've ran into the issue of having condensation on lids with that method (also tried using hot towel/plate on top to avoid condensation). The method I used in this video is what I found to work best for quantity, but i agree doing less at a time would have more precautions and most likely increase success rate.
@marlonglodo1930
@marlonglodo1930 Жыл бұрын
Hello sir. Why not use magnetic stirrer. Instead of swirling the botlle..
@Psych-Efunguy
@Psych-Efunguy Жыл бұрын
I have not invested in them in the past. I appreciate the comment, time to invest in a better hotplate and magnetic stirrers!
@marlonglodo1930
@marlonglodo1930 Жыл бұрын
One more thing. Is it posible to inject liquid culture direct to fruiting bag? Thank you
@Psych-Efunguy
@Psych-Efunguy Жыл бұрын
@@marlonglodo1930 I would say it depends on the culture and what your inoculating. I would not recommend it, I prefer going to grains, then to fruiting substrate. If you are doing BRF cakes it would be fine and probably better than going from spore syringe. Oysters might also get away with it as they are pretty aggressive. Hope this helps!
@marlonglodo1930
@marlonglodo1930 Жыл бұрын
@@Psych-Efunguy ohh thank you. iam planning to grow volva mushroom in my understanding it is not so sensitive like oyster. And it can gain more profit rather than oyster here in my country..
@Psych-Efunguy
@Psych-Efunguy Жыл бұрын
@@marlonglodo1930 that's great! If you try it let me know how it goes.
@AnxiousStacker
@AnxiousStacker 2 жыл бұрын
Hey man I'm a new sub. Like your set up a lot. Hey did you build your flow hood? How good is it, what is the exact filter and blower, and specs of the box? I definitely want to get a flow hood but I'm living on a VERY limited income. I want to start a channel one day showing my journey and I've had the plan of buying one of those grow rooms for all my lab work
@Psych-Efunguy
@Psych-Efunguy 2 жыл бұрын
Thank you for the support! I did build my flow hood, I built a few actually. I'll do a video to help out, thank you for the suggestion. As for cost, the box is mostly inexpensive, your filter will be your biggest spending, and the blower, although not cheap shouldn't be too bad. Check out @Willy's World on KZbin, I believe he has a video for a very inexpensive build.
@AnxiousStacker
@AnxiousStacker 2 жыл бұрын
@@Psych-Efunguy Yeah I like that one he built but that filter is unavailable for some reason. Definitely in aww of you guys working with freedom of movement not having to use a SAB. Good stuff man
@Gulashinicove
@Gulashinicove 2 жыл бұрын
HEY MAN.BOTTLE SIZE?
@Psych-Efunguy
@Psych-Efunguy 2 жыл бұрын
Quart mason jar, wide mouth.
@jaysizzle3023
@jaysizzle3023 Жыл бұрын
Your jars are aren’t suppose be in the water. Suppose rise above
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